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Image Search Results
Journal: BMC Cancer
Article Title: Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer
doi: 10.1186/s12885-018-4528-x
Figure Lengend Snippet: Correlation of ERRβ expression with ERα in breast tumors and cell lines. a Immunohistochemical staining with ERRβ antibody in ER + ve and ER-ve breast cancer patients. Elevated expression of ERRβ was found in ER + ve ( n = 6) compared to ER-ve ( n = 6) breast cancer patient samples. b Graphical representation of IHC composite scores of each tissue microarray sample showing significant elevated expression of ERRβ in ER + ve than in ER-ve breast cancer patient samples. Graph was plotted using composite score and p -values were calculated using 2-group t-test ( p < 0.05 considered as significant). c , d Western blots, Reverse transcription polymerase chain reaction (RT PCR) and densitometry analysis results representing elevated levels of ERRβ in ER + ve breast cancer cells (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Statistical significance for relative gene expression (RT PCR) and normalized percentage of expression (WB) was analyzed using One-way ANOVA and unpaired t-test respectively ( p -value < 0.05 was considered as significant)
Article Snippet: The
Techniques: Expressing, Immunohistochemical staining, Staining, Microarray, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Gene Expression
Journal: BMC Cancer
Article Title: Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer
doi: 10.1186/s12885-018-4528-x
Figure Lengend Snippet: Expression of ERRβ is ERα dependent. Efficient knockdown of ERα showing significant decrease in the expression of ERRβ in MCF7 cells. a , b Quantitative Real-time PCR (qRT-PCR) and Reverse transcription polymerase chain reaction (RT-PCR) results showing decreased expression of ERRβ in ERα depleted MCF7 cells. Housekeeping gene GAPDH treated as control and ΔCt, ΔΔCt, 2 -ΔΔCt values were calculated and graph was plotted using 2 -ΔΔCt values. Fold change ≥ 2 was considered as significant. p -values were calculated using 2-group t-test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). c Western blot revealing the depleted expression of ERRβ in ERα Knockdown MCF7 (ERα KD) cells
Article Snippet: The
Techniques: Expressing, Knockdown, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Control, Western Blot