px330-grna vector Search Results


99
New England Biolabs cas9 expression vector
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Addgene inc chimeric guide rna expression plasmid px330
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93
Addgene inc grna cloning vector px330
Grna Cloning Vector Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc grnas into px330
Grnas Into Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Addgene inc grna oligos
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Addgene inc crispr cas9 grna expression plasmid px330
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Addgene inc cas9 guide rna expression plasmid px330
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96
Addgene inc empty crispri plasmid
KEY RESOURCES TABLE
Empty Crispri Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Broad Institute Inc px330-u6-chimeric_bb-cbh-hspcas9
KEY RESOURCES TABLE
Px330 U6 Chimeric Bb Cbh Hspcas9, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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KEY RESOURCES TABLE

Journal: Neuron

Article Title: Mutations in spliceosomal genes PPIL1 and PRP17 cause neurodegenerative pontocerebellar hypoplasia with microcephaly

doi: 10.1016/j.neuron.2020.10.035

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Empty CRISPRi plasmid (PX330-U6–2XBsmBI-gRNA-CBh-dCas9-KRAB-T2a-Puro) was generated on the modified PX330 with 2× BsmBI gRNA cloning sites. dCas9-KRAB-T2a-Puro was amplified from vector pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2aPuro (Addgene #71236) and cloned inside PX330 to replace original WT Cas9. gRNAs targeting PRP17 or scramble gRNA was further cloned between 2XBsmBI sites.

Techniques: Virus, Recombinant, Flow Cytometry, cDNA Synthesis, RNAscope, Multiplex Assay, Control, Knock-Out, Knock-In, Mutagenesis, Plasmid Preparation, Software