Journal: Frontiers in Pharmacology

Article Title: Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validation

doi: 10.3389/fphar.2026.1747977

Figure Lengend Snippet: Chemical structure of PTE and Venn analysis. (A) The 2D chemical structure of Pterostilbene (C 16 H 16 O 3 ), CAS number: 537-42-8. (B) Venn diagram representing the overlapping of PTE targets (blue) and LIRI targets (yellow). PTE: pterostilbene, 2D: two-dimensional, CAS: chemical abstracts service.

Article Snippet: Pterostilbene (PTE, HY-N0828), LY294002 (LY, HY-10108), and Anisomycin (Ani, HY-18982) were all purchased from MedChemExpress (MCE, United States), and dissolved in dimethyl sulfoxide (DMSO, D8371, Solarbio, China) before dilution to the desired concentrations for subsequent experiments.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validation

doi: 10.3389/fphar.2026.1747977

Figure Lengend Snippet: PTE mitigates IR-induced lung injury in rats. (A) Representative images showing gross morphology, HE staining, MPO immunohistochemistry (IHC), and TUNEL staining of rat lungs following 1 h of left hilar clamping and 2 h of reperfusion. PTE was administered as a single intraperitoneal injection at the onset of reperfusion (30 mg/kg for the low-dose group, PTE-L; 60 mg/kg for the high-dose group, PTE-H). The Sham group underwent no hilar occlusion, whereas the IR group received an equivalent volume of saline. (B) Pulmonary edema after reperfusion was assessed by the lung W/D ratio. (C–E) Histopathological damage was quantitatively evaluated using lung injury score, the number of MPO-positive cells, and the number of TUNEL-positive cells. (F–I) Concentrations of TNF-α, IL-1β, IL-6, and IL-10 in lung tissue homogenates were measured by ELISA. (J–M) Relative mRNA expression levels of EGFR, MAPK8, PIK3CB and SRC were determined by qRT-PCR. HE staining: scale bar = 100 μm; MPO staining: scale bar = 50 μm; TUNEL staining: scale bar = 100 μm. Data are presented as the mean ± SD (n = 6). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs. Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001, vs. IR group; n.s = non-significant. PTE: pterostilbene, IR: ischemia-reperfusion, W/D: wet to dry, HE: hematoxylin and eosin, MPO: myeloperoxidase, TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling, ELISA: enzyme-linked immunosorbent assay, qRT-PCR: quantitative real-time polymerase chain reaction.

Article Snippet: Pterostilbene (PTE, HY-N0828), LY294002 (LY, HY-10108), and Anisomycin (Ani, HY-18982) were all purchased from MedChemExpress (MCE, United States), and dissolved in dimethyl sulfoxide (DMSO, D8371, Solarbio, China) before dilution to the desired concentrations for subsequent experiments.

Techniques: Staining, Immunohistochemistry, TUNEL Assay, Injection, Saline, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

doi: 10.1155/2023/8258354

Figure Lengend Snippet: Pterostilbene mitigates diquat-induced oxidative stress in plasma and jejunum: (a) plasma SOD activity; (b) plasma GPX activity; (c) plasma GSH level; (d) plasma H 2 O 2 level; (e) jejunal SOD activity; (f) jejunal GPX activity; (g) jejunal GSH level; (h) jejunal H 2 O 2 level; (i) jejunal GR activity; (j) jejunal TRXR activity. Data are shown as mean ± standard error, n = 6/group; ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Broilers in the CON-SS and CON-DQ groups accepted a basal diet while those in the PTS-SS and PTS-DQ groups were fed a basal diet supplemented with 400 mg/kg pterostilbene (purity ≥ 99%; #537-42-8; BOC Sciences, Shirley, NY, USA).

