Journal: Journal of Inflammation (London, England)

Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics

doi: 10.1186/s12950-025-00463-9

Figure Lengend Snippet: Alcohol + CI administration aggravated pancreatic histopathological damage and reduced HIF-1α methylation in alcoholic AP rats. A HE staining was applied to assess pancreatic pathology injuries at various dosages of CI in rats (magnification 200×, scale bar: 100 μm), n = 3. B ELISA assay was performed for serum expression of HIF-1α and VEGF, n = 6. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; HE: Hematoxylin and Eosin; ELISA: Enzyme-Linked Immunosorbent Assay; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGF: Vascular Endothelial Growth Factor

Article Snippet: As described previously [ ], on the basis of base-specific cleavage and the time of flight mass spectrometry (MALDI-TOF MS), quantitative methylation detection of the HIF-1α gene was performed using Sequenom MassARRAY platform (Agena Bioscience) according to the manufacturer’s protocol.

Techniques: Methylation, Staining, Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: Journal of Inflammation (London, England)

Article Title: In-depth investigation of the mechanism in rats with alcoholic acute pancreatitis via DNA methylation, intestinal flora, and fecal metabolomics

doi: 10.1186/s12950-025-00463-9

Figure Lengend Snippet: Alcohol + CI exacerbated hepatic and pancreatic injury and inflammatory response through HIF-1α methylation in alcoholic AP rats. A , B The serum content of lipase and amylase was examined by ELISA assay, n = 6; C - H Biochemical analysis was performed to measure the serum levels of AST, ALT, GGT, BUN, ALP, and CREA, n = 6; I Western blot analysis for TNF-α, IL-6, and IL-1β expression in pancreas of rats was made, n = 3; J MSP was used to evaluate HIF-1α methylation in pancreas of rats, n = 3; K Western blot analysis was employed to assess the expression of mTOR, p-mTOR, HIF-1α, and VEGF in pancreas of rats, n = 3. ▲ P < 0.05 and ▲▲ P < 0.01 vs. Control group; ★ P < 0.05 and ★★ P < 0.01 vs. Alcohol group. Note: AP: Acute Pancreatitis; CI: Cerulein; AST: Aspartate Aminotransferase; ALT: Alanine Aminotransferase; GGT: Gamma-Glutamyl Transferase; BUN: Blood Urea Nitrogen; ALP: Alkaline Phosphatase; CREA: Creatinine; TNF-α: Tumor Necrosis Factor-alpha; IL-6: Interleukin 6; IL-1β: Interleukin 1 beta; MSP: Methylation-Specific PCR (Polymerase Chain Reaction); mTOR: Mammalian Target of Rapamycin; p-Mtor: Phosphorylated Mammalian Target of Rapamycin; HIF-1α: Hypoxia-Inducible Factor 1 Alpha, VEGF: Vascular Endothelial Growth Factor

Article Snippet: As described previously [ ], on the basis of base-specific cleavage and the time of flight mass spectrometry (MALDI-TOF MS), quantitative methylation detection of the HIF-1α gene was performed using Sequenom MassARRAY platform (Agena Bioscience) according to the manufacturer’s protocol.

Techniques: Methylation, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control, Polymerase Chain Reaction