Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: PVT1 was highly expressed in the kidneys of VAN-induced AKI mice. (A)–(E) Mice were intraperitoneally injected with VAN (600 mg kg −1 ) for the indicated time points (0, 1, 3, 7 day). (A) and (B) BUN and Scr levels were determined by respective commercial kits. (C) Histopathological alterations of kidney tissues were monitored by H&E staining. (D) TUNEL assay was performed to detect cell apoptosis pattern in kidney tissues. (E) Western blot assay was conducted to detect protein levels of Bax and Bcl-2 in kidney tissues. (F) PVT1 level in kidney tissues was measured by RT-qPCR assay. * P < 0.05.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: Injection, Staining, TUNEL Assay, Western Blot, Quantitative RT-PCR

Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: PVT1 knockdown ameliorated VAN-induced AKI in vivo . (A)–(E) Mice were injected with si-NC or si-PVT1 for 24 h and then intraperitoneally injected with VAN (600 mg kg −1 ) for 7 days. (A) PVT1 level in kidney tissues were measured by RT-qPCR assay. (B) and (C) BUN and Scr levels were determined by matching commercial kits. (D) Histopathological changes of kidney tissues were detected by H&E staining. (E) Cell apoptosis in kidney tissues was tested by TUNEL assay. (F) Western blot assay was conducted to detect protein levels of Bax and Bcl-2 in kidney tissues. * P < 0.05.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: Knockdown, In Vivo, Injection, Quantitative RT-PCR, Staining, TUNEL Assay, Western Blot

Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: PVT1 directly interacted with miR-124. (A) Predicted binding sites between PVT1 and miR-124 by miRcode online website and mutant sites in PVT1-MUT reporter. (B) 293T cells were co-transfected with miR-NC or miR-124 and PVT1-WT reporter or PVT1-MUT reporter, followed by the determination of luciferase activities at 48 h after transfection. (C) RIP and RT-qPCR assays were carried out to measure the enrichment levels of PVT1 and miR-124 in IgG or Ago2 immunoprecipitation complex in HK-2 cells. (D) RNA pull down and RT-qPCR assays were performed to investigate whether miR-124 could interact with PVT1 by predicted binding sites in HK-2 cells. The experiments were repeated at least three times. * P < 0.05.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: Binding Assay, Mutagenesis, Transfection, Luciferase, Quantitative RT-PCR, Immunoprecipitation

Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: The silencing of PVT1 abated VAN-induced HK-2 cell apoptosis in vitro . (A)–(C) HK-2 cells were stimulated with 4 mM VAN for 24 h. (A) PVT1 level was measured by RT-qPCR assay. (B) Apoptosis index was determined by double-staining of Annexin V-FITC and PI using a flow cytometry. (C) Protein levels of Bcl-2 and Bax were detected by western blot assay. (D)–(F) HK-2 cells were transfected with si-NC or si-PVT1 for 24 h and then stimulated with 4 mM VAN for additional 24 h, followed by the determination of PVT1 (D), Bcl-2 (F) and Bax (F) levels, and apoptosis index (E). The experiments were repeated at least three times. * P < 0.05.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: In Vitro, Quantitative RT-PCR, Double Staining, Flow Cytometry, Western Blot, Transfection

Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: The inhibition of miR-124 abrogated si-PVT1-mediated anti-apoptosis effect in VAN-treated HK-2 cells. (A) HK-2 cells were stimulated with 4 mM VAN for 24 h, followed by the measurement of miR-124 level via RT-qPCR assay. (B)–(D) HK-2 cells were transfected with si-NC, si-PVT1, si-PVT1 + anti-miR-NC or si-PVT1 + anti-miR-124 for 24 h and then treated with 4 mM VAN for 24 h followed by the measurement of miR-124 (B), Bcl-2 (D) and Bax (D) levels, and apoptosis index (C). The experiments were repeated at least three times. * P < 0.05.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: Inhibition, Quantitative RT-PCR, Transfection

Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: PVT1 activated NF-κB signaling by targeting miR-124 in VAN-induced HK-2 cells. (A) and (B) HK-2 cells were transfected with pcDNA, PVT1, PVT1 + miR-NC, PVT1 + miR-124, si-NC, si-PVT1, si-PVT1 + anti-miR-NC, or si-PVT1 + anti-miR-124 for 24 h and then treated with 4 mM VAN for 24 h. Then, protein levels of p-p65, p65, p-IκBα and IκBα were determined by western blot assay. The experiments were repeated at least three times.* P < 0.05.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: Transfection, Western Blot

Journal: RSC Advances

Article Title: Retracted Article: PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivation of NF-κB signaling

doi: 10.1039/c8ra05724a

Figure Lengend Snippet: The schematic diagram of PVT1 knockdown alleviates vancomycin-induced acute kidney injury by targeting miR-124 via inactivating NF-κB signaling.

Article Snippet: Then, the relative expression of PVT1 (mouse or human) was measured using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) for PVT1 (Hs01069023_m1 or Mm01203908_m1) and GAPDH (Hs00266705_g1 or Mm99999915_g1) with GAPDH as the endogenous control.

Techniques: Knockdown

Journal: PLoS Genetics

Article Title: A Functional Variant at a Prostate Cancer Predisposition Locus at 8q24 Is Associated with PVT1 Expression

doi: 10.1371/journal.pgen.1002165

Figure Lengend Snippet: P-values are for univariate linear regression analysis of PVT1 expression in relation to 0, 1 and 2 risk allele (G) of rs378854, adjusted for race. Expression values are shown on log2 scale relative to a mean value of all samples. Expression is lowest in carriers of non-risk AA genotypes and highest in carriers of risk GG genotypes. Mean expression values of each group are shown as bars.

Article Snippet: Expression of alternative splicing forms of PVT1 measured by assay Hs01069044 was beyond confident detection level even with 20 ng of total RNA and was not used on individual tissue samples.

Techniques: Expressing

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Test results of Hardy-Weinberg Equilibrium among all PVT1 gene sites.

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Control

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Association between lncRNA PVT1 polymorphisms and EH risk in the south of China.

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Control

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Association between lncRNA PVT1 polymorphisms and EH risk in the north of China.

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Control

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Association between lncRNA PVT1 polymorphisms and EH risk in China.

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Control

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Influence of PVT1 rs10956390 polymorphism on lipid level of subjects: (a) TG; (b) TC; (c) HDL-C; (d) LDL-C. (Data are expressed as the mean ± SEM. Control: CC, n = 108, CT, n = 220, TT, n = 111; EH: CC, n = 192, CT, n = 233, TT, n = 99. ∗ P < 0.05, ∗∗ P < 0.01, as compared with CC genotype in interclass; # P < 0.05, as compared with CT genotype in interclass).

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Control

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Influence of PVT1 rs80177647 polymorphism on lipid level of subjects: (a) TG; (b) TC; (c) HDL-C; (d) LDL-C. (Data are expressed as the mean ± SEM. Control: TT, n = 377, TA, n = 57, AA, n = 5; EH: TT, n = 395, TA, n = 119, AA, n = 10.).

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Control

Journal: BioMed Research International

Article Title: Association of lncRNA PVT1 Gene Polymorphisms with the Risk of Essential Hypertension in Chinese Population

doi: 10.1155/2022/9976909

Figure Lengend Snippet: Effect of PVT1 mRNA expression in whole blood from healthy normal donors: (a) rs10956390; (b) rs80177647.

Article Snippet: Assay ID for rs10956390 is C__317048_10 (Thermo), and assay ID for rs80177647 is C__101092625_10 (Thermo).

