Journal: Animals : an Open Access Journal from MDPI

Article Title: FABP3 Mediates Lipid Droplet Accumulation and Adhesive Capacity in Bovine Endometrial Epithelial Cells via PGE 2 /PTGER4/PPAR Axis

doi: 10.3390/ani15233417

Figure Lengend Snippet: PGE 2 binds to PTGER4 to regulate FABP3 expression in bEECs: ( A ) The difference in PTGER ( PTGER1 , PTGER2 , PTGER3 and PTGER4 ) expression in bEECs after treatment with P 4 and/or PGE 2 . ( B – D ) The representative images and analysis of PTGER2 and PTGER4 expression in bEECs after treatment with P 4 and/or PGE 2 . ( E , F ) The representative images and analysis of PTGER2 expression in bEECs between shN and shPTGER2 groups after treatment with DMSO or P 4 + PGE 2 . ( G , H ) The representative images and analysis of PTGER4 expression in bEECs between shN and shPTGER4 groups after treatment with DMSO or P 4 + PGE 2 . ( I ) The analysis of FABP3 expression in bEECs after treatment with DMSO, PGE 2 or the inhibitor of PTGER4 ( GW627368 ). The data are means ± SEM of three independent experiments. Statistically significant differences are indicated by asterisks: ** p < 0.01, ns p > 0.05. Bars with different letters are significantly different ( p < 0.05).

Article Snippet: Then, PGE 2 (10 −8 –10 −6 M; 14010, Cayman, Ann Arbor, MI, USA) was added to the medium, and the cells were cultured for 24 h. For inhibitor treatment, 5 and 10 μM PTGER2 inhibitor (TG-155; MedChemExpress, Newark, NJ, USA) or 5 and 10 μM PTGER4 inhibitor ( GW627368 ; Selleck, Houston, TX, USA) were added to the medium 1 h prior to the PGE 2 treatment.

Techniques: Expressing

Journal: Mediators of Inflammation

Article Title: Diallyl Disulfide Suppresses Inflammatory and Oxidative Machineries following Carrageenan Injection-Induced Paw Edema in Mice

doi: 10.1155/2020/8508906

Figure Lengend Snippet: Effect of diallyl disulfide (DADS, 100 mg/kg) or diclofenac (20 mg/kg) on levels and mRNA expression of cyclooxygenase-2 (COX-2, Ptgs2 ) and prostaglandin E2 (PGE2) in carrageenan-induced paw edema in mice. ELISA data are represented as mean ± SD ( n = 7). qRT-PCR data are presented as mean ± SD of triplicate assays and referenced to Gapdh . a p < 0.05 indicates a significant change versus the control group; b p < 0.05 indicates a significant change versus the carrageenan-injected mice.

Article Snippet: Levels of tumor necrosis factor alpha (TNF- α ; cat. no: CSB-E04741m), interlukin-1 beta (IL-1 β ; cat. no: CSB-E04621m), interlukin-2 (IL-2; cat. no: CSB-E04627m), interlukin-10 (IL-10; cat. no: CSB-E04594m), cyclooxygenase-2 (COX-2; cat. no: CSB-E12910m), prostaglandin E2 (PGE2; cat. no: CSB-PA040059), and monocyte chemoattractant protein-1 (MCP-1; cat. no: CSB-E07430m) were assessed in a paw supernatant by ELISA using anti-mouse antibody (CUSABIO Life Sciences, Wuhan, China) based on the manufacturer's instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control, Injection

Journal: Cell communication and signaling : CCS

Article Title: PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cells.

doi: 10.1186/s12964-024-01879-1

Figure Lengend Snippet: Fig. 5 PGE2-PTGER4 activates HDACs to increase SPINK4 expression. Western blot analysis for A EP4i, B PGE2 or EP4i, or C butyrate-treated organoid protein lysates probed for HDAC4 (S246)/ HDAC5 (S259)/ HDAC7 (S155), HDAC 4, and GAPDH as a loading control. The experiment in panel A is representative of an experiment that was performed on 2 different patients. Panels B and C are representative of experiments performed on 3 different patient samples. D RT-PCR fold-change quantification of SPINK4 normalized to β-ACTIN in organoids treated with PGE2 and/or LMK-235 (n = 5 different patient samples were used in this experiment). ns = non-significant; *P < 0.05; **P < 0.01; Two-way ANOVA

Article Snippet: For detecting PTGER4 by Western blotting, rabbit anti-PTGER4 was obtained from Proteintech (cat. no. 24895–1-AP).

Techniques: Expressing, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction