ptppuc3 Search Results


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Addgene inc ptppuc3
( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid <t>(pTpPuc3)</t> compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.
Ptppuc3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.

Journal: Nature Communications

Article Title: Designer diatom episomes delivered by bacterial conjugation

doi: 10.1038/ncomms7925

Figure Lengend Snippet: ( a ) Conjugation from E. coli to T. pseudonana results in increased conjugation efficiency when the yeast CEN6-ARSH4-HIS3 region is included on the plasmid (pTpPuc3) compared with control plasmid lacking this region (pTpPuc4). Error bars denote one s.d. of the mean from at least three biological replicates. ( b , c ) Images of T. pseudonana wild type ( b ) and exconjugants expressing YFP translationally fused to PEPCK (Protein ID_5186) encoded on a p0521s-derived episome ( c ). Scale bar, 2.5 μm.

Article Snippet: E. coli strains containing plasmids p0521s (GenBank accession KP745602), pPtPuc3 (GenBank accession KP745601) and pTpPuc3 (GenBank accession KP745603) described in this manuscript have been deposited at Addgene.com and are available for request.

Techniques: Conjugation Assay, Plasmid Preparation, Control, Expressing, Derivative Assay