Journal: Journal of Molecular Neuroscience

Article Title: Inflammatory Response and Chemokine Expression in the White Matter Corpus Callosum and Gray Matter Cortex Region During Cuprizone-Induced Demyelination

doi: 10.1007/s12031-012-9773-x

Figure Lengend Snippet: a HE-stained sections of the midline of the corpus callosum in control and 2 days cuprizone-treated animals as well as a 2-day cortex sample. On day 2 after the start of the cuprizone diet, cells with the typical morphological characteristics of apoptosis, such as round, condensed, and fragmented nuclei, were detected in both regions ( red and blue arrows ). The apoptotic nature was further verified by anti-caspase 3 staining ( lower left ) and anti-caspase 3/CNPase double labeling ( lower right ). b Left part Anti-APC stained sections of the midline of the corpus callosum as well as cortex regions of a control animal and after 5 weeks of cuprizone exposure. Right part Results of APC-expressing cell quantification within the corpus callosum and cortex regions. c Anti-PLP-stained sections of the midline of the corpus callosum and cortex regions in control and 2 days and 1, 3, and 5 weeks cuprizone-treated animals. Note that no overt loss of PLP staining intensity can be observed until week 1 in both regions included in the study, whereas the midline of the corpus callosum and the cortex regions are significantly demyelinated at week 3. Demyelination further progresses until week 5. d Results of Plp gene expression analysis of the entire corpus callosum or cortex region. Each bar represents the averaged fold induction over untreated control mice of at least four mice per time point (±SEM). Values were normalized against a housekeeping gene ( 18s ) and expressed relative to the respective control levels. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 treatment vs. control. Scale bars , 15 μm ( upper part in a ); 10 μm ( lower left in a ); 5 μm ( lower right in a ); 50 μm ( b ); and 80 μm ( c )

Article Snippet: Anti-proteolipid protein antibody (PLP, 1:500, mouse IgG; Serotec, Germany) was used as a myelin marker; anti-adenomatous polyposis coli (APC, 1:200, mouse IgG; Calbiochem, Germany) was used to stain late-stage oligodendrocyte cell bodies, while anti-glial fibrillary acidic protein (GFAP, 1:1,000, rabbit IgG; Encore, USA) was used to visualize astrocytes.

Techniques: Staining, Control, Labeling, Expressing, Gene Expression

Journal: BioMed Research International

Article Title: PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma

doi: 10.1155/2020/4286101

Figure Lengend Snippet: PLP2 was a high-risk myeloma gene. (a) PLP2 expression of NPC ( n = 22), MGUS ( n = 44), SMM ( n = 12), and MM (TT2, n = 351; TT3, n = 208) in GSE5900 and GSE2658 datasets ( ∗∗ p < 0.01, ∗∗∗ p < 0.001). (b) A scatter plot showing the PLP2 expression in eight MM subgroups (CD1 and CD2 subgroups with spiked expression of CCND1 and CCND3; PR: proliferation; LB: low-bone disease; HY: hyperdiploid; MS: MMSET; MF: MAFB; MY: myeloid). (c) CD138 and PLP2 expressions in the bone marrow of NDMM patients. Representative case with a lack of PLP2 expression: H&E stain, CD138 immunostain, and PLP2 immunostain (upper row). Representative case with stable PLP2 expression: H&E stain, CD138 immunostain, and PLP2 immunostain (lower row).

Article Snippet: Formalin-fixed, paraffin-embedded sections were utilized for IHC with the following antibodies: CD138 (Proteintech, USA) and PLP2 (Proteintech, USA), according to the manufacturer's protocol [ ].

Techniques: Expressing, Staining

Journal: BioMed Research International

Article Title: PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma

doi: 10.1155/2020/4286101

Figure Lengend Snippet: PLP2 was linked to myeloma progression. (a–d) β 2-MG, LDH, bone marrow infiltration, and ISS stages were expressed the highest in the high PLP2 expression subgroup, while the lowest in the low PLP2 expression subgroup ( ∗ p < 0.05). (e–g) β 2-MG and CRP were expressed the highest in the PLP2 high subgroup, while ALB was expressed the lowest in the PLP2 high subgroup ( ∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: Formalin-fixed, paraffin-embedded sections were utilized for IHC with the following antibodies: CD138 (Proteintech, USA) and PLP2 (Proteintech, USA), according to the manufacturer's protocol [ ].

Techniques: Expressing

Journal: BioMed Research International

Article Title: PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma

doi: 10.1155/2020/4286101

Figure Lengend Snippet: High PLP2 expression was linked to a poor prognosis in two independent datasets. (a, b) Kaplan-Meier analyses of OS and PFS revealed that strong PLP2 staining intensity conferred inferior clinical outcomes in our cohort. (c, d) Kaplan-Meier analyses of OS and PFS revealed that high PLP2 gene expression conferred inferior clinical outcomes in GSE24080.

Article Snippet: Formalin-fixed, paraffin-embedded sections were utilized for IHC with the following antibodies: CD138 (Proteintech, USA) and PLP2 (Proteintech, USA), according to the manufacturer's protocol [ ].

Techniques: Expressing, Staining, Gene Expression

Journal: BioMed Research International

Article Title: PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma

doi: 10.1155/2020/4286101

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses for OS in 87 NDMM patients.

Article Snippet: Formalin-fixed, paraffin-embedded sections were utilized for IHC with the following antibodies: CD138 (Proteintech, USA) and PLP2 (Proteintech, USA), according to the manufacturer's protocol [ ].

Techniques:

Journal: BioMed Research International

Article Title: PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma

doi: 10.1155/2020/4286101

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses for PFS in 87 NDMM patients.

Article Snippet: Formalin-fixed, paraffin-embedded sections were utilized for IHC with the following antibodies: CD138 (Proteintech, USA) and PLP2 (Proteintech, USA), according to the manufacturer's protocol [ ].

Techniques:

Journal: BioMed Research International

Article Title: PLP2 Expression as a Prognostic and Therapeutic Indicator in High-Risk Multiple Myeloma

doi: 10.1155/2020/4286101

Figure Lengend Snippet: PLP2 was related to treatment response. The correlations between drug responses with PLP2 levels were analyzed by chi-square test. Patients with low PLP2 expression significantly responded to chemotherapies evidenced by increased ORR, ≥VGPR, ≥CR, and ≥sCR.

Article Snippet: Formalin-fixed, paraffin-embedded sections were utilized for IHC with the following antibodies: CD138 (Proteintech, USA) and PLP2 (Proteintech, USA), according to the manufacturer's protocol [ ].

Techniques: Expressing

Journal: eLife

Article Title: LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair

doi: 10.7554/eLife.30498

Figure Lengend Snippet:

Article Snippet: Primary antibodies included: rat anti-PDGFRα (BD pharmingen, 558774, 1:500), rabbit anti-CNPase (Aves Labs, 27490 R12-2096, Tigard, OR, 1:500), mouse anti-MAG (Millipore, MAB1567, 1:500), rat anti-MBP (Millipore, MAB386, 1:1000), chicken anti-PLP (Aves Labs, 27592, 1:500), mouse anti-GFAP (Sigma, G3893, 1:1000), chicken anti-GFAP (Aves Labs, GFAP, 1:500), rabbit anti-NG2 (Millipore, AB5320, 1:500), rabbit anti-LRP1β (Abcam, ab92544, 1:500), rabbit anti-PMP70 (Thermo, PA1-650, Waltham, MA, 1:1000).

Techniques: Sequencing, Quantitation Assay, Software, Western Blot

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A ) A Venn diagram illustrates the co-expressed genes identified in two datasets ( GSE100534 and GSE52604 , P < 0.01, logFC ≥ 1.5). ( B , C ) Heatmaps visualize 26 co-expressed genes from datasets GSE100534 (BTT vs. BCBMT) and GSE52604 (NNBT vs. BCBMT), organized according to the HR of DMFS derived from Kaplan–Meier analyses. ( D , E ) Kaplan–Meier survival curves depict DMFS and OS probabilities of BC patients, stratified by high and low expression levels of LRP8. ( F ) Box plots illustrate the expression levels of LRP8 across breast cancer patients and normal individuals. Normal (minimum: 0.127, Q1: 0.858, median: 1.085, Q3: 1.354, maximum: 2.207), BC (minimum: 0.126, Q1: 1.209, median: 2.267, Q3: 4.518, maximum: 11.792). P = 1 × 10 −12 . ( G ) Box plots illustrate the expression levels of LRP8 across various breast cancer subtypes and normal individuals. Normal (minimum: 0.127, Q1: 0.858, median: 1.085, Q3: 1.354, maximum: 2.207), luminal (minimum: 0.126, Q1: 1.045, median: 1.765, Q3: 3.269, maximum: 7.787), HER2 + (minimum: 0.497, Q1: 3.551, median: 5.129, Q3: 10.331, maximum: 20.231), triple negative (minimum: 0.404, Q1: 4.661, median: 7.863, Q3: 10.133, maximum: 19.513). P = 2.3 × 10 −16 (normal vs. Luminal), P = 6 × 10 −8 (normal vs . HER2 + ), P = 1.63 × 10 −12 (normal vs. triple negative). ( H ) Immunohistochemistry staining for LRP8 was performed on human TNBC tissues and paracancerous tissues. Scale bar: 100 μm. ( I ) IHC scores of ( H ) were presented. n = 10. ( J ) Western blot analysis was performed on MCF-10A, MCF-7, BT549, and MDA-MB-231 cell lines to evaluate LRP8 protein expression levels. ( K ) Quantitative analysis revealed the relative LRP8 protein level normalized to β-tubulin. n = 3. BTT breast tumor tissue, NNBT non-neoplastic breast tissue, BCBMT breast cancer brain metastasis tissue, DMFS distant metastasis-free survival, OS overall survival, HR hazard ratio. Data information: data are shown as mean ± SD. P values were analyzed with unpaired Student’s t test ( F ), Mann–Whitney test ( I ) and one-way ANOVA test ( G , K ). ns non-significant. .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Derivative Assay, Expressing, Immunohistochemistry, Staining, Western Blot, MANN-WHITNEY

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A ) MDA-MB-231 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # were subjected to western blot assay to analyze the protein level of LRP8. ( B ) Quantitative analysis showed the relative LRP8 protein level normalized to β-tubulin, n = 3. ( C ) Cell viability of MDA-MB-231 cells was evaluated by the CCK-8 assays. n = 3. P = 2.5 × 10 −7 ( sh-ctrl vs. sh-LRP8-1 # ), P = 5 × 10 −11 ( sh-ctrl vs. sh-LRP8-2 # ). ( D ) Images of the number of colonies formed from MDA-MB-231 cells in three cell lines. Scale bar: 500 μm. ( E ) Images of GFP cancer cell proliferation in zebrafish after transplantation. Scale bar: 200 μm. ( F ) Following injection of MDA-MB-231 (GFP + ) cells into zebrafish, whole-mount immunofluorescence staining for KI67 (red signal) and GFP was conducted on the embryos. Scale bar: 50 μm. ( G ) Transwell migration assay and Matrigel transwell invasion assay were performed in MDA-MB-231 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # . Scale bar: 200 μm. ( H ) Quantification of the number of colonies formed from MDA-MB-231 cells in three cell lines. n = 3. ( I ) Quantitative analysis of E showed the relative fluorescence intensity of GFP in vivo. The measurement of fluorescence of GFP at 24 hpi was used as the baseline. n = 8. ( J ) Quantitative analysis of F showed the KI67 positive cell rate in two cell lines in vivo. n = 5. P = 7.3 × 10 −5 . ( K ) Quantitative analysis of G showed the migratory abilities in three cell lines of MDA-MB-231 cells. n = 3. P = 8.2 × 10 −5 ( sh-ctrl vs. sh-LRP8-1 # ), P = 6.5 × 10 −5 ( sh-ctrl vs. sh-LRP8-2 # ). ( L ) Quantitative analysis of G showed the invasive abilities in three cell lines of MDA-MB-231 cells. n = 3. P = 8.8 × 10 −5 ( sh-ctrl vs. sh-LRP8-1 # ), P = 6 × 10 −5 ( sh-ctrl vs. sh-LRP8-2 # ). ( M ) Real-time in vivo imaging was conducted at 24 hpi, 36 hpi, 48 hpi, 60 hpi and 72 hpi to monitor the dynamic behavior of MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # . The white arrow heads indicated the disseminated cancer cells. n = 3. Scale bar: 100 μm. Data information: data are shown as mean ± SD, P values were analyzed with one-way ANOVA test ( B , H , K , L ), unpaired Student’s t test ( I , J ) and two-way ANOVA test ( C ). .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Transfection, Western Blot, CCK-8 Assay, Transplantation Assay, Injection, Immunofluorescence, Staining, Transwell Migration Assay, Transwell Invasion Assay, Fluorescence, In Vivo, In Vivo Imaging

