qubit fluorometry system broad range kit Search Results


cell metertm fluorimetric intracellular total ros activity assay kit  (AAT Bioquest)
90
AAT Bioquest cell metertm fluorimetric intracellular total ros activity assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Cell Metertm Fluorimetric Intracellular Total Ros Activity Assay Kit, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/fluorimetric+intracellular+total+ros+activity+assay+kit/pmc11212081-85-10-20
Average 90 stars, based on 1 article reviews
cell metertm fluorimetric intracellular total ros activity assay kit - by Bioz Stars, 2026-09
90/100 stars
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amplite fluorimetric d-lactate dehydrogenase assay kit  (AAT Bioquest)
90
AAT Bioquest amplite fluorimetric d-lactate dehydrogenase assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Amplite Fluorimetric D Lactate Dehydrogenase Assay Kit, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/amplitetm+colorimetric+l+lactate+assay+kit/pm37681955-215-51-57
Average 90 stars, based on 1 article reviews
amplite fluorimetric d-lactate dehydrogenase assay kit - by Bioz Stars, 2026-09
90/100 stars
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quant-it picogreen dsdna assay kit  (Promega)
90
Promega quant-it picogreen dsdna assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Quant It Picogreen Dsdna Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/quant+it+picogreen+dsdna+assay+kit/pmc10218745-148-20-25
Average 90 stars, based on 1 article reviews
quant-it picogreen dsdna assay kit - by Bioz Stars, 2026-09
90/100 stars
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ds dna hs assay kit  (Thermo Fisher)
99
Thermo Fisher ds dna hs assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Ds Dna Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/DNA/med_rxiv__2020__09__07__20183665-40-12-17
Average 99 stars, based on 1 article reviews
ds dna hs assay kit - by Bioz Stars, 2026-09
99/100 stars
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qubit dsdna assay kit  (Thermo Fisher)
99
Thermo Fisher qubit dsdna assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Qubit Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/Agarose/pmc03271783-410-7-11
Average 99 stars, based on 1 article reviews
qubit dsdna assay kit - by Bioz Stars, 2026-09
99/100 stars
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accublue broad range dsdna quantification kit  (Biotium)
93
Biotium accublue broad range dsdna quantification kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Accublue Broad Range Dsdna Quantification Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/AccuBlue+Broad+Range+dsDNA+Quantitation+Kit/pm24324616-75-13-19
Average 93 stars, based on 1 article reviews
accublue broad range dsdna quantification kit - by Bioz Stars, 2026-09
93/100 stars
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protein assay kit  (Thermo Fisher)
99
Thermo Fisher protein assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/BCA+Protein+Assay+Kit/10__3390_slash_pr8101228-89-15-18
Average 99 stars, based on 1 article reviews
protein assay kit - by Bioz Stars, 2026-09
99/100 stars
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quant it picogreen dsdna assay kit  (Molecular Devices LLC)
99
Molecular Devices LLC quant it picogreen dsdna assay kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Quant It Picogreen Dsdna Assay Kit, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/SpectraMax+i3x/pmc06426462-237-10-19
Average 99 stars, based on 1 article reviews
quant it picogreen dsdna assay kit - by Bioz Stars, 2026-09
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amplitetm fluorimetric calcium quantitation kit  (AAT Bioquest)
90
AAT Bioquest amplitetm fluorimetric calcium quantitation kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Amplitetm Fluorimetric Calcium Quantitation Kit, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/amplite+colorimetric+calcium+quantitation+kit/pm39033133-312-13-19
Average 90 stars, based on 1 article reviews
amplitetm fluorimetric calcium quantitation kit - by Bioz Stars, 2026-09
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sensolyte opa protein quantification kit fluorimetric  (AnaSpec)
90
AnaSpec sensolyte opa protein quantification kit fluorimetric
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Sensolyte Opa Protein Quantification Kit Fluorimetric, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/sensolyte+opa+protein+quantification+kit+fluorimetric/pmc11096370-387-25-31
Average 90 stars, based on 1 article reviews
sensolyte opa protein quantification kit fluorimetric - by Bioz Stars, 2026-09
90/100 stars
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flat transparent 96-well plate grenier  (Stonehouse Enterprises LLC)
90
Stonehouse Enterprises LLC flat transparent 96-well plate grenier
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Flat Transparent 96 Well Plate Grenier, supplied by Stonehouse Enterprises LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/flat+transparent+96+well+plate+grenier/pmc07074035-242-9-13
Average 90 stars, based on 1 article reviews
flat transparent 96-well plate grenier - by Bioz Stars, 2026-09
90/100 stars
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amplite fluorimetric glucose quantification kit  (AAT Bioquest)
90
AAT Bioquest amplite fluorimetric glucose quantification kit
Combination treatment with gefitinib and TRQ significantly increased <t>ROS</t> levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and <t>intracellular</t> ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.
Amplite Fluorimetric Glucose Quantification Kit, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qubit+fluorometry+system+broad+range+kit/amplite+fluorimetric+glucose+quantification+kit/pmc11864646-353-16-21
Average 90 stars, based on 1 article reviews
amplite fluorimetric glucose quantification kit - by Bioz Stars, 2026-09
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Image Search Results


Combination treatment with gefitinib and TRQ significantly increased ROS levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and intracellular ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.

