Journal: bioRxiv

Article Title: [ 123 I]Italia: A PARP-Directed Auger Electron-Emitting Agent for Targeted Radionuclide Therapy of Cancer

doi: 10.64898/2026.03.13.711622

Figure Lengend Snippet: A) Uptake of [ 123 I]Italia_A in PSN-1 cells at different time points. B) Efflux of [ 123 I]Italia_A in PSN1 cells. C) Comparison of u ptake and blocking of [ 123 I]Italia_A, B and racemic mix. in PSN-1, cas1, KO cells after 1 hour exposure. D) Comparison of b locking of [ 123 I]Italia_A, B and racemic mix. uptake in PSN-1 cells by panel of PARP inhibitors.

Article Snippet: Human pancreatic adenocarcinoma cells (PSN-1), and human prostate cancer cells (PC-3) were purchased from ATCC; the murine breast cancer cell line 4T1 was a kind gift from prof. Nicola Sibson, Department of Oncology, University of Oxford.

Techniques: Comparison, Blocking Assay

Journal: bioRxiv

Article Title: [ 123 I]Italia: A PARP-Directed Auger Electron-Emitting Agent for Targeted Radionuclide Therapy of Cancer

doi: 10.64898/2026.03.13.711622

Figure Lengend Snippet: A) IC 50 in PSN-1 cells (IC 50 : 2.85 nM, 95%CI: 1.98 nM-4.08 nM, R squared: 0.984 (of [ 123 I]Italia), IC 50 : 1.36 nM, 95%CI: 0.5 nM −3.67 nM, R squared: 0.899 of [ 123 I]Italia_B), IC50: 2.96 nM, 95%CI: 1.1 nM-7.9 nM, R 2 : 0.970 (of racemic mix.). B ) Cell fractionation of [ 123 I]Italia_A in PSN-1 cells: western blot. C) Cell fractionation of [ 123 I]Italia_A in different cell lines. In membrane bound fraction. D) Cytoplasm soluble fraction. E) Chromatin soluble fraction. F) Nuclear soluble fraction.

Article Snippet: Human pancreatic adenocarcinoma cells (PSN-1), and human prostate cancer cells (PC-3) were purchased from ATCC; the murine breast cancer cell line 4T1 was a kind gift from prof. Nicola Sibson, Department of Oncology, University of Oxford.

Techniques: Cell Fractionation, Western Blot, Membrane

Journal: bioRxiv

Article Title: [ 123 I]Italia: A PARP-Directed Auger Electron-Emitting Agent for Targeted Radionuclide Therapy of Cancer

doi: 10.64898/2026.03.13.711622

Figure Lengend Snippet: A) Clonogenic survival of PSN-1 and PARP1 knock out cells (PSN-1 PARP1−/− ) after exposure of cells for 1h to [ 123 I]Italia_rac, and the separated enantiomers of [ 123 I]Italia_A or _B (0-500 kBq in 2 mL of growth medium). B) Clonogenic survival of various cell lines after exposure of cells for 1h to [ 123 I]Italia_A (0-500 kBq in 2mL of growth medium).

Article Snippet: Human pancreatic adenocarcinoma cells (PSN-1), and human prostate cancer cells (PC-3) were purchased from ATCC; the murine breast cancer cell line 4T1 was a kind gift from prof. Nicola Sibson, Department of Oncology, University of Oxford.

Techniques: Knock-Out

Journal: International Journal of Environmental Research and Public Health

Article Title: Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages

doi: 10.3390/ijerph17134856

Figure Lengend Snippet: Phyllomedusa azurea (renamed for Pithecopus azureus (Cope, 1862)) (Anura: Hylidae) and the phylloseptin-1 (PSN-1) peptide. ( a ) Adult specimen of P. azurea , whose skin secretion was the first source where PSN-1 was identified (Photo: Eder A. Barbosa). ( b ) Tridimensional representation of PSN-1 predicted by PEP-FOLD 3.5/De novo peptide structure prediction .

Article Snippet: Amidated PSN-1 (FLSLIPHAINAVSAIAKHN-NH 2 ; ) was synthesized on a Prelude Peptide Synthesizer (Protein Technologies Inc. Tucson, AZ, USA).

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages

doi: 10.3390/ijerph17134856

Figure Lengend Snippet: Effect of phylloseptin-1 (PSN-1) on axenic amastigotes of Leishmania amazonensis inside infected peritoneal macrophages. Cells were treated with different concentrations (0, 1, 8 or 32 μM) of PSN-1 for 2 h. ( a ) Percentage of infected macrophages, ( b ) number of amastigotes ingested by each macrophage, ( c ) index of infection and ( d ) number of non-infected macrophages (%) versus PSN-1 concentration. Data are presented as mean ± SEM, except for ( d ) where error bars stand for SD ( n = 8 repeated experiments). Different letters (i.e., groups that do not share any letter) indicate significant differences (one-way ANOVA followed by Tukey’s multiple comparisons test; p < 0.05).

