Journal: Fungal biology

Article Title: Morphological changes in melanized and non-melanized Cryptococcus neoformans cells post exposure to sparsely and densely ionizing radiation demonstrate protective effect of melanin.

doi: 10.1016/j.funbio.2017.08.010

Figure Lengend Snippet: Fig. 5. Micrographs of CN cells obtained with fluorescence microscopy after treatment with maximum doses of alpha particle or deuteron irradiation relative to sham-irradiated. Following exposure cells were fixed with formaldehyde prior to staining with propidium iodide and digestion with RNAse to specifically stain the DNA.

Article Snippet: Samples were then transferred into a propidium iodide/RNAse A solution (Novus, Littleton, CO) and stained for at least 30 min prior to imaging.

Techniques: Microscopy, Irradiation, Staining

Journal: Microarrays

Article Title: Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

doi: 10.3390/microarrays5020008

Figure Lengend Snippet: Representative time traces of fluorescent signals monitored in the polarity Sensor for Viability and Apoptosis (pSIVA) ( A ) and propidium iodide (PI) ( B ) channels. A few typical traces are highlighted in green and red, respectively. The first apoptotic events are observed after 3 h whereas the majority of induced cell deaths occur after 20 h. The decrease in the pSIVA signal at late times can be attributed to bleaching of the fluorophores. Fluorescent signals are background corrected.

Article Snippet: Cells were stained with polarity Sensor for Viability and Apoptosis (pSIVA-IANBD, Novus Biologicals) [ , ] (the stock solution was diluted 1:50) and 1 μ M propidium iodide (Novus Biologicals).

Techniques: