r5p Search Results


r5p fluka  (Fluka Chemical)
90
Fluka Chemical r5p fluka
R5p Fluka, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r5p fluka/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
r5p fluka - by Bioz Stars, 2026-03
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r5p  (Sangon Biotech)
90
Sangon Biotech r5p
Plk1 enhances PPP and biosynthesis in cancer cells. a Using [U- 13 C]-labeled glucose, M + 5-labeled <t>R5P</t> produced from PPP was detected by LC-MS. HeLa cells were synchronized by double HU block. When the cells were treated for the second round of HU block for 11 h, i.e., 1 h before releasing, U- 13 C glucose was added into the medium and further culture for 1 h and then harvested for G1 phase cell analysis. For cell samples of S and G2/M phases, after the regular second round of HU block for 12 h, cells were released into fresh HU-free medium and further cultured for 4 and 9 h, respectively, followed by supplementation of U- 13 C glucose and 1 h culture before harvesting cells for analysis. Cells were harvested and 13 C-incorporated R5P was detected by LC-MS. The calculation of % of the m + 5 isotopologue of ribose is % of the sum of isotopologues. b – f The PPP metabolites were detected by NMR. Cells were cultured in medium containing [2- 13 C]- or [U- 13 C]-labeled glucose. The graphic description shows that by using the [2- 13 C]-labeled glucose, NMR measurement could distinguish the lactate produced from PPP and that derived from the general glycolysis ( b ). Lactate derived from the oxidative PPP was calculated by the total lactate multiplied with the ratio of 3- 13 C lactate detected by NMR in Plk1 overexpression ( c ) or knockdown cells ( d ). Using the [U- 13 C]-labeled glucose, R5P and some downstream nucleotides generated from the PPP flux were detected by NMR in Plk1 overexpression ( e ) or knockdown cell lines ( f ). To induce Plk1 shRNA expression, cells were grown in the presence of 0.1 μg/ml doxycycline for 72 h. The calculation of isotopologue of ribose is total intensity of 13 C-labeled ribose moiety. g U- 13 C glucose metabolic flux assays were performed in Plk1-overexpressing HeLa cells with G6PD knockdown by NMR. The results were normalized to cell numbers or cell wet weight. Data were represented as the mean ± s.d. or s.e.m. * P < 0.05 as compared to indicated group by two-sided Student’s t -test
R5p, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r5p/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
r5p - by Bioz Stars, 2026-03
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barium salt r5p  (Tokyo Chemical Industry)
90
Tokyo Chemical Industry barium salt r5p
Plk1 enhances PPP and biosynthesis in cancer cells. a Using [U- 13 C]-labeled glucose, M + 5-labeled <t>R5P</t> produced from PPP was detected by LC-MS. HeLa cells were synchronized by double HU block. When the cells were treated for the second round of HU block for 11 h, i.e., 1 h before releasing, U- 13 C glucose was added into the medium and further culture for 1 h and then harvested for G1 phase cell analysis. For cell samples of S and G2/M phases, after the regular second round of HU block for 12 h, cells were released into fresh HU-free medium and further cultured for 4 and 9 h, respectively, followed by supplementation of U- 13 C glucose and 1 h culture before harvesting cells for analysis. Cells were harvested and 13 C-incorporated R5P was detected by LC-MS. The calculation of % of the m + 5 isotopologue of ribose is % of the sum of isotopologues. b – f The PPP metabolites were detected by NMR. Cells were cultured in medium containing [2- 13 C]- or [U- 13 C]-labeled glucose. The graphic description shows that by using the [2- 13 C]-labeled glucose, NMR measurement could distinguish the lactate produced from PPP and that derived from the general glycolysis ( b ). Lactate derived from the oxidative PPP was calculated by the total lactate multiplied with the ratio of 3- 13 C lactate detected by NMR in Plk1 overexpression ( c ) or knockdown cells ( d ). Using the [U- 13 C]-labeled glucose, R5P and some downstream nucleotides generated from the PPP flux were detected by NMR in Plk1 overexpression ( e ) or knockdown cell lines ( f ). To induce Plk1 shRNA expression, cells were grown in the presence of 0.1 μg/ml doxycycline for 72 h. The calculation of isotopologue of ribose is total intensity of 13 C-labeled ribose moiety. g U- 13 C glucose metabolic flux assays were performed in Plk1-overexpressing HeLa cells with G6PD knockdown by NMR. The results were normalized to cell numbers or cell wet weight. Data were represented as the mean ± s.d. or s.e.m. * P < 0.05 as compared to indicated group by two-sided Student’s t -test
Barium Salt R5p, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/barium salt r5p/product/Tokyo Chemical Industry
Average 90 stars, based on 1 article reviews
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ribose-5-phosphate (r5p)  (Nacalai)
90
Nacalai ribose-5-phosphate (r5p)
