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Image Search Results
Journal: Molecular Pain
Article Title: Characterisation of Nav1.7 functional expression in rat dorsal root ganglia neurons by using an electrical field stimulation assay
doi: 10.1177/1744806917745179
Figure Lengend Snippet: ProTx-II blocks sodium channel currents in DRG neurons. (a) Whole cell patch-clamp recordings in DRG and hippocampal neurons were performed to investigate the effect of TTX and ProTx-II on sodium currents (elicited by a test voltage step of 25 ms duration from −80 mV holding potential to −20 mV). Representative sodium current traces from DRG and hippocampal neurons are shown before (black line) and after application of 100 nM TTX (top panel, blue trace) and 300 nM ProTx-II (bottom panel, red trace) for a total duration of 5 min. (b) Averaged data for the experiment in (a) showing normalised amplitude of sodium currents in DRG and hippocampal neurons after incubation with ProTx-II (300 nM) and TTX (100 nM); n = 3–6 cells, ***p < 0.001.
Article Snippet: TTX was purchased from Tocris (Bristol, UK) and
Techniques: Patch Clamp, Incubation