Journal: International Journal of Molecular Sciences

Article Title: MMP9 Differentially Regulates Proteins Involved in Actin Polymerization and Cell Migration during TGF-β-Induced EMT in the Lens

doi: 10.3390/ijms222111988

Figure Lengend Snippet: Table showing fold changes of cytoskeletal protein expression. Cytoskeletal protein array analysis was conducted using untreated wildtype (control), wildtype treated with 500 pg/mL TGF-β for 72 h (TG), untreated MMP9KO (un-MMP9KO) and MMP9KO treated with 500 pg/mL TGF-β for 72 h (MMP9KO-TG) mouse LECs ( n = 3 independent experiments, where 10 µg of protein per treatment was used for each experiment). Red represents upregulation, green represents downregulation and white indicates that no notable difference was observed between the two compared treatment groups. A darker shade of the color of the box indicates a greater fold difference between the two compared treatment groups.

Article Snippet: To demonstrate the role of MMP9 in modulating the expression and activation of proteins required during actin polymerization and reorganization, we performed a cytoskeletal protein array (Fullmoon Biosystems) using untreated and TGF-β-treated wildtype and MMP9KO LECs.

Techniques: Expressing, Protein Array, Control

Journal: International Journal of Molecular Sciences

Article Title: MMP9 Differentially Regulates Proteins Involved in Actin Polymerization and Cell Migration during TGF-β-Induced EMT in the Lens

doi: 10.3390/ijms222111988

Figure Lengend Snippet: Graphs showing the average signal of protein expression for selected proteins. Cytoskeletal protein array analysis was conducted using LEC explants from wildtype (control) and MMP9KO mice were left untreated (un-MMP9KO) or with treated with 500 pg/mL TGF-β for 72 h (MMP9KO-TG). Cortactin, focal adhesion kinase (FAK), lim-domain kinase-1 (LIMK1) and myosin light chain-2 (MLC2) were analyzed. Data was normalized to the median GAPDH and then averaged. A 2-way ANOVA with multiple comparisons was performed and the data was graphed using Graphpad Prism. Error bars indicate ± standard deviation ( n = 3 independent experiments, where 10 µg of protein per treatment was used for each experiment; ** p < 0.01 **** p < 0.0001). For Cortactin and FAK, p < 0.0001 across all treatment groups i.e., WT vs. TG or un-MMP9KO or MMP9KO-TG, TG vs. un-MMP9KO or MMP9KO-TG and un-MMP9KO vs. MMP9KO-TG. For LIMK1, p < 0.0001 WT vs. other treatments; p < 0.01 TG vs. un-MMP9KO and un-MMP9KO vs. MMP9KO-TG. For MLC2, p < 0.0001 WT vs. TG, TG vs. un-MMP9KO and TG vs. MMP9KO-TG.

Article Snippet: To demonstrate the role of MMP9 in modulating the expression and activation of proteins required during actin polymerization and reorganization, we performed a cytoskeletal protein array (Fullmoon Biosystems) using untreated and TGF-β-treated wildtype and MMP9KO LECs.

Techniques: Expressing, Protein Array, Control, Standard Deviation