Plk1 enhances PPP and biosynthesis in cancer cells. a Using [U- 13 C]-labeled glucose, M + 5-labeled <t>R5P</t> produced from PPP was detected by LC-MS. HeLa cells were synchronized by double HU block. When the cells were treated for the second round of HU block for 11 h, i.e., 1 h before releasing, U- 13 C glucose was added into the medium and further culture for 1 h and then harvested for G1 phase cell analysis. For cell samples of S and G2/M phases, after the regular second round of HU block for 12 h, cells were released into fresh HU-free medium and further cultured for 4 and 9 h, respectively, followed by supplementation of U- 13 C glucose and 1 h culture before harvesting cells for analysis. Cells were harvested and 13 C-incorporated R5P was detected by LC-MS. The calculation of % of the m + 5 isotopologue of ribose is % of the sum of isotopologues. b – f The PPP metabolites were detected by NMR. Cells were cultured in medium containing [2- 13 C]- or [U- 13 C]-labeled glucose. The graphic description shows that by using the [2- 13 C]-labeled glucose, NMR measurement could distinguish the lactate produced from PPP and that derived from the general glycolysis ( b ). Lactate derived from the oxidative PPP was calculated by the total lactate multiplied with the ratio of 3- 13 C lactate detected by NMR in Plk1 overexpression ( c ) or knockdown cells ( d ). Using the [U- 13 C]-labeled glucose, R5P and some downstream nucleotides generated from the PPP flux were detected by NMR in Plk1 overexpression ( e ) or knockdown cell lines ( f ). To induce Plk1 shRNA expression, cells were grown in the presence of 0.1 μg/ml doxycycline for 72 h. The calculation of isotopologue of ribose is total intensity of 13 C-labeled ribose moiety. g U- 13 C glucose metabolic flux assays were performed in Plk1-overexpressing HeLa cells with G6PD knockdown by NMR. The results were normalized to cell numbers or cell wet weight. Data were represented as the mean ± s.d. or s.e.m. * P < 0.05 as compared to indicated group by two-sided Student’s t -test
Ribose 5 Phosphate (R5p), supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r5p  (Fluka Chemical)
90
Fluka Chemical r5p
Plk1 enhances PPP and biosynthesis in cancer cells. a Using [U- 13 C]-labeled glucose, M + 5-labeled <t>R5P</t> produced from PPP was detected by LC-MS. HeLa cells were synchronized by double HU block. When the cells were treated for the second round of HU block for 11 h, i.e., 1 h before releasing, U- 13 C glucose was added into the medium and further culture for 1 h and then harvested for G1 phase cell analysis. For cell samples of S and G2/M phases, after the regular second round of HU block for 12 h, cells were released into fresh HU-free medium and further cultured for 4 and 9 h, respectively, followed by supplementation of U- 13 C glucose and 1 h culture before harvesting cells for analysis. Cells were harvested and 13 C-incorporated R5P was detected by LC-MS. The calculation of % of the m + 5 isotopologue of ribose is % of the sum of isotopologues. b – f The PPP metabolites were detected by NMR. Cells were cultured in medium containing [2- 13 C]- or [U- 13 C]-labeled glucose. The graphic description shows that by using the [2- 13 C]-labeled glucose, NMR measurement could distinguish the lactate produced from PPP and that derived from the general glycolysis ( b ). Lactate derived from the oxidative PPP was calculated by the total lactate multiplied with the ratio of 3- 13 C lactate detected by NMR in Plk1 overexpression ( c ) or knockdown cells ( d ). Using the [U- 13 C]-labeled glucose, R5P and some downstream nucleotides generated from the PPP flux were detected by NMR in Plk1 overexpression ( e ) or knockdown cell lines ( f ). To induce Plk1 shRNA expression, cells were grown in the presence of 0.1 μg/ml doxycycline for 72 h. The calculation of isotopologue of ribose is total intensity of 13 C-labeled ribose moiety. g U- 13 C glucose metabolic flux assays were performed in Plk1-overexpressing HeLa cells with G6PD knockdown by NMR. The results were normalized to cell numbers or cell wet weight. Data were represented as the mean ± s.d. or s.e.m. * P < 0.05 as compared to indicated group by two-sided Student’s t -test
R5p, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r5p/product/Fluka Chemical
Average 90 stars, based on 1 article reviews
r5p - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Plk1 enhances PPP and biosynthesis in cancer cells. a Using [U- 13 C]-labeled glucose, M + 5-labeled R5P produced from PPP was detected by LC-MS. HeLa cells were synchronized by double HU block. When the cells were treated for the second round of HU block for 11 h, i.e., 1 h before releasing, U- 13 C glucose was added into the medium and further culture for 1 h and then harvested for G1 phase cell analysis. For cell samples of S and G2/M phases, after the regular second round of HU block for 12 h, cells were released into fresh HU-free medium and further cultured for 4 and 9 h, respectively, followed by supplementation of U- 13 C glucose and 1 h culture before harvesting cells for analysis. Cells were harvested and 13 C-incorporated R5P was detected by LC-MS. The calculation of % of the m + 5 isotopologue of ribose is % of the sum of isotopologues. b – f The PPP metabolites were detected by NMR. Cells were cultured in medium containing [2- 13 C]- or [U- 13 C]-labeled glucose. The graphic description shows that by using the [2- 13 C]-labeled glucose, NMR measurement could distinguish the lactate produced from PPP and that derived from the general glycolysis ( b ). Lactate derived from the oxidative PPP was calculated by the total lactate multiplied with the ratio of 3- 13 C lactate detected by NMR in Plk1 overexpression ( c ) or knockdown cells ( d ). Using the [U- 13 C]-labeled glucose, R5P and some downstream nucleotides generated from the PPP flux were detected by NMR in Plk1 overexpression ( e ) or knockdown cell lines ( f ). To induce Plk1 shRNA expression, cells were grown in the presence of 0.1 μg/ml doxycycline for 72 h. The calculation of isotopologue of ribose is total intensity of 13 C-labeled ribose moiety. g U- 13 C glucose metabolic flux assays were performed in Plk1-overexpressing HeLa cells with G6PD knockdown by NMR. The results were normalized to cell numbers or cell wet weight. Data were represented as the mean ± s.d. or s.e.m. * P < 0.05 as compared to indicated group by two-sided Student’s t -test

