Journal: Biomimetics

Article Title: Calcium Silicate-Based Cements for Vital Pulp Therapy: Integrated Assessment of Radiopacity, Elemental Composition, and 24-h Pulp Cell Responses

doi: 10.3390/biomimetics11040280

Figure Lengend Snippet: Viability and inflammatory response of human dental pulp stem cells following exposure to tested calcium silicate-based cements. ( A ) Cell viability assessed using MTT assay following 24-h exposure to material extracts at four serial dilutions (1:1, 1:2, 1:4, and 1:8). Cell viability is expressed as percentage relative to untreated control cells. Dashed line indicates 70% viability threshold according to ISO 10993-5 cytotoxicity standard. ( B ) Anti-inflammatory cytokine interleukin-10 (IL-10) secretion quantified by ELISA. ( C ) Pro-inflammatory cytokine interleukin-6 (IL-6) secretion quantified by ELISA. Cytokine expression (IL-6 and IL-10) was evaluated at 1:2 extract dilution, a concentration at which the majority of tested materials demonstrated acceptable cell viability (≥70% per ISO 10993-5) in the MTT assay. All data represent mean ± standard deviation from three independent experiments.

Article Snippet: Human dental pulp stem cells (hDPSCs; CELPROGEN, 36086-01, Torrance, CA, USA) were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin-streptomycin, and L-glutamine.

Techniques: MTT Assay, Control, Enzyme-linked Immunosorbent Assay, Expressing, Concentration Assay, Standard Deviation

Journal: FEBS Open Bio

Article Title: Endothelial cell‐derived extracellular vesicles induce pro‐angiogenic responses in mesenchymal stem cells

doi: 10.1002/2211-5463.13650

Figure Lengend Snippet: Effects of different dose of HU‐sEV treatment on (A) fatty acid profile and (B) gene expression levels of angiogenesis markers in MSCs as non‐differentiated negative control group and HUVEC as angiogenic positive control group * P < 0.05. (HUVEC, human embryonic vascular endothelial cells as positive vascular control; MSC, mesenchymal stem cells as untreated control). The data were statistically analyzed using one‐way ANOVA with Tukey post hoc test. The data were presented as mean values ± SEM. n = 3.

Article Snippet: HUVEC (ATCC CRL‐1730) and mesenchymal stem cell (MSC; #PT‐5025, Poietics™ Human Dental Pulp Stem Cells) cells were used for this study.

Techniques: Expressing, Negative Control, Positive Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Chitin scaffolds derived from the marine demosponge Aplysina fistularis stimulate the differentiation of dental pulp stem cells

doi: 10.3389/fbioe.2023.1254506

Figure Lengend Snippet: Ultrastructure of DPSCs cultured on the scaffold, with or without HAp, after 5 or 10 days. (A,B) Most of the DPSCs (PURE) are small, round cells, without extensive RER (arrows), although it is wider than that in DPSC cultured without the scaffold (see ). A small amount of electron-dense multivesicular bodies (arrowhead) can be found. (C) DPSCs cultured with HAp tend to internalize this mineral (arrowhead). Some of them tend to (putatively) initiate the mineralization processes (arrows). (D) Some DPSCs extend long protrusions with prominent fibers (arrows). (E,F) DPSCs surrounding the scaffold fragment are connected to each other by tight junctions (arrowheads). Cells also tend to be spindle-shaped with long protrusions. (G,H) Cells surrounded tightly by the scaffold modified with HAp. Cells tend to be spindle-shaped, with cytoplasm similar to the cells described previously.

Article Snippet: DPSCs were cultured in the DPSC Dental Pulp Stem Cell BulletKitTM Medium (Lonza), according to the manufacturer’s instructions.

Techniques: Cell Culture, Modification