Journal: Pediatric Blood & Cancer

Article Title: Blood safety and the choice of anti‐hemophilic factor concentrate

doi: 10.1002/pbc.20895

Figure Lengend Snippet: Clotting Factor Concentrates Available in the USA

Article Snippet: Alphanate , Grifols , US plasma , HLC , TNBP/PS80, dry heat , Albumin added, contains vWF , 5 (500 IU) 10 (others) , 24; R or RT (2 mos) , A; 10.

Techniques: Coagulation, Fractionation, Recombinant, Derivative Assay, Functional Assay, Purification, Adsorption

Journal: Journal of Medical Virology

Article Title: Synthesis of “Nereid,” a new phenol‐free detergent to replace Triton X‐100 in virus inactivation

doi: 10.1002/jmv.26708

Figure Lengend Snippet: Virus inactivation by S/D mixes containing TX‐100 versus compound II (TX‐100 reduced). A, Plasma‐derived model matrix was spiked with PRV before the addition of S/D mix consisting of PS80, TNBP, and TX‐100 or compound II. Duplicate runs were performed at 17°C at a final S/D mix concentration of 5% as specified for manufacturing. B, Recombinant model matrix was spiked with Xenotropic murine leukemia virus (X‐MuLV) before the addition of S/D mix consisting of PS80, TNBP, and TX‐100 or compound II. Duplicate runs were performed at 1°C at a final S/D mix concentration of 10% as specified for manufacturing. A, B, Samples were drawn after 1, 10, 30, and 60 min; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective RF. Asterisks indicate viral inactivation below the detection limit. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD , standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100

Article Snippet: Taking the ratio of SD components into account, different S/D mixes were prepared by combining PS80 (Merck; 817061) and TNBP (Merck; 100002) with either TX‐100 (Merck; 108643) or the potential surrogate compounds.

Techniques: Derivative Assay, Concentration Assay, Recombinant, Standard Deviation

Journal: Journal of Medical Virology

Article Title: Synthesis of “Nereid,” a new phenol‐free detergent to replace Triton X‐100 in virus inactivation

doi: 10.1002/jmv.26708

Figure Lengend Snippet: Virus inactivation by S/D mixes containing novel compound III. Matrices were spiked with the respective virus before the addition of S/D mix (PS80, TNBP, compound III). Samples were drawn 1, 10, 30, and 60 min after S/D mix addition; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective RF. A, Duplicate runs were performed at 17°C using a plasma‐derived model matrix and PRV; the final S/D mix concentrations were 5% of manufacturing. Asterisks indicate viral inactivation below detection limit. B, Duplicate runs were performed at 1°C using a recombinant model matrix and Xenotropic murine leukemia virus (X‐MuLV); the final S/D mix concentrations were 10% of manufacturing. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD , standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100

Article Snippet: Taking the ratio of SD components into account, different S/D mixes were prepared by combining PS80 (Merck; 817061) and TNBP (Merck; 100002) with either TX‐100 (Merck; 108643) or the potential surrogate compounds.

Techniques: Derivative Assay, Recombinant, Standard Deviation

Journal: Journal of Medical Virology

Article Title: Synthesis of “Nereid,” a new phenol‐free detergent to replace Triton X‐100 in virus inactivation

doi: 10.1002/jmv.26708

Figure Lengend Snippet: Virus inactivation by S/D mixes containing novel compounds IV and V. Duplicate runs were performed at 17°C using a plasma‐derived model matrix and PRV. PS80 and TNBP were combined with (A) compound IV or (B) compound V and added to the virus‐spiked matrix to reach a final concentration of 5% as specified for manufacturing. A, B, Samples were drawn 1, 10, 30, and 60 min after S/D mix addition; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective reduction factor (RF). Asterisks indicate viral inactivation below detection limit. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD , standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100

Article Snippet: Taking the ratio of SD components into account, different S/D mixes were prepared by combining PS80 (Merck; 817061) and TNBP (Merck; 100002) with either TX‐100 (Merck; 108643) or the potential surrogate compounds.

Techniques: Derivative Assay, Concentration Assay, Standard Deviation

Journal: Journal of Medical Virology

Article Title: Synthesis of “Nereid,” a new phenol‐free detergent to replace Triton X‐100 in virus inactivation

doi: 10.1002/jmv.26708

Figure Lengend Snippet: Virus inactivation by S/D mixes containing novel compounds VI and VII. Matrices were spiked with the respective virus before addition of S/D mix (PS80, TNBP, compound VI/compound VII). Samples were drawn 1, 10, 30, and 60 min after S/D mix addition; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective RF. A, B, Duplicate runs were performed at 17°C using a plasma‐derived model matrix and PRV; the final S/D mix concentrations were 5% of manufacturing. Asterisks indicate viral inactivation below the detection limit. C, D, Duplicate runs were performed at 1°C using a recombinant model matrix and Xenotropic murine leukemia virus (X‐MuLV); the final S/D mix concentrations were 10% of manufacturing. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD , standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100

Article Snippet: Taking the ratio of SD components into account, different S/D mixes were prepared by combining PS80 (Merck; 817061) and TNBP (Merck; 100002) with either TX‐100 (Merck; 108643) or the potential surrogate compounds.

Techniques: Derivative Assay, Recombinant, Standard Deviation

Journal: Journal of Medical Virology

Article Title: Synthesis of “Nereid,” a new phenol‐free detergent to replace Triton X‐100 in virus inactivation

doi: 10.1002/jmv.26708

Figure Lengend Snippet: Virus inactivation by S/D mixes containing novel compounds XIII and XIV. Duplicate runs were performed at 17°C using a plasma‐derived model matrix and PRV. PS80 and TNBP were combined with (A) compound XIII or (B) compound XIV and added to the virus‐spiked matrix to reach a final concentration of 5% as specified for manufacturing. Samples were drawn 1, 10, 30, and 60 min after S/D mix addition; viral loads of these samples were compared to a sample drawn before S/D mix addition to calculate the respective RF. Asterisks indicate viral inactivation below detection limit. SD only shown if larger than the height of symbols). PRV, pseudorabies virus; RF, reduction factor; S/D, solvent/detergent; SD , standard deviation; TNBP, tri‐n‐butyl‐phosphate; TX‐100, Triton X‐100

Article Snippet: Taking the ratio of SD components into account, different S/D mixes were prepared by combining PS80 (Merck; 817061) and TNBP (Merck; 100002) with either TX‐100 (Merck; 108643) or the potential surrogate compounds.

Techniques: Derivative Assay, Concentration Assay, Standard Deviation