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A ) BT549 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # were subjected for western blot assay to analyse the protein level of LRP8. ( B ) Quantitative analysis showed the relative LRP8 protein level normalized to β-tubulin, n = 3. ( C ) Cell viability of BT549 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # was evaluated using the CCK-8 assays, n = 3. ( D , E ) Images and quantification of the number of colonies formed from BT549 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # . Scale bar: 500 μm, n = 3. ( F ) MDA-MB-231 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # were immunostained with KI67 (pure signal) and nuclei were stained by DAPI (blue signal). Scale bar: 100 μm. ( G ) Quantitative analysis of KI67 positive cell rate in three cell lines of MDA-MB-231 cells in vitro, n = 7. P = 7.8 × 10 −8 ( sh-ctrl vs. sh-LRP8-1 # ), P = 1.7 × 10 −8 ( sh-ctrl vs. sh-LRP8-2 # ). ( H ) BT549 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # were immunostained with KI67 (pure signal) and nuclei were stained by DAPI (blue signal). Scale bar: 100 μm. ( I ) Quantitative analysis of KI67 positive cell rate in three cell lines of BT549 cells in vitro, n = 4. Data information: data are shown as mean ± SD, P values were analyzed with one-way ANOVA test ( B , E , G , I ) and two-way ANOVA test ( C ). .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Transfection, Western Blot, CCK-8 Assay, Staining, In Vitro

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A , B ) The investigation of wound healing capabilities in MDA-MB-231 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # were depicted. Assessment of the wound area ratio in 24 h in comparison to baseline measurements at 0 h. Scale bar: 200 μm, n = 7. ( C , D ) Investigation of wound healing capabilities in BT549 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # . Assessment of the wound area ratio in 24 h in comparison to baseline measurements at 0 h. Scale bar: 200 μm, n = 3. ( E – G ) Transwell migration assay and matrigel transwell invasion assay in BT549 cells transfected with sh-ctrl , sh-LRP8-1 # and sh-LRP8-2 # . Scale bar: 200 μm, n = 3. P = 8.3 × 10 −6 ( sh-ctrl vs. sh-LRP8-1 # ), P = 8.4 × 10 −6 ( sh-ctrl vs. sh-LRP8-2 # ). Data information: data are shown as mean ± SD, P values were analyzed with one-way ANOVA test ( B , D , F , G ). .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Transfection, Comparison, Transwell Migration Assay, Transwell Invasion Assay

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A ) Top 20 of Reactome enrichment of downregulated genes in MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # . FC >｜2｜and FDR < 0.05. ( B ) The heatmap visualization analyses demonstrating the significantly differentially expressed GEFs in MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # . ( C ) The PPI network analysis conducted by STRING revealed the interaction of CDC42 and GEFs. ( D ) The classical Rho GTPases cycle involves both an inactive GDP-bound form and an active GTP-bound form. ( E ) Quantitative real-time PCR (qRT-PCR) assays were used to measure the mRNA levels of GEFs in MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # . n = 3. P = 2 × 10 −13 ( ARHGEF38), P = 1 . 5 × 10 −9 (ARHGEF37), P = 4.5 × 10 −9 (ARHGEF16), P = 8.7 × 10 −7 (FGD4). ( F , G ) MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # were subjected to western blot assay to analyze the protein level of activated GTP-bound CDC42. n = 4. P = 1.2 × 10 −5 . ( H ) MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # were stained with TRITC-labelled phalloidin (red) and DAPI (blue) to detect the formation of filopodia. The white arrow heads indicated the filopodia of MDA-MB-231 cells. Scale bar: 10 μm. ( I , J ) Quantification analysis of the number of filopodia per cell ( n = 12, P = 4.3 × 10 −7 ) and average filopodia length ( n = 14, P = 8.8 × 10 −10 ) of MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # . FC: fold change. FDR : false discovery rate. Data information: data are shown as mean ± SD, P values were analyzed with unpaired Student’s t test ( G , I , J ) and two-way ANOVA test ( E ). ns non-significant. .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Transfection, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Staining

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A , B ) MDA-MB-231 cells were subjected for western blot assay to analyze the protein level of LRP8 after Reelin treatment. n = 3. ( C , E ) Images and quantification of the number of colonies formed from MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # , following Reelin treatment. Scale bar: 500 μm, n = 3. ( D , F ) MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # were immunostained with KI67 (pure signal) and nuclei were stained by DAPI (blue signal) after Reelin treatment. Scale bar: 100 μm, n = 3. ( G ) Wound healing capabilities were investigated in MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # after Reelin treatment. Scale bar: 200 μm. ( H ) Assessment of the wound area ratio in 24 h in comparison to baseline measurements at 0 h. n = 5. ( I , J ) Investigation and quantitative analysis of wound healing capabilities of BM0 cells and BM6 cells. Scale bar: 200 μm, n = 3. Data information: data are shown as mean ± SD, P values were analyzed with two-way ANOVA test ( E , F , H ) and unpaired Student’s t test ( B , J ). ns non-significant. .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Western Blot, Transfection, Staining, Comparison

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A ) Transwell migration assay and Matrigel transwell invasion assay were performed in MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # following Reelin treatment. Scale bar: 200 μm. ( B , C ) Quantity of migrated and invasive cells. n = 5. ( D ) MDA-MB-231 cells transfected with sh-ctrl and sh-LRP8-2 # were subjected to western blot assay to analyze the protein level of activated GTP-bound CDC42, following Reelin treatment. ( E ) Quantitative analysis revealed the relative GTP-bound CDC42 level normalized to total CDC42. n = 3. P = 4.9 × 10 -5 ( sh-ctrl ). ( F , G ) Images and quantification analysis of the migrated distance of brain metastatic cells along PceV in zebrafish models at 72 hpi after the anti-Reelin monoclonal antibody treatment. n = 9. Scale bar: 100 μm. ( H ) The schematic representation illustrates the isolation of brain metastatic cells after the transplantation of MDA-MB-231 (GFP + ) cells into zebrafish. ( I ) The mRNA expression of LRP8 in various brain metastatic cell populations. n = 3. P = 0.0091 (BM0 vs. BM4), P = 0.0027 (BM0 vs. BM5), P = 3 × 10 −5 (BM0 vs. BM6). ( J ) The expression of cell migration related genes from GO analysis. n = 86. BM0 (minimum: 0, Q1: 0.097, median: 1.377, Q3: 14.297, maximum: 871.831), BM6 (minimum: 0.161, Q1: 1.319, median: 5.963, Q3: 38.809, maximum: 2572.556). FC >｜2｜and FDR < 0.05. ( K ) The expression of cytoskeleton organization related genes from GO analysis. n = 87. BM0 (minimum: 0, Q1: 0.2095, median: 2.637, Q3: 14.2505, maximum: 282.875), BM6 (minimum: 0.327, Q1: 1.415, median: 7.716, Q3: 34.227, maximum: 1053.12). FC >｜2｜and FDR < 0.05. ( L ) Scatter plot of significant differentially expressed genes between BM0 vs. BM6. ( M ) Transwell migration assay and Matrigel transwell invasion assay were performed in BM0 cells and BM6 cells, Scale bar: 200 μm. ( N , O ) Quantification analysis of migrated and invaded BM0 or BM6 cells. n = 3. ( P ) Images of BM0 and BM6 cells migrating along PceV in zebrafish at 24 hpi. The white arrow heads indicated the disseminated cancer cells. Scale bar: 100 μm. ( Q ) Quantification analysis of migrated distance of brain metastatic cells along PceV in zebrafish. n = 9. P = 9.1 × 10 −6 . FC fold change, FDR false discovery rate. Data information: data are shown as mean ± SD, P values were analyzed with paired Student’s t test ( J , K ), unpaired Student’s t test ( N , O , Q ), two-way ANOVA test ( B , C , E ), Mann–Whitney test ( G ) and one-way ANOVA test ( I ). ns non-significant. .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Transwell Migration Assay, Transwell Invasion Assay, Transfection, Western Blot, Isolation, Transplantation Assay, Expressing, Migration, MANN-WHITNEY

Journal: EMBO Molecular Medicine

Article Title: Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration

doi: 10.1038/s44321-025-00260-0

Figure Lengend Snippet: ( A ) Three-dimensional binding pattern diagram of two candidate compounds and LRP8 protein. ( B , C ) The root mean square deviation fluctuations and potential energy fluctuations of compounds with LRP8 protein. ( D ) Representative confocal microscopy images of zebrafish xenograft models treated with vehicle, OB and MEN 10207. The white arrow heads indicated the disseminated cancer cells. Scale bar: 100 μm. ( E ) Quantitative analysis of D showed the relative fluorescence intensity of GFP in vivo. The measurement of fluorescence of GFP at 24 hpi was used as the baseline. n = 10. P = 0.0012 (Vehicle vs. OB), P = 0.0003 (Vehicle vs. MEN 10207). ( F ) Quantification analysis of the migrated distance of brain metastatic cells along PceV in zebrafish at 72 hpi was conducted. n = 10. P = 1.1 × 10 −6 (Vehicle vs. OB), P = 8.6 × 10 −7 (Vehicle vs. MEN 10207). ( G ) Schematic representation illustrates the nude mice xenograft model using MDA-MB-231 (GFP + ) cells. ( H ) Representative light-sheet fluorescence microscopy images of whole brains of nude mice xenograft models treated with vehicle, and MEN 10207. Scale bar: 1000 μm. ( I ) Quantitative analysis of H showed the brain metastatic tumor volume in vivo. n = 3. ( J ) Immunofluorescence staining (scale bar: 100 μm) for CD31 (red signal) and DAPI (blue signal) was conducted on mice brain section and three-dimensional imaging (scale bar: 50 μm) was performed to visualize the interaction between MDA-MB-231 cells and the blood vessels in mouse brain. MDA-MB-231 cells are displayed in green. The white arrow heads indicated the disseminated cancer cells in the mice brain. ( K ) MDA-MB-231 cells treated with vehicle and MEN 10207 were subjected to western blot assay to analyze the protein level of activated GTP-bound CDC42, following Reelin treatment. ( L ) Quantitative analysis revealed the relative GTP-bound CDC42 level normalized to total CDC42 protein. n = 5. Data information: data are shown as mean ± SD, P values were analyzed with unpaired Student’s t test ( I , vehicle-treated group vs. MEN 10207-treated group) and one-way ANOVA test ( E , F , L ). ns: non-significant. .

Article Snippet: Rabbit anti-LRP8 antibody , Boster , #A03444-2.

Techniques: Binding Assay, Confocal Microscopy, Fluorescence, In Vivo, Microscopy, Immunofluorescence, Staining, Imaging, Western Blot