px458 sgrna Search Results


cells with px458 2a gfp sgrna trim25 g1  (Addgene inc)
91
Addgene inc cells with px458 2a gfp sgrna trim25 g1
Cells With Px458 2a Gfp Sgrna Trim25 G1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px458 sgrna  (Addgene inc)
90
Addgene inc px458 sgrna
Px458 Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek293t cells  (Addgene inc)
93
Addgene inc hek293t cells
Hek293t Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px458 sgrna ago1 2  (Addgene inc)
90
Addgene inc px458 sgrna ago1 2
Immunoblot analysis of DROSHA, DICER, <t>AGO1,</t> and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.
Px458 Sgrna Ago1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px458 sgrna ago1 2/product/Addgene inc
Average 90 stars, based on 1 article reviews
px458 sgrna ago1 2 - by Bioz Stars, 2026-03
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px458 vector  (Addgene inc)
90
Addgene inc px458 vector
Immunoblot analysis of DROSHA, DICER, <t>AGO1,</t> and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.
Px458 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px458 vector/product/Addgene inc
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pspcas9 bb 2a gfp px458 vector  (Addgene inc)
91
Addgene inc pspcas9 bb 2a gfp px458 vector
Immunoblot analysis of DROSHA, DICER, <t>AGO1,</t> and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.
Pspcas9 Bb 2a Gfp Px458 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids 108 sypgcamp  (Addgene inc)
90
Addgene inc plasmids 108 sypgcamp
Immunoblot analysis of DROSHA, DICER, <t>AGO1,</t> and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.
Plasmids 108 Sypgcamp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmids 108 sypgcamp/product/Addgene inc
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px458 sgrna ago2 4  (Addgene inc)
90
Addgene inc px458 sgrna ago2 4
Immunoblot analysis of DROSHA, DICER, <t>AGO1,</t> and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.
Px458 Sgrna Ago2 4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/px458 sgrna ago2 4/product/Addgene inc
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109 maj106 109 dh5α harboring adh expression plasmids p32 p35  (Addgene inc)
91
Addgene inc 109 maj106 109 dh5α harboring adh expression plasmids p32 p35
Immunoblot analysis of DROSHA, DICER, <t>AGO1,</t> and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.
109 Maj106 109 Dh5α Harboring Adh Expression Plasmids P32 P35, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/109 maj106 109 dh5α harboring adh expression plasmids p32 p35/product/Addgene inc
Average 91 stars, based on 1 article reviews
109 maj106 109 dh5α harboring adh expression plasmids p32 p35 - by Bioz Stars, 2026-03
91/100 stars
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Image Search Results


Immunoblot analysis of DROSHA, DICER, AGO1, and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.

Journal: EMBO Reports

Article Title: Global and precise identification of functional miRNA targets in mESCs by integrative analysis

doi: 10.15252/embr.202254762

Figure Lengend Snippet: Immunoblot analysis of DROSHA, DICER, AGO1, and AGO2 in WT and their respective KO mESC lines. TUBULIN was used as a loading control. Representative blot of three independent experiments is shown. PCA of gene expression as measured by RNA sequencing in miRNA _KO and WT samples. Biological replicates are indicated with corresponding colors. MA plots of the DGE analysis in miRNA _KO mutants versus WT. Significant up‐ and downregulated genes are colored in red and blue, respectively. Overlap of up‐ (left) and downregulated (right) genes in miRNA _KO mESCs. Comparison of miRNA loading for small RNA sequencing (Input) versus Argonaute2 & 1 (AGOs) RNA immunoprecipitation (RIP) and sequencing for all miRNAs. MiRNAs that show statistically significant difference between the two measured (adjusted P ‐value < 0.1) are highlighted. Data information: In (C and E), significance in differential analysis was determined using an adjusted P ‐value threshold obtained from DESeq2 of 0.1. In (D), an adjusted P ‐value threshold of 0.2 was applied. Source data are available online for this figure.

Article Snippet: pX458‐sgRNA_ Ago1 _2 , Addgene , #73534.

Techniques: Western Blot, Control, Gene Expression, RNA Sequencing, Comparison, RNA Immunoprecipitation, Sequencing

Journal: EMBO Reports

Article Title: Global and precise identification of functional miRNA targets in mESCs by integrative analysis

doi: 10.15252/embr.202254762

Figure Lengend Snippet:

Article Snippet: pX458‐sgRNA_ Ago1 _2 , Addgene , #73534.

Techniques: Recombinant