Article Snippet: Amidated PSN-1 (FLSLIPHAINAVSAIAKHN-NH 2 ; ) was synthesized on a Prelude Peptide Synthesizer (Protein Technologies Inc. Tucson, AZ, USA).

Techniques: Infection, Concentration Assay

Journal: International Journal of Environmental Research and Public Health

Article Title: Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages

doi: 10.3390/ijerph17134856

Figure Lengend Snippet: Photomicrography of amastigotes of Leishmania amazonensis inside infected-peritoneal macrophages non-treated ( a ) or treated with 1 ( b ), 8 ( c ) or 32 ( d ) µM phylloseptin-1 (PSN-1). Non-normal morphology of amastigotes in ( b ), ( c ) and ( d ); normal morphology of amastigotes in the non-treated cells ( a ). Cells were stained with Giemsa. Magnification = 1000 ×, Zeiss Axio Lab.A1 microscope. Scale bar = 4 µm.

Article Snippet: Amidated PSN-1 (FLSLIPHAINAVSAIAKHN-NH 2 ; ) was synthesized on a Prelude Peptide Synthesizer (Protein Technologies Inc. Tucson, AZ, USA).

Techniques: Infection, Staining, Microscopy

Journal: International Journal of Environmental Research and Public Health

Article Title: Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages

doi: 10.3390/ijerph17134856

Figure Lengend Snippet: Nitric oxide production as estimated by nitrite measurement (top) and hydrogen peroxide levels (bottom) in peritoneal macrophages infected with axenic amastigotes of Leishmania amazonensis treated with different concentrations (0, 1, 8 or 32 μM) of phylloseptin-1 (PSN-1). Gray columns, positive control; green columns, uninfected macrophages; orange columns, infected macrophages. Data are presented as mean ± SEM ( n = 7–8 repeated experiments). * Significantly different from the control group (unpaired t-test ; p < 0.05). § Significantly different from the respective uninfected group (unpaired t -test; p < 0.05). Different lower case (uninfected) or upper case (infected) letters (i.e., groups that do not share any letter) indicate significant differences (one-way ANOVA followed by Tukey’s multiple comparisons test; p < 0.05).

Article Snippet: Amidated PSN-1 (FLSLIPHAINAVSAIAKHN-NH 2 ; ) was synthesized on a Prelude Peptide Synthesizer (Protein Technologies Inc. Tucson, AZ, USA).

Techniques: Infection, Positive Control

Journal: International Journal of Environmental Research and Public Health

Article Title: Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages

doi: 10.3390/ijerph17134856

Figure Lengend Snippet: Transforming growth factor beta (TGF-β) levels (pg/mL) in the supernatant of peritoneal macrophages infected with axenic amastigotes of Leishmania amazonensis treated with different concentrations (0, 1, 8 or 32 μM) of phylloseptin-1 (PSN-1). Green columns, uninfected macrophages; orange columns, infected macrophages. Data are presented as mean ± SEM ( n = 7–8 repeated experiments). No significant differences were detected.

Article Snippet: Amidated PSN-1 (FLSLIPHAINAVSAIAKHN-NH 2 ; ) was synthesized on a Prelude Peptide Synthesizer (Protein Technologies Inc. Tucson, AZ, USA).

Techniques: Infection

Journal: International Journal of Environmental Research and Public Health

Article Title: Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages

doi: 10.3390/ijerph17134856

Figure Lengend Snippet: Tumor necrosis factor alpha (TNF-α; top) and interleukin-12 (IL-12; bottom) levels (pg/mL) in the supernatant of peritoneal macrophages infected with axenic amastigotes of Leishmania amazonensis treated with different concentrations (0, 1, 8 or 32 μM) of phylloseptin-1 (PSN-1). Green columns, uninfected macrophages; orange columns, infected macrophages. Data are presented as mean ± SEM ( n = 6–8 repeated experiments) for TNF-α and as box plot for IL-12 ( n = 7–8 repeated experiments). § Significantly different from the respective uninfected group (unpaired t -test; p < 0.05). Different lower case (uninfected) or upper case (infected) letters (i.e., groups that do not share any letter) indicate significant differences (one-way ANOVA followed by Tukey’s multiple comparisons test; p < 0.05).

Article Snippet: Amidated PSN-1 (FLSLIPHAINAVSAIAKHN-NH 2 ; ) was synthesized on a Prelude Peptide Synthesizer (Protein Technologies Inc. Tucson, AZ, USA).

Techniques: Infection