Techniques: Clinical Proteomics, Activity Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

doi: 10.1155/2023/8258354

Figure Lengend Snippet: Effects of pterostilbene on intestinal permeability, jejunal apoptosis index, jejunal morphology and the expression of jejunal tight junction proteins in the diquat-challenged broilers. (a) Plasma DAO activity. (b) Plasma D-lactate level. (c) TUNEL analysis was used to identify the apoptotic cells in the jejunum. (d) The quantification of jejunal TUNEL-positive cells. (e) Jejunal villus height. (f) Jejunal crypt depth. (g) The ratio of villus height to crypt depth in the jejunum. (h–j) Western blot analysis was conducted to determine the protein expression of OCLN and ZO-1 in the jejunum. (k, l) qRT-PCR analysis was performed to detect the mRNA expression of OCLN and ZO-1 in the jejunum. Data are shown as mean ± standard error, n = 6/group; ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Broilers in the CON-SS and CON-DQ groups accepted a basal diet while those in the PTS-SS and PTS-DQ groups were fed a basal diet supplemented with 400 mg/kg pterostilbene (purity ≥ 99%; #537-42-8; BOC Sciences, Shirley, NY, USA).

Techniques: Permeability, Expressing, Clinical Proteomics, Activity Assay, TUNEL Assay, Western Blot, Quantitative RT-PCR

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

doi: 10.1155/2023/8258354

Figure Lengend Snippet: Effects of pterostilbene on mitochondrial redox status and function in the jejunum of diquat-challenged broilers. (a) Mitochondrial ROS in the jejunum was measured by a fluorescence probe DHE. (b) Jejunal SOD2 activity. (c–f) The jejunal activities of mitochondrial complexes I, III, and IV and ATP synthase. (g) Jejunal ATP level. (h) Jejunal mtDNA content. (i) qRT-PCR analysis was conducted to detect the expression of genes related to mitochondrial biogenesis in the jejunum. Data are shown as mean ± standard error, n = 6/group; ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Broilers in the CON-SS and CON-DQ groups accepted a basal diet while those in the PTS-SS and PTS-DQ groups were fed a basal diet supplemented with 400 mg/kg pterostilbene (purity ≥ 99%; #537-42-8; BOC Sciences, Shirley, NY, USA).

Techniques: Fluorescence, Activity Assay, Quantitative RT-PCR, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

doi: 10.1155/2023/8258354

Figure Lengend Snippet: Pterostilbene suppresses jejunal ferroptosis of diquat-challenged broilers. (a) Jejunal iron content. (b) Jejunal MDA level. (c) Jejunal 4-HNE level. (d–h) Western blot analysis was conducted to determine the protein levels of GPX4, SLC7A11, FTH1, and ACSL4 in the jejunum. (i) qRT-PCR analysis was conducted to detect the expression of genes related to ferroptosis in the jejunum. Data are shown as mean ± standard error, n = 6/group; ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Broilers in the CON-SS and CON-DQ groups accepted a basal diet while those in the PTS-SS and PTS-DQ groups were fed a basal diet supplemented with 400 mg/kg pterostilbene (purity ≥ 99%; #537-42-8; BOC Sciences, Shirley, NY, USA).

Techniques: Western Blot, Quantitative RT-PCR, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Pterostilbene Confers Protection against Diquat-Induced Intestinal Damage with Potential Regulation of Redox Status and Ferroptosis in Broiler Chickens

doi: 10.1155/2023/8258354

Figure Lengend Snippet: Pterostilbene activates NRF2 signals in the jejunum of diquat-challenged broilers. (a–d) Western blot analysis was conducted to determine the protein levels of nuclear NRF2, HO1, and SOD2 in the jejunum. (e) qRT-PCR analysis was conducted to detect the mRNA expression of NRF2 targets in the jejunum. Data are shown as mean ± standard error, n = 6/group; ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Broilers in the CON-SS and CON-DQ groups accepted a basal diet while those in the PTS-SS and PTS-DQ groups were fed a basal diet supplemented with 400 mg/kg pterostilbene (purity ≥ 99%; #537-42-8; BOC Sciences, Shirley, NY, USA).

