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Image Search Results
Journal: Oncogene
Article Title: Identification of a novel EphB4 phosphodegron regulated by the autocrine IGFII/IR A axis in malignant mesothelioma
doi: 10.1038/s41388-019-0854-y
Figure Lengend Snippet: List of antibodies and related reagents
Article Snippet: Ubiquitin, human synthetic , n/a , 800
Techniques: Concentration Assay, Ubiquitin Proteomics
Journal: The Journal of Experimental Medicine
Article Title: STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets
doi: 10.1084/jem.20201329
Figure Lengend Snippet: CCR8 expression in tumor T reg cells and characterization of anti-CCR8 antibodies . (A) Representative histogram plot depicting expression of CCR8 on 4-1BB + and 4-1BB − CD4 + Foxp3 − T EFF population in MC38 tumors. (B) Representative histogram plot depicting expression of CCR8 on CCR7 + and CCR7 − CD8 + T cells in MC38 tumors. (C) Flow cytometry analysis of tumor-infiltrating T reg cells across multiple mouse tumor models shown as representative FACS plots (left) and frequencies in tumors and peripheral lymphoid organs (right). *, P = 0.0250; ****, P < 0.0001; two-way ANOVA with Tukey’s post-hoc analysis. (D) Flow cytometry analysis of CCR8 expression on immune cell types (Foxp3 + T reg cells, Foxp3 − T effectors, CD8 + , NKp46 + NK, CD19 + B, and CD11b + ) in tumors and peripheral lymphoid tissues across tumor models. (E) In vitro chemotaxis assay evaluating the ability of CCR8-depleting and -nondepleting antibodies to block 100 pM CCL1-induced chemotaxis. (F) Bar graph comparing IC 50 values from the chemotaxis assay testing CCR8-depleting and -nondepleting antibodies ( n = 6). (G) Pharmacokinetic assessment of serum antibody concentration after single-dose administration of CCR8-depleting and -nondepleting antibodies (10 mg/kg i.p.). (H) Serum concentration at 72 h of animals treated as in G. DLN, draining LN; SPL, spleen; MFI, mean fluorescence intensity.
Article Snippet:
Techniques: Expressing, Flow Cytometry, In Vitro, Chemotaxis Assay, Blocking Assay, Concentration Assay, Fluorescence