Journal: PeerJ

Article Title: A journey through the Corynebacterium pseudotuberculosis proteome promotes insights into its functional genome

doi: 10.7717/peerj.12456

Figure Lengend Snippet: List of the studies that contributed to characterization of the C. pseudotuberculosis proteome.

Article Snippet: SDS-PAGE and immunoblot , ATCC 19410_ ovis , ATCC 809_ ovis , ATCC 43927_ ovis , ATCC 43926_ ovis , ATCC 43924_ equi , ATCC 43925_ equi , ATCC PVAMU101_ equi , Extracellular proteins and Whole cells extract , Description of protein band patterns produced by various C. pseudotuberculosis strains. In addition to the recognition of protein by antibodies of naturally infected goats. , .

Techniques: Western Blot, Produced, Infection, Labeling, Membrane

Journal:

Article Title: Phenotypic and Genotypic Characterization of Non-Starter Lactic Acid Bacteria in Mature Cheddar Cheese

doi:

Figure Lengend Snippet: (a) RAPD profiles of type and collection strains of L. paracasei subsp. paracasei (lanes 2 to 9, LMG 14766, LMG 11473, LMG 13087T, LMG 9193, LMG 11965, LMG 13722, LMG 13717, and LMG 13724a, respectively, and lanes 11 to 14, LMG 13724b, LMG 13732, LMG 12586, and LMG 9192T, respectively), L. paracasei subsp. tolerans (lanes 15 and 16, LMG 9191aT and LMG 9191bT, respectively), and L. casei (lane 17, LMG 6904T). Lanes 1, 10, and 18 contain 100-bp molecular size markers. (b) RAPD profiles of type and collection strains of L. rhamnosus (lanes 2 to 7, LMG 10769, LMG 10775, LMG 6400Ta, LMG 6400Tb, LMG 8153, and LMG 12166, respectively), L. plantarum (lanes 9 to 16, LMG 11475, LMG 14769a, LMG 14769b, LMG 6907T, LMG 1284, LMG 14760, LMG 9205, and LMG 11460, respectively), and L. pentosus (lane 17, LMG 10755T). Lanes 1, 8, and 18 contain 100-bp molecular size markers. (c) RAPD profiles of type and collection strains of L. curvatus (lanes 2 to 6, LMG 12009, LMG 12007, LMG 9198T, LMG 12008, and LMG 11479, respectively). Lanes 1 and 7 contain 100-bp molecular size markers.

Article Snippet: The type strains of L. paracasei subsp. paracasei (LMG 13087 T , cluster 30) and L. casei subsp. casei (LMG 6904 T [ATCC 393, reclassified as L. zeae ], cluster 69) and the proposed neotype strain of L. casei subsp. casei ATCC 334 (LMG 12586, cluster 57), in place of ATCC 393 ( 12 ), were all well separated (Fig. ).

Techniques:

Journal: PLoS ONE

Article Title: Functional and Structural Analysis of a Highly-Expressed Yersinia pestis Small RNA following Infection of Cultured Macrophages

doi: 10.1371/journal.pone.0168915

Figure Lengend Snippet: (A) Total RNA was isolated from wild-type (WT) and knock-down (KD) strains of Ysr170 in Y . pestis and Y . pseudotuberculosis , and Ysr170 expression was analyzed by northern blot. (B) Growth curves of Y . pestis (black lines) and Y . pseudotuberculosis (red lines) wild-type (solid) and Ysr170 KD (red) strains were examined in response to different stress conditions up to 8 hrs at 2 hr intervals. Stress conditions include temperature (37°C), mild acidity (pH 5.5), host serum (10% FBS), iron starvation (100μM 2,2’-dipyridyl), and oxidative stress (1mM H 2 O 2 ). A representative experiment from three independent experiments is shown.

Article Snippet: We used the following bacterial strains: (1) pathogenic Y . pestis CO92 (pCD1+), (2) attenuated Y . pestis CO92 (pCD1-), and (3) pathogenic Y . pseudotuberculosis (ATCC 4284).

Techniques: Isolation, Knockdown, Expressing, Northern Blot

Journal: PLoS ONE

Article Title: Functional and Structural Analysis of a Highly-Expressed Yersinia pestis Small RNA following Infection of Cultured Macrophages

doi: 10.1371/journal.pone.0168915

Figure Lengend Snippet: (A) THP-1 cells were infected with Yersinia wild-type or KD Ysr170 strains at MOI 5 for 30 min to allow for pathogen invasion. Serial dilutions of THP-1 lysates (1:100) were spread on BHI agar plates, and colonies were counted after 48 hrs. The average mean and standard deviation from three representative experiments are shown. The ‘*’ denotes statistical significance (p<0.05) for number of colonies in the Ysr170 KD strains compared to wild-type (WT). (B) THP-1 cells were infected at MOI 5 with Y . pestis and Y . pseudotuberculosis wild-type or KD Ysr170 strains. After 24 hrs, cell supernatants were collected and analyzed by ELISA. The average mean and standard deviation from three representative experiments are shown. The ‘*’ denotes statistical significance (p<0.05) for TNF-α release in the Ysr170 KD strains compared to wild-type (WT). (C) THP-1 cells were infected at MOI 5 with Y . pestis pCD1+ and pCD1- strains that expressed wild-type or KD Ysr170. After 24 hrs, cell supernatants were collected and analyzed by ELISA. The average mean and standard deviation from three representative experiments are shown. The ‘*’ denotes statistical significance (p<0.05) for TNF-α release in the Ysr170 KD Y . pestis pCD1+ strains compared to wild-type (WT). (D) THP-1 cells were infected at MOI 4 with Y . pestis pCD1+ and pCD1- strains that expressed wild-type or KD Ysr170. After 24 hrs, total RNA was extracted and RT-PCR analysis was performed using specific probes to IL-8 and EGR1. The relative RNA levels are presented as fold expression in the KD Ysr170 strains compared to wild-type. For each sample, the RNA levels were normalized to 5S rRNA. The ‘*’ denotes statistical significance (p<0.05) for fold RNA change in IL-8 and EGR1 expression between pCD1+ and pCD1- strains. The average mean and standard deviation from three representative experiments are shown.

Article Snippet: We used the following bacterial strains: (1) pathogenic Y . pestis CO92 (pCD1+), (2) attenuated Y . pestis CO92 (pCD1-), and (3) pathogenic Y . pseudotuberculosis (ATCC 4284).

Techniques: Infection, Standard Deviation, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing