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Merck KGaA
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Image Search Results
Journal: ACS Omega
Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
doi: 10.1021/acsomega.3c01117
Figure Lengend Snippet: Agarose gel image (0.8%) showing Lane 1: 2 Log DNA ladder, Lane 2: pET-32a_GSAP_I4 plasmid at 6.6 kb, Lane 3: Linearized pET-32a_GSAP_I4 plasmid digested after restriction digestion using BamHI restriction enzyme, and Lane 4: pET-32a vector at 5.9 kb and GSAP_I4 at 0.7 kb after double digestion using BamHI and EcoRI restriction enzymes. (Uncropped image of this agarose gel is shown in Supporting Material; Figure S3 ).
Article Snippet: The refolded and
Techniques: Agarose Gel Electrophoresis, Plasmid Preparation
Journal: ACS Omega
Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
doi: 10.1021/acsomega.3c01117
Figure Lengend Snippet: (a) SDS-PAGE gel image showing Trx-GSAP_I4 protein expression and purification from inclusion bodies. Lane 1: Protein marker; Lane 2: Crude sample; Lane 3: Flow through; Lane 4–5: Wash; Lane 6–10: Purified Trx-GSAP_I4 protein. (b) SDS-PAGE gel image showing Lane 1: Protein marker; Lane 2: Dialyzed Trx-GSAP_I4 (44 kDa); Lane 3: Cleaved GSAP_I4 (30 kDa) and Trx (12 kDa) after tag removal using thrombin protease; Lane 4: FPLC purified GSAP_I4 (∼30 kDa) (repeated SDS-PAGE images for the above purifications are shown in Supporting Material; Figure S5 ).
Article Snippet: The refolded and
Techniques: SDS Page, Expressing, Purification, Marker
Journal: ACS Omega
Article Title: To Explore the Binding Affinity of Human γ-Secretase Activating Protein (GSAP) Isoform 4 with APP-C99 Peptides
doi: 10.1021/acsomega.3c01117
Figure Lengend Snippet: Image showing (a) CD spectra for denatured Trx_GSAP_I4. (b) CD spectra for refolded cleaved GSAP_I4. (c) Western blot for Trx_GSAP_I4 using anti-PION primary antibody. The orange triangle and blue triangle represent 50 and 37 kDa mark, respectively.(Uncropped image for the western blot is shown in Supporting Material; Figure S7 ).
Article Snippet: The refolded and
Techniques: Western Blot