Journal: Histochemistry and Cell Biology

Article Title: The trophoblast plug during early pregnancy: a deeper insight

doi: 10.1007/s00418-016-1474-z

Figure Lengend Snippet: Antibodies used in immunohistochemistry

Article Snippet: Matrix metalloproteinase 1 (MMP-1) , Protein Tech (Rosemont, USA) , 1:500 , Rabbit IgG pc.

Techniques: Immunopeptidomics

Journal: Histochemistry and Cell Biology

Article Title: The trophoblast plug during early pregnancy: a deeper insight

doi: 10.1007/s00418-016-1474-z

Figure Lengend Snippet: MMP-1 expression in arterial trophoblast plugs. Immunohistochemical single ( a , c , e , g ) and double ( b , d , f , h ) staining of serial sections ( a – b , c – d , e – f , g – h ) of invaded decidua basalis (gestational age 11 weeks a – d and gestational week 7 e – h ) for matrix metalloproteinase 1 (MMP-1) and for major histocompatibility complex, class I, G (HLA-G) ( brown , serves as marker for extravillous trophoblasts) together with von Willebrand factor (vWF) ( blue , serves as marker for vascular endothelial cells). Nuclei are counterstained with Haemalum ( a , c , e , g ) or no nuclear counterstain ( b , d , f , h ). a , c , e , g Endovascular trophoblasts within trophoblast plugs ( asterisk ) are clearly positive for MMP-1. Additionally interstitial trophoblasts as well as decidual stroma cells are positive for MMP-1. b , d , f , h Immunohistochemical double staining in serial sections enables a clear classification of the assessed structures and cells and clearly confirms the extravillous origin of the trophoblasts composing the trophoblast plug. Scale bar 50 µm

Article Snippet: Matrix metalloproteinase 1 (MMP-1) , Protein Tech (Rosemont, USA) , 1:500 , Rabbit IgG pc.

Techniques: Expressing, Immunohistochemical staining, Staining, Immunopeptidomics, Marker, Double Staining