Techniques: Expressing

Journal: Biomolecules

Article Title: Transcriptome Analyses Identify Deregulated MYC in Early Onset Colorectal Cancer

doi: 10.3390/biom12091223

Figure Lengend Snippet: MYC and PVT1 copy number alterations are found in some patient tumors. ( A ) Bar graph depicting the copy number of the MYC gene locus in patient-matched uninvolved colonic segments (normal, N) and tumors (T). Highlighted in blue are tumor samples with 2.5 or greater copy number. ( B ) Correlation of MYC copy number and MYC expression in tumors. Red points are tumors expressing high- MYC and blue points are tumors expressing low- MYC (Pearson correlation estimate 0.68, p = 5.45 × 10 −6 ). ( C ) Bar graph depicting the copy number of the PVT1 locus in the six patient-matched samples displaying gains in the MYC genomic locus in tumors (T). Uninvolved colonic tissues (normal, N) are shown as a reference. In ( A , C ), highlighted in blue are tumors samples with 2.5 or greater increases in copy number. Error bars represent the range of copy numbers detected in four technical replicates per sample.

Article Snippet: The MYC genomic locus was evaluated using the Taqman probe Hs02602824_cn (Thermo Fisher), and the PVT1 genomic locus was evaluated using Taqman probe Hs06232749_cn.

Techniques: Expressing

Journal: Biomolecules

Article Title: Transcriptome Analyses Identify Deregulated MYC in Early Onset Colorectal Cancer

doi: 10.3390/biom12091223

Figure Lengend Snippet: Expression of PVT1 correlates with MYC expression. ( A ) rlog normalized expression plot of PVT1 in patient-matched adjacent colonic tissues (Normal) and tumors (paired Wilcoxon test, p = 4.8 × 10 −6 ). Each point represents a single patient with a line drawn between matched samples. ( B ) Correlation plot depicting normalized MYC versus normalized PVT1 gene expression in adjacent colonic segments (Normal, blue points) and tumors (red points). ( C ) Correlation plot depicting PVT1 and MYC differential expression in patient-matched adjacent colonic segments and tumors.

Article Snippet: The MYC genomic locus was evaluated using the Taqman probe Hs02602824_cn (Thermo Fisher), and the PVT1 genomic locus was evaluated using Taqman probe Hs06232749_cn.

Techniques: Expressing, Gene Expression, Quantitative Proteomics

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: The lncRNA PVT1 Is Overexpressed in Cholangiocarcinoma Tissues (A) Hierarchical clustering analysis of lncRNAs that were differentially expressed (fold change > 2; p < 0.05) in cholangiocarcinoma tissues and normal tissues. (B) Overlap of dysregulated lncRNAs in GEO datasets. (C) PVT1 was detected in 17 pairs of CCA tissues by qRT-PCR. The levels of PVT1 in CCA tissues were significantly higher than those in non-tumorous tissues.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: Quantitative RT-PCR

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: PVT1 Promotes Cell Proliferation and Migration in Cholangiocarcinoma Cells (A) qRT-PCR was used to determine the expression of PVT1 after siRNA transfection in the HuCCT1 and RBE cell lines. (B) Colony formation assays were used to determine the colony-forming ability of si- PVT1 -transfected cells. (C) CCK-8 assays were used to determine the viability of si- PVT1 -transfected cholangiocarcinoma cells. (D) Transwell assays showed that PVT1 knockdown inhibited cholangiocarcinoma cell migration. The error bars indicate the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: Migration, Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: Knockdown of PVT1 Causes Apoptosis by Promoting Cell-Cycle Arrest In Vitro (A) Fluorescence-activated cell sorting (FACS) analysis of the effect of PVT1 on apoptosis. (B) FACS analysis of the effect of PVT1 on cell cycle progression. The error bars indicate the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: In Vitro, Fluorescence, FACS

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: PVT1 Regulates CCA Cell Proliferation In Vivo (A) Tumors established with the mice in the scrambled control or sh-PVT1 group. (B) The size of tumors in the scrambled control or sh-PVT1 group. (C) After cell injection, the tumor volumes were calculated every 4 days. (D) The tumor weights are presented as the means ± SD. The error bars indicate the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: In Vivo, Injection

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: RNA-Seq after PVT1 Knockdown in HuCCT1 Cells (A) Mean-centered hierarchical clustering analysis of 1,295 transcripts that were altered (≥1.5-fold change) in si-NC-treated cells and siRNA- PVT1 -treated cells, assessed in triplicate. (B) Gene ontology analysis for all genes with altered expression. (C and D) The mRNA levels of the altered genes were selectively confirmed by qRT-PCR in PVT1 -knockdown HuCCT1 (C) and RBE (D) cells. (E) The mRNA levels of the altered genes were selectively confirmed by qRT-PCR in PVT1 -overexpressing cells. The error bars indicate the means ± SD. *p < 0.05, **p < 0.01; ns, not significant.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: RNA Sequencing Assay, Expressing, Quantitative RT-PCR

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: PVT1 Binds with EZH2 to Coregulate Target Genes, Especially ANGPTL4 (A) After nuclear and cytosolic separation, RNA expression levels were measured by qRT-PCR. GAPDH was used as a cytosolic marker and U1 was used as a nuclear marker. (B) Fluorescent images of RBE cells treated with anti- PVT1 (red), anti- 18S (red), and anti- U6 (red) RNA probes. DAPI staining is shown in blue. (C) The probability of interaction of EZH2 and PVT1 was determined with an online tool ( http://pridb.gdcb.iastate.edu/RPISeq/index.html ). Predictions with probabilities >0.5 were considered positive. RPI-seq predictions are based on random forest (RF) or support vector machine (SVM). (D) An RIP experiment for EZH2 was performed, and the coprecipitated RNA was subjected to qRT-PCR for PVT1 . (E) Expression and co-localization of EZH2 and PVT1 in HuCCT1 cells. Representative fluorescent images show HuCCT1 cells treated with fluorescently labeled anti-EZH2 antibody (green) and anti- PVT1 RNA (red). DAPI staining indicates the cell nuclei (blue). (F) Methylation-related genes were detected by qRT-PCR in the HuCCT1 and RBE cell lines after knockdown of EZH2 . (G) Methylation-related genes were detected by qRT-PCR in the HuCCT1 and RBE cell lines after overexpression of EZH2 . (H) The correlation between EZH2 and ANGPTL4 expression was detected by analyzing GEO: GSE26566 data. (I) The altered protein levels of ANGPTL4 were selectively confirmed by western blot analysis in cells with knockdown of PVT1 or EZH2 . (J) The altered protein levels of ANGPTL4 were selectively confirmed by western blotting in cells overexpressing PVT1 or EZH2 . The error bars indicate the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: RNA Expression, Quantitative RT-PCR, Marker, Staining, Plasmid Preparation, Expressing, Labeling, Methylation, Over Expression, Western Blot

Journal: Molecular Therapy. Nucleic Acids

Article Title: Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

doi: 10.1016/j.omtn.2018.10.001

Figure Lengend Snippet: PVT1 Binds with EZH2 in the Nucleus and Epigenetically Silences ANGPTL4 , Inhibiting Cell Proliferation and Migration in CCA Cell Lines (A) Expression level of ANGPTL4 in cholangiocarcinoma based on the analysis of GEO: GSE26566 data. (B) ANGPTL4 expression was determined in 17 pairs of CCA tissues by qRT-PCR. (C–F) HuCCT1 and RBE cells transfected with vector/ ANGPTL4 /pcDNA- PVT1 and cells transfected with PVT1 followed by transfection with ANGPTL4 . After transfection, the cells were analyzed by CCK-8 assays (C and D) and transwell assays (E and F). (G and H) ChIP of EZH2 and H3K27me3 of the promoter region of the ANGPTL4 locus after siRNA treatment with si-NC and si- PVT1 2# (G) or overexpression of PVT1 (H) in HuCCT1 cells. qPCR was performed to quantify the ChIP assay products. Enrichment was quantified relative to the input controls. Antibodies directed against IgG were used as a negative control. (I) Proposed model by which PVT1 regulates ANGPTL4 expression to promote CCA tumor growth. The error bars indicate the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.

Article Snippet: After rehydration, in situ hybridization was performed with a fluorescein-labeled PVT1 probe (RiboBio, Guangzhou, China), followed by reaction with a goat anti-EZH2 antibody (Proteintech, Wuhan, China) at 4°C overnight.

Techniques: Migration, Expressing, Quantitative RT-PCR, Transfection, Plasmid Preparation, CCK-8 Assay, Over Expression, Negative Control