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Image Search Results
Journal: iScience
Article Title: BTLA and PD-1 signals attenuate TCR-mediated transcriptomic changes
doi: 10.1016/j.isci.2024.110253
Figure Lengend Snippet:
Article Snippet:
Techniques: Gene Expression, Recombinant, Retroviral, Expressing, Plasmid Preparation, Software
Journal: Cell reports
Article Title: Antigen-Presenting Cell-Intrinsic PD-1 Neutralizes PD-L1 in cis to Attenuate PD-1 Signaling in T Cells
doi: 10.1016/j.celrep.2018.06.054
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Cell surface expression of PD-1 and PD-L1 on double positive cells were quantified using the Quantum APC MESF kit and
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, Software, Imaging
Journal: JCI Insight
Article Title: Linking adipose tissue eosinophils, IL-4, and leptin in human obesity and insulin resistance
doi: 10.1172/jci.insight.170772
Figure Lengend Snippet: ( A ) mRNA expression of eotaxin 1, 2, and 3 (CCL11, CCL24, and CCL26, respectively) is decreased in s.c. AT from individuals with BMI ≥ 30 kg/m 2 . n = 12 for both groups. ( B ) MESF of CCR3 is decreased in patients with BMI ≥ 30 kg/m 2 . Results were equivalent using either EMR1 or SIGLEC8 as an EOS-specific cell surface marker. BMI < 25 kg/m 2 , n = 15; BMI ≥ 30 kg/m 2 , n = 13. ( C ) CCR3 mRNA expression is decreased in EOS from individuals with BMI ≥ 30 kg/m 2 , mirroring MESF quantification of CCR3 cell surface protein expression. n = 11 for both groups. Eotaxin 1, CCL11 ; eotaxin 2, CCL24 ; eotaxin 3, CCL26 ; MESF, molecules of equivalent soluble fluorophores; EMR1, EGF-like module containing mucin-like hormone receptor 1; SIGLEC8, sialic-acid-binding immunoglobulin-like lectin−8. ( A and C ) the y axes indicate relative fold change (2 –ΔΔCt ). Data were analyzed by unpaired 2-tailed t test and expressed as mean ± SEM.
Article Snippet: Fluorescence intensity was calibrated using
Techniques: Expressing, Marker, Binding Assay