Journal: Journal of Cancer

Article Title: Tanreqing injection inhibits stemness and enhances sensitivity of non-small cell lung cancer models to gefitinib through ROS/STAT3 signaling pathway

doi: 10.7150/jca.94438

Figure Lengend Snippet: Combination treatment with gefitinib and TRQ significantly increased ROS levels and cell redox ratio in NSCLC models. (A, B) Representative confocal image of mitochondrial superoxide (green, scale bars, 500 μm) and intracellular ROS (red, scale bars, 500 μm) in 2D cell lines after treated with gefitinib (2.5 μM), TRQ (0.6%) alone or together. (C, D) Representative confocal image of mitochondrial superoxide (green, scale bars, 50 μm) and intracellular ROS (yellow, scale bars, 50 μm) in 3D cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together. (E, F) Representative morphology image and composite image in cell spheres after treated with gefitinib (20 μM), TRQ (0.8%) alone or together (scale bars, 50 μm). The higher the value was, the lower the cell redox ratio (FAD/[NADH+FAD]) was. The results are represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; PC-9-PIK3CA-M, PC-9-PIK3CA-mutation.

Article Snippet: Intracellular total ROS was quantified following the instructions of by Cell MeterTM Fluorimetric Intracellular Total ROS Activity Assay Kit (22902, AAT Bioquest, CA, USA).

Techniques: Control, Injection, Mutagenesis

Combination treatment with gefitinib and TRQ inhibits the tumor growth in PC-9-PIK3CA-M xenograft model. (A) Evolution of tumor volumes of PC-9-PIK3CA-M xenograft model during treatment. (B) Representative PC-9-PIK3CA-M xenograft mice and tumors surgically removed. (C, D) The mRNA expression of SOX2, ALDH1A1 was assayed by PCR and protein levels of SOX2, ALDH1A1, p-STAT3 in tumors was detected by western blotting. (E) The intracellular ROS levels and mitochondrial superoxide levels in tumors was assayed by flow cytometry. Data were shown as means ± SD ( n = 5). * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; SOX2, sex determining region Y-box 2; ALDH1A1, aldehyde dehydrogenase family 1 member A1.

Journal: Journal of Cancer

Article Title: Tanreqing injection inhibits stemness and enhances sensitivity of non-small cell lung cancer models to gefitinib through ROS/STAT3 signaling pathway

doi: 10.7150/jca.94438

Figure Lengend Snippet: Combination treatment with gefitinib and TRQ inhibits the tumor growth in PC-9-PIK3CA-M xenograft model. (A) Evolution of tumor volumes of PC-9-PIK3CA-M xenograft model during treatment. (B) Representative PC-9-PIK3CA-M xenograft mice and tumors surgically removed. (C, D) The mRNA expression of SOX2, ALDH1A1 was assayed by PCR and protein levels of SOX2, ALDH1A1, p-STAT3 in tumors was detected by western blotting. (E) The intracellular ROS levels and mitochondrial superoxide levels in tumors was assayed by flow cytometry. Data were shown as means ± SD ( n = 5). * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; SOX2, sex determining region Y-box 2; ALDH1A1, aldehyde dehydrogenase family 1 member A1.

Article Snippet: Intracellular total ROS was quantified following the instructions of by Cell MeterTM Fluorimetric Intracellular Total ROS Activity Assay Kit (22902, AAT Bioquest, CA, USA).

Techniques: Expressing, Western Blot, Flow Cytometry, Control, Injection

Combination treatment with gefitinib and TRQ inhibits the organoids growth. (A) Seven organoids were treated with TRQ or gefitinib or their combination for 72 h, CellTiter-Glo® 3D Cell Viability Assay was performed to detect the cell viability ( n = 7). (B) The Q value method was used to assess the combined effect in seven organoids. The Q values were calculated from the average inhibition rate of each group. (C) The mRNA expression of SOX2 in organoids was assayed by PCR ( n = 3). (D) Representative morphology image and composite image in organoids (scale bars, 50 μm) ( n = 4). (E) Representative image of intracellular ROS (red, scale bars, 100 μm) and mitochondrial superoxide (green, scale bars, 100 μm) in organoids ( n = 3). The results were represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; SOX2, sex determining region Y-box 2.

Journal: Journal of Cancer

Article Title: Tanreqing injection inhibits stemness and enhances sensitivity of non-small cell lung cancer models to gefitinib through ROS/STAT3 signaling pathway

doi: 10.7150/jca.94438

Figure Lengend Snippet: Combination treatment with gefitinib and TRQ inhibits the organoids growth. (A) Seven organoids were treated with TRQ or gefitinib or their combination for 72 h, CellTiter-Glo® 3D Cell Viability Assay was performed to detect the cell viability ( n = 7). (B) The Q value method was used to assess the combined effect in seven organoids. The Q values were calculated from the average inhibition rate of each group. (C) The mRNA expression of SOX2 in organoids was assayed by PCR ( n = 3). (D) Representative morphology image and composite image in organoids (scale bars, 50 μm) ( n = 4). (E) Representative image of intracellular ROS (red, scale bars, 100 μm) and mitochondrial superoxide (green, scale bars, 100 μm) in organoids ( n = 3). The results were represented as mean ± SD. * P < 0.05 versus the control group, # P < 0.05 versus the gefitinib alone group, ** P or ## P < 0.01, *** P or ### P < 0.001. Ctrl, the control group; TRQ, Tanreqing injection; Gefi, gefitinib; SOX2, sex determining region Y-box 2.

Article Snippet: Intracellular total ROS was quantified following the instructions of by Cell MeterTM Fluorimetric Intracellular Total ROS Activity Assay Kit (22902, AAT Bioquest, CA, USA).

Techniques: Viability Assay, Inhibition, Expressing, Control, Injection