Techniques: Western Blot, Quantitative RT-PCR, Expressing

Journal: International Journal of Oncology

Article Title: Upregulation of PD-L1 expression by resveratrol and piceatannol in breast and colorectal cancer cells occurs via HDAC3/p300-mediated NF-κB signaling

doi: 10.3892/ijo.2018.4512

Figure Lengend Snippet: Effects of dietary polyphenols on programmed cell death ligand 1 (PD-L1) expression in human breast and colon cancer cells. (A) Chemical structure of each of the polyphenols tested. (B) The Cal51 human breast cancer cell line and HCT116 colon cancer cell line, were cultured in vitro and treated with increasing concentrations of 5 polyphenols for 48 h, respectively, namely resveratrol (Res), piceatannol (Pic), pterostilbene (PTS), trimethylstilbene (TriMRes) and myricetin. Following treatment, the cells were harvested and stained for the surface expression of PD-L1 by flow cytometry. The geometric mean of mean fluorescent intensity (MFI) of phytoerythrin (PE) area was used as the readout of PD-L1. The levels of PD-L1 were converted to a bar graph to represent the respective changes in PD-L1 expression following treatment. The parental condition (also referred to as DMSO-treated, or control cells). Statistical difference reflects the comparison of treated samples to the parental condition. The data shown were from n=3 independent experiments. * P<0.05.

Article Snippet: Pterostilbene and myricetin were from LKT Laboratories (St. Paul, MN, USA) and trimethoxy-resveratrol (trans-3,5,4′-trimethoxystilbene) was from Cayman Chemical Co. (Ann Arbor, MI, USA).

Techniques: Expressing, Cell Culture, In Vitro, Staining, Flow Cytometry, Control, Comparison

Journal: PLoS ONE

Article Title: Estrogen Receptor-α36 Is Involved in Pterostilbene-Induced Apoptosis and Anti-Proliferation in In Vitro and In Vivo Breast Cancer

doi: 10.1371/journal.pone.0104459

Figure Lengend Snippet: a . ER-α36 mRNA levels were measured in Mb231, Mb231/Si36, MCF-7, and MCF-7/ER36 cells using RT-PCR. b . The relative mRNA expressions are presented as the ratio of the OD of the corresponding mRNA bands compared to the OD of the β-actin bands. c . Western blots of ER-α36 and ER-α66 proteins in Mb231, Mb231/Si36, MCF-7, and MCF-7/ER36 cells using β-actin levels as the loading control. d . MTT assays measuring the cell proliferation of the two paired cells after control and pterostilbene treatments for 72 h. The relative cell numbers were normalized to those of their respective pterostilbene untreated controls (set as 100%). (Bars, SE. * p <0.05, ** p <0.01).

Article Snippet: Pterostilbene was obtained from Merck KGaA (Darmstadt, Germany).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: PLoS ONE

Article Title: Estrogen Receptor-α36 Is Involved in Pterostilbene-Induced Apoptosis and Anti-Proliferation in In Vitro and In Vivo Breast Cancer

doi: 10.1371/journal.pone.0104459

Figure Lengend Snippet: a . Apoptosis induction in Mb231, Mb231/Si36, MCF-7, and MCF-7/ER36 cells treated with 30 µM pterostilbene for 72 h was analyzed through flow cytometry (X-Axis: Annexin V; Y-Axis: Propidium iodide, PI). The apoptosis rates are presented as both early and late apoptotic cells compared to total cells. b . Statistical analysis of FACS in ( a ). c . Caspase-3 expression in the two paired cells after control and 30 µM pterostilbene treatment for 72 h. (Means ± SE, ** p <0.01).

Article Snippet: Pterostilbene was obtained from Merck KGaA (Darmstadt, Germany).

