Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prosaposin orchestrates a TGFβ1-driven paracrine loop between Schwann cells and gastric cancer to accelerate perineural invasion

doi: 10.1186/s13046-026-03652-3

Figure Lengend Snippet: Bidirectional PSAP-TGFβ1 Paracrine Signaling Sustains SCs–GC Crosstalk. A Schematic of paracrine communication between SCs and GC Cells. B The secretion of PSAP by GC cells increased after co-culture with SCs. C TGFβ1 expression was increased in SCs co-cultured with GC cells and treated with rPSAP. D TGFβ1 secretion was increased in SCs co-cultured with GC cells and treated with rPSAP. E – F The secretion and expression of PSAP in GC cells treated with rTGFβ1 were increased. G - H The TGFβ1 inhibitor (Asiaticoside) can inhibit the effect of rTGFβ1 on the secretion and expression of PSAP

Article Snippet: Secreted PSAP and TGFβ1 levels in cell supernatants were quantified using Human PSAP (CUSABIO, China, #CSB-E12837h) and Rat TGFβ1 (JIANGLAI BIO, China, # JL12342,) ELISA kits.

Techniques: Co-Culture Assay, Expressing, Cell Culture

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prosaposin orchestrates a TGFβ1-driven paracrine loop between Schwann cells and gastric cancer to accelerate perineural invasion

doi: 10.1186/s13046-026-03652-3

Figure Lengend Snippet: PSAP Activates GPR37/RAC1/ACTB Signaling to Drive TGFβ1 Secretion in SCs. A Schematic representation of PSAP secreted by GC cells acting on SCs. B-D rPSAP enhances SCs proliferation, migration, invasion and clonogenic capacity. E Functional enrichment analysis showed that the cytoskeleton of SCs changed after co-culture significantly. F Phalloidin staining reveals cytoskeletal changes in SCs following co-culture and rPSAP treatment. G SPR demonstrated direct binding between PASP and GPR37. H SCs co-cultured and exposed to rPSAP significantly activated GPR37/RAC1/ACTB signaling axis and TGFβ1 expression. I-K GPR37 sustains RAC1–ACTB signaling to drive TGF-β1 production and SC aggressiveness, conferring responsiveness to rPSAP

Article Snippet: Secreted PSAP and TGFβ1 levels in cell supernatants were quantified using Human PSAP (CUSABIO, China, #CSB-E12837h) and Rat TGFβ1 (JIANGLAI BIO, China, # JL12342,) ELISA kits.

Techniques: Migration, Functional Assay, Co-Culture Assay, Staining, Binding Assay, Cell Culture, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prosaposin orchestrates a TGFβ1-driven paracrine loop between Schwann cells and gastric cancer to accelerate perineural invasion

doi: 10.1186/s13046-026-03652-3

Figure Lengend Snippet: TGFβ1 Engages the TGFβ1/Smad4/Sortilin Axis to Amplify PSAP Secretion and PNI. A Schematic representation of TGFβ1 secreted by SCs acting on GC cells. B In vitro PNI assays showed that downregulation of SCs-derived TGF-β1 diminished GC cell PNI. C-E Asiaticoside can inhibit the effects of rTGFβ1 on the proliferation, invasion and PSAP secretion of GC cells. F Schematic representation of PSAP secretion. G TGFβ1/Smad4 signaling activation induces increased PSAP secretion through the suppression of Sortilin expression. H Dual-luciferase and ChIP-qPCR analyses showed that Smad4 binds the Sortilin promoter and represses its transcription, linking TGF-β1/Smad4 activation to Sortilin downregulation. I Sortilin overexpression suppresses PSAP secretion

Article Snippet: Secreted PSAP and TGFβ1 levels in cell supernatants were quantified using Human PSAP (CUSABIO, China, #CSB-E12837h) and Rat TGFβ1 (JIANGLAI BIO, China, # JL12342,) ELISA kits.

