Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD112 , REA1195 , 50 , 130-122-770 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Cellular neighborhood analysis of PD1 high/low T cells in the tumor margin and core. (A–D) Topology of PD1 high (left) and PD1 low (right) T cells and their cellular neighborhood within a 5 µm range. (A, B) represent tumor margin and (C, D) show tumor core areas. Cell types showing different distribution patterns around PD1 high and PD1 low T cells (mDCs, M1-like M, M2-like M, MDSCs, Fibroblasts, vessels, tumor cells) are highlighted by arrowheads. (E, F) Quantification of cells in a 5 µm range around of PD1 high/low T cells for tumor margin and tumor core, (E) represents immune cells and (F) stroma/tumor cells. (G) Violin plots for expression levels of eight immune-modulating markers (CD112, CD155, CD276, CD39, CD73, IDO, PD-L1, and VISTA) for the most important immune and tumor cells around PD1 high/low T cells in the tumor core area. Violin plots for tumor margin are shown in Supplementary Figure S5E . Depicted markers and annotated cell types as indicated by the color code. ROI sizes: Tumor margin (ROI15) and tumor core (ROI16): 975 x 769 µm.

Article Snippet: CD112 , REA1195 , 50 , 130-122-770 , PE , Miltenyi Biotec.

Techniques: Expressing

Journal: Cancers

Article Title: Targeting High-Risk Neuroblastoma Patient-Derived Xenografts with Oncolytic Virotherapy

doi: 10.3390/cancers14030762

Figure Lengend Snippet: Neuroblastoma PDXs express viral entry receptors. ( A ) Flow cytometry was used to detect the cell surface expression of the four main HSV receptors: CD111, CD112, syndecan, and HVEM in COA3, COA6, and COA129 human neuroblastoma PDX cells. All PDXs expressed all four receptors. ( B ) Representative histograms of CD111, CD112, syndecan, and HVEM cell surface expression and negative controls of COA6 are shown. Data reported as mean ± SEM and represent at least three biologic replicates.

Article Snippet: After 15 min, 10 μL of phycoerythrin (PE) conjugated anti-human CD111 antibody (Miltenyi Biotec), PE anti-human CD112 antibody (Miltenyi Biotec), allophycocyanin (APC) conjugated anti-human syndecan (Miltenyi Biotec), or APC anti-human HVEM (Miltenyi Biotec), were added for 20 min on ice in the dark.

Techniques: Flow Cytometry, Expressing

Journal: Cell Death & Disease

Article Title: Deciphering the tumor immune microenvironment of imatinib-resistance in advanced gastrointestinal stromal tumors at single-cell resolution

doi: 10.1038/s41419-024-06571-3

Figure Lengend Snippet: a Cell communication analysis on TIGIT-NECTIN2 and BTLA-TNFRSF14 pair between different cell types in imatinib resistant and sensitive patients respectively. Expression of NECTIN2 ( b ), BTLA ( c ) and TNFRSF14 ( d ) in each cell type in imatinib resistant and sensitive patients respectively. e IHC analysis of NECTIN2, BTLA and TNFRSF14 between imatinib resistant (upper) and sensitive (bottom) patients. f Schematic diagram of the unique tumor-immune microenvironment of imatinib-resistance in advanced GIST.

Article Snippet: Finally, two experienced pathologists independently evaluated staining results for Foxp3 (Servicebio, GB11093), BTLA (Boster, A03149), TNFRSF14 (Affinity, AB2838686) and NECTIN2 (Boster, A08081-2).

Techniques: Expressing

Journal: Cell Discovery

Article Title: Stromal architecture and fibroblast subpopulations with opposing effects on outcomes in hepatocellular carcinoma

doi: 10.1038/s41421-024-00747-z

Figure Lengend Snippet: a UMAPs showing tissue origin of fibroblasts from human perturbed-state fibroblasts atlas (left) and their expression of CAF-FAP (middle) and CAF-C7 (right) signature. b Schematic diagram of integrating scRNA-seq data of human fibroblasts collected from different cancer types and locations. c Estimated CAF-FAP (upper) and CAF-C7 (lower) abundance in pan-cancer fibroblasts, showing the expression of subcluster signature among cancer types. d Pearson correlation between CAF-FAP and CD8_PDCD1 abundance and between FAP and PDCD1 gene expression of TCGA data across 32 solid tumor types. Cell abundance was quantified by mean expression of the top 50 DEGs. Pearson correlation. e Kaplan-Meier plot showing the overall survival across 6 cancer types from TCGA based on the mean expression of TIGIT and NECTIN2 . Log-rank test. f Characterization of the inflammatory hubs in published ST data of human breast, ovarian, and endometrial cancer, showing paired histology, spatial distribution, and correlation of CAF-FAP and CD8_PDCD1, pathway activity, marker gene expression, and ligand‒receptor pairs. Pearson correlation. g Schematic diagram of the two major stromal archetypes (FR + and FR ‒ ) in HCC, displaying broad differences between FR + and FR ‒ tumors regarding fibroblast heterogeneity, multicellular interactome, and ECM properties.

Article Snippet: NECTIN2 -overexpressing ( NECTIN2 -OE) and control LX-2 cells were cultured in confocal dishes and stained with DIO (MCE) 24 h post-transfection.

Techniques: Expressing, Gene Expression, Activity Assay, Marker