pum1 Search Results


86
Thermo Fisher pum1 qt00029421
Pum1 Qt00029421, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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85
Thermo Fisher gene exp pum1 hs00206469 m1
Gene Exp Pum1 Hs00206469 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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93
Proteintech proteintech 26256 1 ap ubr7 antibody
Proteintech 26256 1 Ap Ubr7 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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93
Addgene inc lab ppl2014
Lab Ppl2014, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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87
Thermo Fisher gene exp pum1 hs00472881 m1
Gene Exp Pum1 Hs00472881 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 87 stars, based on 1 article reviews
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86
Thermo Fisher gene exp pum1 hs00982775 m1
Gene Exp Pum1 Hs00982775 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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89
OriGene pum1 rc201219 orf
FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and <t>PUM1</t> identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.
Pum1 Rc201219 Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
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92
Addgene inc ptyb3 pum1 hd
FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and <t>PUM1</t> identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.
Ptyb3 Pum1 Hd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Thermo Fisher gene exp pum1 rn01180594 m1
FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and <t>PUM1</t> identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.
Gene Exp Pum1 Rn01180594 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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94
Thermo Fisher gene exp pum1 mm01180596 m1
Quantitative PCR probes
Gene Exp Pum1 Mm01180596 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp pum1 mm01180596 m1/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
gene exp pum1 mm01180596 m1 - by Bioz Stars, 2026-03
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Image Search Results


FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and PUM1 identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and PUM1 identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques: RNA Sequencing Assay, Labeling, Over Expression

FOXM1 mRNA is regulated by the NANOS3/PUM1 post-transcriptional complex through its’ 3′UTR. ( A ) Western-blot visualization of GST pull-down of NANOS3 with and without RNase A. ( B ) Schematic representation of FOXM1 3′UTR containing the Pumilio binding element (PBE-UGUAHAUA) and 2 PBE-like motifs (containing core UGUA motif). ( C ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of FOXM1 in the downstream of Renilla ORF. Standard error is visualized as error bars. * = p -value ≤ 0.05, *** = p -value ≤ 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: FOXM1 mRNA is regulated by the NANOS3/PUM1 post-transcriptional complex through its’ 3′UTR. ( A ) Western-blot visualization of GST pull-down of NANOS3 with and without RNase A. ( B ) Schematic representation of FOXM1 3′UTR containing the Pumilio binding element (PBE-UGUAHAUA) and 2 PBE-like motifs (containing core UGUA motif). ( C ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of FOXM1 in the downstream of Renilla ORF. Standard error is visualized as error bars. * = p -value ≤ 0.05, *** = p -value ≤ 0.001.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques: Western Blot, Binding Assay, Luciferase, Over Expression

The NANOS3–PUM1–FOXM1 axis coordinates the expression of the cell cycle genes. ( A ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of CCNA2 , KIF20B , and RAD21 in the downstream of Renilla ORF. ( B ) Relative fold change of FOXM1 binding events per 1000 cells for the promoter region of the target genes measured by ChIP-qPCR. ( C ) Differential correlation analysis of NANOS3 , PUM1 , and FOXM1 in publicly available healthy testis (GTEx) and testicular cancer datasets (TCGA). Each dot represents a single GTEx or TCGA dataset. Expression values are transformed as log(expression +1). Standard error is visualized as error bars. ** = p -value ≤ 0.01, *** = p -value ≤ 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: The NANOS3–PUM1–FOXM1 axis coordinates the expression of the cell cycle genes. ( A ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of CCNA2 , KIF20B , and RAD21 in the downstream of Renilla ORF. ( B ) Relative fold change of FOXM1 binding events per 1000 cells for the promoter region of the target genes measured by ChIP-qPCR. ( C ) Differential correlation analysis of NANOS3 , PUM1 , and FOXM1 in publicly available healthy testis (GTEx) and testicular cancer datasets (TCGA). Each dot represents a single GTEx or TCGA dataset. Expression values are transformed as log(expression +1). Standard error is visualized as error bars. ** = p -value ≤ 0.01, *** = p -value ≤ 0.001.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques: Expressing, Luciferase, Over Expression, Binding Assay, Transformation Assay

Model of NANOS3-PUM1-FOXM1 axis regulating G2/M phase of the cell cycle in TCam-2 cell line. Created with BioRender.com.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: Model of NANOS3-PUM1-FOXM1 axis regulating G2/M phase of the cell cycle in TCam-2 cell line. Created with BioRender.com.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques:

Quantitative PCR probes

Journal: Journal of Lipid Research

Article Title: Evaluation of antisense oligonucleotide therapy targeting Hsd17b13 in a fibrosis mice model

doi: 10.1016/j.jlr.2024.100514

Figure Lengend Snippet: Quantitative PCR probes

Article Snippet: Pum1 , Mm01180596_m1.

Techniques: Real-time Polymerase Chain Reaction