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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: The Conserved His-144 in the PsbP Protein Is Important for the Interaction between the PsbP N-terminus and the Cyt b 559 Subunit of Photosystem II
doi: 10.1074/jbc.M112.385286
Figure Lengend Snippet: Cross-linking of PsbP with PSII membranes using EDC and sulfo-NHS. NaCl-washed PSII membranes were incubated with 6.25 mm EDC and 5 mm sulfo-NHS in either (A) the presence or (B) the absence of WT PsbP. Cross-linked proteins corresponding to 2 μg of Chl were loaded onto each lane. The proteins were immunodetected with antisera against CP47, CP43, PsbO, D2, D1, PsbP, PsbR, and PsbE, as shown on each lane. Protein size markers are shown on the left. C, NaCl-washed PSII was treated with 6.25 mm EDC and 5 mm sulfo-NHS to activate the carboxyl groups on the PSII. After activation, the PSII was washed and incubated either in the presence (+PsbP) or in the absence (−PsbP) of PsbP. As a positive control, the sample used in A was analyzed simultaneously.
Article Snippet: Rabbit antibodies against D2, PsbR and
Techniques: Incubation, Activation Assay, Positive Control