Journal: Journal of Oncology

Article Title: miR-455 Inhibits the Viability and Invasion by Targeting RAB18 in Hepatocellular Carcinoma

doi: 10.1155/2021/9923454

Figure Lengend Snippet: Primer sequences for RT-qPCR.

Article Snippet: The primary antibodies were RAB18 (1 : 1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), E-cadherin, N-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Techniques:

Journal: Journal of Oncology

Article Title: miR-455 Inhibits the Viability and Invasion by Targeting RAB18 in Hepatocellular Carcinoma

doi: 10.1155/2021/9923454

Figure Lengend Snippet: miR-455 targets RAB18 and regulates its expression. (a) TargetScan predicted that RAB18 was a potential target of miR-455. (b) miR-455 mimic reduced the luciferase ability of wild type mRNA 3′-UTR, whereas it did not alter the mutant 3′-UTR vs WT-NC, ∗ P < 0.05 and vs MUT-NC, # P < 0.05. (c) The expression of RAB18 was decreased by miR-455 mimic, while it was promoted by miR-455 inhibitor in Huh7 cells vs mimic NC, ∗ P < 0.05 and vs inhibitor NC, ∗ P < 0.05.

Article Snippet: The primary antibodies were RAB18 (1 : 1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), E-cadherin, N-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Techniques: Expressing, Luciferase, Mutagenesis

Journal: Journal of Oncology

Article Title: miR-455 Inhibits the Viability and Invasion by Targeting RAB18 in Hepatocellular Carcinoma

doi: 10.1155/2021/9923454

Figure Lengend Snippet: RAB18 restores partial functions of miR-455 on cell viability and invasion. (a) RAB18 was overexpressed in HCC tissues versus nontumor tissues vs NT, ∗ P < 0.05. (b) The expression of RAB18 was higher in HCC-LM3, Huh7, and Bel-7402 than in L-O2 cells vs L-O2, ∗ P < 0.05; ∗∗ P < 0.01. (c) RT-qPCR revealed the transfection efficiency of overexpressing RAB18 in miR-455 mimic-tranfected cells vs miR-455 mimic, ∗ P < 0.05. (d) RAB18 reversed partial functions of miR-455 on cell viability vs miR-455 mimic, ∗ P < 0.05. (e) Overexpressing RAB18 improved cell invasive ability in miR-455 mimic-transfected cells vs miR-455 mimic, ∗ P < 0.05.

Article Snippet: The primary antibodies were RAB18 (1 : 1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), E-cadherin, N-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Techniques: Expressing, Quantitative RT-PCR, Transfection

Journal: Molecular Endocrinology

Article Title: Nr4a1 siRNA Expression Attenuates α-MSH Regulated Gene Expression in 3T3-L1 Adipocytes

doi: 10.1210/me.2010-0231

Figure Lengend Snippet: Analysis of mouse metabolic gene expression in 3T3-L1 adipocytes treated with NDP-MSH

Article Snippet: Rab18-Mm00441057_m1 , −6.68724133206513 , 1.56004 , 0.17812 , 0.26313 , 0.80857 , −0.0922824175596581 , Valid.

Techniques: Gene Expression

Journal: Molecular Endocrinology

Article Title: Nr4a1 siRNA Expression Attenuates α-MSH Regulated Gene Expression in 3T3-L1 Adipocytes

doi: 10.1210/me.2010-0231

Figure Lengend Snippet: Analysis of mouse metabolic gene expression in 3T3-L1 adipocytes treated with 8-Br cAMP

Article Snippet: Rab18-Mm00441057_m1 , −6.68724133206513 , 1.56004 , 0.17812 , 0.26313 , 0.80857 , −0.0922824175596581 , Valid.

Techniques: Gene Expression