px330 Search Results


px330  (Addgene inc)
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Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pten grna1  (Addgene inc)
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plasmid mixture  (Addgene inc)
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px330 u6 chimeric bb cbh hspcas9 plasmid  (Addgene inc)
96
Addgene inc px330 u6 chimeric bb cbh hspcas9 plasmid
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Px330 U6 Chimeric Bb Cbh Hspcas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px330 mcherry plasmid  (Addgene inc)
95
Addgene inc px330 mcherry plasmid
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Px330 Mcherry Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aavs1 t2 crispr in px330  (Addgene inc)
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Aavs1 T2 Crispr In Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px330 u6 chimeric bb cbh hspcas9 hgem  (Addgene inc)
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plasmid px330 mcherry rosa26 sg  (Addgene inc)
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paper n a px330 tp53  (Addgene inc)
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px330 gfp sfpq  (Addgene inc)
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px330 tp53  (Addgene inc)
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px330 en1082 rad21 stop  (Addgene inc)
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Journal: Neuron

Article Title: Mutations in spliceosomal genes PPIL1 and PRP17 cause neurodegenerative pontocerebellar hypoplasia with microcephaly

doi: 10.1016/j.neuron.2020.10.035

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Empty CRISPRi plasmid (PX330-U6–2XBsmBI-gRNA-CBh-dCas9-KRAB-T2a-Puro) was generated on the modified PX330 with 2× BsmBI gRNA cloning sites. dCas9-KRAB-T2a-Puro was amplified from vector pLV-hU6-sgRNA-hUbC-dCas9-KRAB-T2aPuro (Addgene #71236) and cloned inside PX330 to replace original WT Cas9. gRNAs targeting PRP17 or scramble gRNA was further cloned between 2XBsmBI sites.

Techniques: Virus, Recombinant, Flow Cytometry, cDNA Synthesis, RNAscope, Multiplex Assay, Control, Knock-Out, Knock-In, Mutagenesis, Plasmid Preparation, Software