Journal: PLoS ONE

Article Title: Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells

doi: 10.1371/journal.pone.0157126

Figure Lengend Snippet: (A-J) Embryonic tissues: bright field (A) and fluorescence (B) images of the Prox1-tdTomato embryo (E17.5). Distinct lymphatic networks shown in the embryonic skin (C-E). (E) Enlarged image of the boxed area in panel D. Lymphatic vessels in the embryonic liver (F,G) and mesentery membrane (H). Note that hepatocytes (I) and tail nerves (J) are also positive for tdTomato. (K-Y) Adult tissues: headshots of adult wild type and Prox1-tdTomato transgenic mice were taken under a bright light (K), bright and fluorescent light (L) and fluorescent light (M). Lymphatic vessels were easily detectable in various tissues such as the ear (N), eye (O,P), tail (Q), tongue (R), trachea (S), diaphragm muscle (T), bladder (U,V), intestine (W), mesentery (X) and lymph node (Y). Corneal limbal lymphatic (arrow) and Schlemm’s canal (arrowhead) of the eye were shown in two consecutive focal planes (O,P). Bilateral lymphatic collectors in the tail were marked with two arrows (Q).

Article Snippet: Prox1 staining was performed using anti-Prox1 antibody (R&D Systems).

Techniques: Fluorescence, Membrane, Transgenic Assay

Journal: PLoS ONE

Article Title: Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells

doi: 10.1371/journal.pone.0157126

Figure Lengend Snippet: (A-H) Axillary lymphadenectomy model: Fluorescent light enables the researchers to easily locate the axillary lymph node (A-D). From a different set of mice, the left-side axillary lymph nodes were removed, while the right-side nodes were left intact. To enhance lymphatic growth in the surgical site, 9-cis RA was treated for the first 5 days after surgery. After 4 weeks, lymphatic vessels were visualized in the left vs . right side of the armpit of the mice with asterisks indicating the original locations of the lymph nodes (E,F). Images in the boxed areas (E, F) are shown in panels G and H. (I-Q) Lymph node transplantation model: the axillary lymph node in Prox1-tdTomato mice (recipient) was replaced with the matching lymph node from Prox1-EGFP mice (donor), followed by administration of 9-cis RA for 4 weeks. Panels O, P and Q are enlarged images of the boxed areas in panels K, M and N, respectively. (R-U) Tail lymphedema model: representative images of the circumcised tails at post-operational days 1 and 15 are shown (R,T). At both days, the circumcised tail skins were prepared inside-out to visualize dermal lymphatic vessels (S,U). Asterisks mark the wounded area and black dotted lines indicate the wound border. Note the numerous newly formed lymphatics invading into the healed area (S).

Article Snippet: Prox1 staining was performed using anti-Prox1 antibody (R&D Systems).

Techniques: Transplantation Assay

Journal: PLoS ONE

Article Title: Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells

doi: 10.1371/journal.pone.0157126

Figure Lengend Snippet: (A-E) Bladder lymphangiogenesis model: Adult Prox1-tdTomato mice were i.p. injected with phosphate-buffered saline (A,C) or cyclophosphamide (B,D) at days 1 and 4. At day 9, lymphatic vessels in their bladders were visualized. Morphometric analyses revealed a significantly increase in the number of branch (marked with arrowhead), loop (arrow) and blind ends (double arrowhead) of lymphatic vessels in the CYP-treated bladder compared to the PBS-treated control (CTR) bladder (E). (F-J) Tumor implantation model: GFP-labeled tumor cells were implanted into the skin of immunodeficient athymic Prox1-tdTomato mouse, generated by crossing athymic nude mice and Prox1-tdTomato mice. Images of the GFP-labeled tumor mass (F), dermal lymphatic in the surrounding tissues (G) and their merged image (H) are shown. (I) and (J) are enlarged images of the boxed areas in panel (H) and (I), respectively. Arrows mark luminal valves.

Article Snippet: Prox1 staining was performed using anti-Prox1 antibody (R&D Systems).

Techniques: Injection, Saline, Control, Tumor Implantation, Labeling, Generated

Journal: PLoS ONE

Article Title: Efficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells

doi: 10.1371/journal.pone.0157126

Figure Lengend Snippet: (A,B) Bright field (BF) and fluorescent (UV) images of the head of Prox1-tdTomato, Prox1-tdTomato/Prox1-EGFP and Prox1-EGFP lymphatic-specific reporter mice. (C-K) Expressions of tdTomato and/or EGFP proteins were visualized in the skin of embryos (EM, C-E) and new pups (P1, F-H), or in the bladder of adult (BL, I-J) of each genotype. (L-N) Prox1 whole-mount staining of the ear of tdTomato adult mouse.

Article Snippet: Prox1 staining was performed using anti-Prox1 antibody (R&D Systems).

Techniques: Staining

Journal: Cell reports

Article Title: Early Seizure Activity Accelerates Depletion of Hippocampal Neural Stem Cells and Impairs Spatial Discrimination in an Alzheimer’s Disease Model

doi: 10.1016/j.celrep.2019.05.101

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-Prox1 , PhosphoSolutions , Cat#: 1685-Prox1; RRID: AB_2492217.

Techniques: Recombinant, Imaging, Software, Microscopy

Journal: bioRxiv

Article Title: Somatic variants activating the RAS-MAPK pathway confer susceptibility to hippocampal sclerosis in drug-resistant epilepsy

doi: 10.64898/2026.04.06.716727

Figure Lengend Snippet: Variant allele fractions (VAFs) from amplicon sequencing were compared across brain regions and cell types (A) . In four of six cases with findings, the causal somatic variant was identified in both cortex and hippocampus tissue (B) . One case with a PTPN11 A461T variant exhibited extensive neuronal loss in the CA1 region, as seen in H&E and NeuN stains (C) . Fluorescence-activated nuclei sorting was used to isolate hippocampal cell types from this tissue for amplicon sequencing (D) . The PTPN11 A461T variant was present in all cell types but highly enriched among Prox1-neurons (E) .

Article Snippet: Nuclei were labeled using the following antibodies: NeuN (Millipore, #MAB377X), PROX1 (Novus Biologicals, #NBP1-30045AF647), OLIG2 (Novus Biologicals, # NBP2-89201AF594), and PAX6 (Novus Biologicals, #NBP2-34705AF647) at 1:100 followed by incubation for 1 hour at 4°C with agitation.

Techniques: Variant Assay, Amplification, Sequencing, Fluorescence

Journal: bioRxiv

Article Title: Somatic variants activating the RAS-MAPK pathway confer susceptibility to hippocampal sclerosis in drug-resistant epilepsy

doi: 10.64898/2026.04.06.716727

Figure Lengend Snippet: Sorting scheme to enrich astrocytes, oligodendrocytes, Prox1-negative neurons and Prox1-positive neurons for amplicon sequencing.

Article Snippet: Nuclei were labeled using the following antibodies: NeuN (Millipore, #MAB377X), PROX1 (Novus Biologicals, #NBP1-30045AF647), OLIG2 (Novus Biologicals, # NBP2-89201AF594), and PAX6 (Novus Biologicals, #NBP2-34705AF647) at 1:100 followed by incubation for 1 hour at 4°C with agitation.

Techniques: Amplification, Sequencing