Techniques: In Vitro, Derivative Assay, Activation Assay, Expressing, Luciferase, ChIP-qPCR, Over Expression

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prosaposin orchestrates a TGFβ1-driven paracrine loop between Schwann cells and gastric cancer to accelerate perineural invasion

doi: 10.1186/s13046-026-03652-3

Figure Lengend Snippet: Therapeutic Targeting of PSAP/TGFβ1 Suppresses PNI In Vivo. A Inhibition of PSAP/TGFβ1 reduces PNI Incidence DRG-PNI model in vitro . B Schematic of a Sciatic Nerve Invasion Murine Model. C-D Tumor formation and H&E and IHC detection in the sciatic nerve model. E – F Functional and physiological assessments post intervention (Sciatic Nerve Score, Body Weight). G GMulticolor IF was used to verify the spatial distribution of PSAP, TGFβ1 and SCs(S100β) in PNI-positive tumor tissues

Article Snippet: Secreted PSAP and TGFβ1 levels in cell supernatants were quantified using Human PSAP (CUSABIO, China, #CSB-E12837h) and Rat TGFβ1 (JIANGLAI BIO, China, # JL12342,) ELISA kits.

Techniques: In Vivo, Inhibition, In Vitro, Functional Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Prosaposin orchestrates a TGFβ1-driven paracrine loop between Schwann cells and gastric cancer to accelerate perineural invasion

doi: 10.1186/s13046-026-03652-3

Figure Lengend Snippet: PSAP/TGFβ1/S100β multimodal signatures correlate with PNI and improve PNI discrimination. A IHC Staining of PSAP, TGFβ1, and S100β in PNI Subgroups. B Kaplan–Meier Survival Analysis by PNI Status in a Clinical Cohort. C Nomogram of PSAP, TGFβ1 and S100β combined to predict PNI. D - F ROC curve analysis, DCA, and calibration curves for comparison of PTS model and Baseline model for predicting PNI status. G The PTS model performance SHAP analysis. H - I ROC and calibration curves for the PTS and baseline models in an independent validation cohort. J Reclassification analysis of the PTS model and Baseline model

Article Snippet: Secreted PSAP and TGFβ1 levels in cell supernatants were quantified using Human PSAP (CUSABIO, China, #CSB-E12837h) and Rat TGFβ1 (JIANGLAI BIO, China, # JL12342,) ELISA kits.

Techniques: Immunohistochemistry, Comparison, Biomarker Discovery

Journal: Journal of Molecular Neuroscience

Article Title: Prosaposin Reduces α-Synuclein in Cells and Saposin C Dislodges it from Glucosylceramide-enriched Lipid Membranes

doi: 10.1007/s12031-022-02066-y

Figure Lengend Snippet: Antibodies used for Western blot

Article Snippet: PSAP-GFP (HG16224-ACG, Sino Biological) and control-EGFP (13031, Addgene) plasmids underwent amplification in E. coli and were purified using plasmid extraction kits (12143, Qiagen).

Techniques: Western Blot

Journal: Journal of Molecular Neuroscience

Article Title: Prosaposin Reduces α-Synuclein in Cells and Saposin C Dislodges it from Glucosylceramide-enriched Lipid Membranes

doi: 10.1007/s12031-022-02066-y

Figure Lengend Snippet: Overexpression of Prosaposin (PSAP) lowers levels of α-synuclein. ( a ) Representative Western Blot for anti-GFP (Left) and anti-PSAP (Right) in EGFP-Overexpressing (EGFP-OE) cells and PSAP-Overexpressing (PSAP-OE) cells showing PSAP-GFP at 105 kDa, endogenous PSAP at 70 kDa, and control EGFP at 32 kDa. ( b ) Representative live-cell images of PSAP-OE cells for PSAP-GFP co-localized with the lysosome (Green: PSAP-GFP, Magenta: LysoTracker, scale bar 5 µm). ( c ) Representative Western Blot for anti-α-synuclein and anti-β-actin in EGFP-OE cells and PSAP-OE cells. ( d ) Quantification of Western Blot analysis showing levels of α-synuclein in EGFP-OE cells (n = 10) and PSAP-OE cells (n = 8). ( e ) Real-time PCR analysis showing mRNA levels of SNCA in EGFP-OE cells (n = 6) and PSAP-OE cells (n = 6). ( f ) Quantification of α-synuclein levels in cell culture media of EGFP-OE cells (n = 8) and PSAP-OE cells (n = 8) measured by enzyme-linked immunosorbent assay. ( g ) Representative Western blot for anti-Glucocerebrosidase (GCase) and anti-GAPDH in EGFP-OE cells and PSAP-OE cells. ( h ) Quantification of Western blot analysis showing levels of GCase in EGFP-OE cells (n = 8) and PSAP-OE cells (n = 8). ( i ) Non-specific GBA activity and specific GCase activity analysis in EGFP-OE cells (n = 8) and PSAP-OE cells (n = 8) treated with or without AMP-deoxynojirimycin. Data are expressed as mean ± SEM. n.s: non-significant, p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001, Mann–Whitney U test (d, e, f, h,) and Two-way ANOVA with Tukey’s multiple comparison test (i)