Techniques: Flow Cytometry, Expressing, Control

Journal: PLoS ONE

Article Title: Estrogen Receptor-α36 Is Involved in Pterostilbene-Induced Apoptosis and Anti-Proliferation in In Vitro and In Vivo Breast Cancer

doi: 10.1371/journal.pone.0104459

Figure Lengend Snippet: a . Mb231, Mb231/Si36, MCF-7, and MCF-7/ER36 cells were treated with the indicated concentrations of pterostilbene for 72 h. b Mb231, Mb231/Si36, MCF-7, and MCF-7/ER36 cells were treated with 30 µM pterostilbene for the indicated hours. Changes in p-ERK1/2 and p-Akt protein levels were analyzed through western blotting. The total ERK1/2 and Akt proteins were used as loading controls.

Article Snippet: Pterostilbene was obtained from Merck KGaA (Darmstadt, Germany).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Estrogen Receptor-α36 Is Involved in Pterostilbene-Induced Apoptosis and Anti-Proliferation in In Vitro and In Vivo Breast Cancer

doi: 10.1371/journal.pone.0104459

Figure Lengend Snippet: Mb231/Si36 and Mb231 cells were injected into the left and right breast pad, respectively of 5 to 6 weeks old nude mice. When the tumors reached about 50 mm 3 size, the mice were randomly assigned into two groups (n = 3): vehicle control (Veh) and pterostilbene treatment (+Pter). a . Tumor volumes were measured once every two days. Red arrows indicate the timed of pterostilbene administration. b . Tumor weights were calculated and are shown as a plot with the median and whiskers from minimum to maximum. c . The representative images of tumors in each group are shown. d . Paraffin-embedded tissue sections of the above tumors were subjected to H&E staining (X 40, left panels) and immunohistochemical (IHC) staining using the antibody against ER-α36 (X 100, right panels). (** p <0.01, NS: no significance).

Article Snippet: Pterostilbene was obtained from Merck KGaA (Darmstadt, Germany).

Techniques: Injection, Control, Staining, Immunohistochemical staining, Immunohistochemistry

Journal: Frontiers in Pharmacology

Article Title: Simulated Microgravity Altered the Metabolism of Loureirin B and the Expression of Major Cytochrome P450 in Liver of Rats

doi: 10.3389/fphar.2018.01130

Figure Lengend Snippet: Chemical structures of loureirin B (A) and pterostilbene (B) .

Article Snippet: Pterostilbene (used as the IS, Figure ) was purchased from Great Forest Biomedical Ltd. (Hangzhou, China).

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Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Chemical structure of resveratrol (a) and pterostilbene (b)

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

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Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Experimental design

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

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Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Summary of pharmacokinetic parameters of resveratrol and pterostilbene following a single intravenous dose

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

Techniques:

Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Plasma concentration–time curves for resveratrol and pterostilbene. Animals were orally dosed daily for 14 days, and pharmacokinetic profiles were obtained after the last dose. Symbols represent mean ± SD for n = 3

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

Techniques: Clinical Proteomics, Concentration Assay

Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Summary of pharmacokinetic parameters of resveratrol and pterostilbene following daily oral dosing for 1 and 14 days

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

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Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Summary of pharmacokinetic parameters of resveratrol glucuronide and pterostilbene glucuronide following a daily oral dosing for 1 and 14 days

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

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Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Summary of pharmacokinetic parameters of resveratrol sulfate and pterostilbene sulfate following a daily oral dosing for 1 and 14 days

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

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Journal:

Article Title: Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats

doi: 10.1007/s00280-010-1525-4

Figure Lengend Snippet: Relative AUC 0–inf values after oral administration for resveratrol, pterostilbene, and their glucuronide and sulfate metabolites

Article Snippet: Instrument calibrators and quality control (QC) samples were prepared by adding 10 μL of a stock resveratrol or pterostilbene solution (in a methanol/water mixture [v/v 50:50] to 100 μL of rat plasma (Bioreclamation Inc., Westbury, NY).

Techniques: