Journal: Cell

Article Title: Structure and Function of the Nuclear Pore Complex Cytoplasmic mRNA Export Platform

doi: 10.1016/j.cell.2016.10.028

Figure Lengend Snippet:

Article Snippet: MATa ura3-52 his3-Δ200 trp1-1 leu2-3,112 lys2-801 NUP145cΔ(aa317-326)-ProteinA::HIS5 NUP82-GFP-3xFlag-6xHis::klURA3 , This study , Nup145c(1-316)-(327-712)/ Nup82-GFP3xF6xH.

Techniques: Purification, Recombinant, Electron Microscopy, Staining, Sequencing, Modification, Microscopy, Filtration, Extraction, Structural Proteomics, Mass Spectrometry, Software

Journal: Scientific Reports

Article Title: EndophilinAs regulate endosomal sorting of BDNF-TrkB to mediate survival signaling in hippocampal neurons

doi: 10.1038/s41598-017-02202-4

Figure Lengend Snippet: TrkB receptors accumulate in EEA1 and Rab7 positive endosomes in EndophilinA triple knockouts. ( A ) HA-conjugated ProteinA beads (or IgG control beads) were used to pull down HA-TrkB (left) and EndophilinA1, A2, or A3-GFP (right) in co-transfected Hek293 cells in the presence or absence of 100 ng/ml BDNF. ( B ) Representative images of wild-type and EndophilinA TKO mouse neuronal cultures transfected with TrkB-GFP and the RFP-tagged endosomal markers EEA1 or Rab7. Arrows indicate TrkB colocalizing with EEA1 or Rab7. Scale bar = 10 µm. ( C ) Quantitation of colocalization of TrkB-GFP with RFP-tagged EEA1 or Rab7 ( D ), in wild-type and EndophilinA TKO neurons. In EndophilinA triple knockout neurons, more TrkB was found in EEA1 and Rab7 positive endosomes in both the presence and absence of BDNF compared to control; n = 30 images from 4 independent cultures, significance determined by unpaired two-tailed Student’s t-test compared to non-stimulated control and between non stimulated TKO and BDNF treated TKO, error = SEM; **p < 0.01, ***p < 0.001.

Article Snippet: Figure 6 TrkB receptors accumulate in EEA1 and Rab7 positive endosomes in EndophilinA triple knockouts. ( A ) HA-conjugated ProteinA beads (or IgG control beads) were used to pull down HA-TrkB (left) and EndophilinA1, A2, or A3-GFP (right) in co-transfected Hek293 cells in the presence or absence of 100 ng/ml BDNF. ( B ) Representative images of wild-type and EndophilinA TKO mouse neuronal cultures transfected with TrkB-GFP and the RFP-tagged endosomal markers EEA1 or Rab7.

Techniques: Control, Transfection, Quantitation Assay, Triple Knockout, Two Tailed Test