Article Snippet: PSAP-GFP (HG16224-ACG, Sino Biological) and control-EGFP (13031, Addgene) plasmids underwent amplification in E. coli and were purified using plasmid extraction kits (12143, Qiagen).

Techniques: Over Expression, Western Blot, Real-time Polymerase Chain Reaction, Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay, MANN-WHITNEY

Journal: Physiological Reports

Article Title: Endothelin inhibits renin release from juxtaglomerular cells via endothelin receptors A and B via a transient receptor potential canonical‐mediated pathway

doi: 10.14814/phy2.12240

Figure Lengend Snippet: RT‐PCR for ETA (top) and ETB (bottom) identifying bands at 260 and 231 bp, respectively. Column 1 is the “no‐template” negative control. Column 2 is total mRNA obtained from isolated juxtaglomerular (JG) cells. Column 3 is total mRNA obtained from the positive control of liver. Column 4 is the calibration scale (100 bp ladder).

Article Snippet: Afterward, slides were incubated for 1 h at 37°C with a 1:50 dilution of an antibody against mouse ETA or ETB receptor protein (Alomone, Jerusalem, Israel), and then for 1 h at 37°C with a 1:100 dilution of secondary antibody (Alexa Fluor 568 goat antimouse IgG; Life Technologies, Grand Island, NY).

Techniques: Reverse Transcription Polymerase Chain Reaction, Negative Control, Isolation, Positive Control

Journal: Physiological Reports

Article Title: Endothelin inhibits renin release from juxtaglomerular cells via endothelin receptors A and B via a transient receptor potential canonical‐mediated pathway

doi: 10.14814/phy2.12240

Figure Lengend Snippet: Immunofluorescence of a single juxtaglomerular (JG) cell using two antibodies; one specific for renin (in green) to confirm this is a JG cell, and another specific for the ETA (top) and ETB (bottom) receptors isoforms (in red). The third panel shows both renin and A1R in the same JG cell (merged image).

Article Snippet: Afterward, slides were incubated for 1 h at 37°C with a 1:50 dilution of an antibody against mouse ETA or ETB receptor protein (Alomone, Jerusalem, Israel), and then for 1 h at 37°C with a 1:100 dilution of secondary antibody (Alexa Fluor 568 goat antimouse IgG; Life Technologies, Grand Island, NY).

Techniques: Immunofluorescence

Journal: Physiological Reports

Article Title: Endothelin inhibits renin release from juxtaglomerular cells via endothelin receptors A and B via a transient receptor potential canonical‐mediated pathway

doi: 10.14814/phy2.12240

Figure Lengend Snippet: Renin release from juxtaglomerular (JG) cells under basal conditions (Control), after incubation with ET‐1, or ET‐1 plus different concentrations of the ETB receptor antagonist BQ788 (3 μ mol/L and 10 μ mol/L). Incubation with ET‐1 reduced renin release. This response was inhibited by 10 μ mol/L of an ETB receptor blocker. * P < 0.05 versus control.

Article Snippet: Afterward, slides were incubated for 1 h at 37°C with a 1:50 dilution of an antibody against mouse ETA or ETB receptor protein (Alomone, Jerusalem, Israel), and then for 1 h at 37°C with a 1:100 dilution of secondary antibody (Alexa Fluor 568 goat antimouse IgG; Life Technologies, Grand Island, NY).

Techniques: Incubation