Journal: Nature Communications

Article Title: Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway

doi: 10.1038/s41467-017-01647-5

Figure Lengend Snippet: Plk1 enhances PPP and biosynthesis in cancer cells. a Using [U- 13 C]-labeled glucose, M + 5-labeled R5P produced from PPP was detected by LC-MS. HeLa cells were synchronized by double HU block. When the cells were treated for the second round of HU block for 11 h, i.e., 1 h before releasing, U- 13 C glucose was added into the medium and further culture for 1 h and then harvested for G1 phase cell analysis. For cell samples of S and G2/M phases, after the regular second round of HU block for 12 h, cells were released into fresh HU-free medium and further cultured for 4 and 9 h, respectively, followed by supplementation of U- 13 C glucose and 1 h culture before harvesting cells for analysis. Cells were harvested and 13 C-incorporated R5P was detected by LC-MS. The calculation of % of the m + 5 isotopologue of ribose is % of the sum of isotopologues. b – f The PPP metabolites were detected by NMR. Cells were cultured in medium containing [2- 13 C]- or [U- 13 C]-labeled glucose. The graphic description shows that by using the [2- 13 C]-labeled glucose, NMR measurement could distinguish the lactate produced from PPP and that derived from the general glycolysis ( b ). Lactate derived from the oxidative PPP was calculated by the total lactate multiplied with the ratio of 3- 13 C lactate detected by NMR in Plk1 overexpression ( c ) or knockdown cells ( d ). Using the [U- 13 C]-labeled glucose, R5P and some downstream nucleotides generated from the PPP flux were detected by NMR in Plk1 overexpression ( e ) or knockdown cell lines ( f ). To induce Plk1 shRNA expression, cells were grown in the presence of 0.1 μg/ml doxycycline for 72 h. The calculation of isotopologue of ribose is total intensity of 13 C-labeled ribose moiety. g U- 13 C glucose metabolic flux assays were performed in Plk1-overexpressing HeLa cells with G6PD knockdown by NMR. The results were normalized to cell numbers or cell wet weight. Data were represented as the mean ± s.d. or s.e.m. * P < 0.05 as compared to indicated group by two-sided Student’s t -test

Article Snippet: NADP + , NADPH, GSH, G6P, 6PG, NAC, Dox, tetracycline, HU, propidium iodide, nocodazole, 4,6-diamidino-2-phenylindole (DAPI), casein, D 2 O, ribonucleosides, and deoxyribonucleosides were purchased from Sigma-Aldrich; R5P, nucleotides (AXP, GXP, CXP, and UXP, X = M, D, and T) were purchased from Sangon Biotech.

Techniques: Labeling, Produced, Liquid Chromatography with Mass Spectroscopy, Blocking Assay, Cell Culture, Derivative Assay, Over Expression, Generated, shRNA, Expressing