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Abnova
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Yagai Corp
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AnaSpec
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AstraZeneca ltd
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Blackwell Verlag
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MBL Life science
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Enzo Biochem
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Diabor Pty Ltd
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FUJIFILM
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Image Search Results
Journal: Reproductive Medicine and Biology
Article Title: Identification of complexes of gelatinase A and tissue inhibitor of metalloproteinase‐2 in human follicular fluid
doi: 10.1046/j.1445-5781.2003.00029.x
Figure Lengend Snippet: Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE), gelatin‐zymography and western blot analysis of pooled the fractions from gelatin‐cellulofine column chromatography. The concentrated sample (tubes 68–73) from gelatin‐cellulofine column chromatography was electrophoresed in 12% SDS‐PAGE (a), 12% gelatin‐zymography (b–d) and western blot analysis (e–g). (a) Reducing concentrated sample (silver stain), (b) non‐reducing mixture of standard human progelatinase B (proMMP‐9), progelatinase A (proMMP‐2) and gelatinase A (MMP‐2), (c) non‐reducing concentrated sample, (d) non‐reducing concentrated sample with EDTA, (e) reducing concentrated sample against antihuman MMP‐2 antibody, (f) reducing standard human tissue inhibitor of metalloproteinase (TIMP‐2) against antihuman TIMP‐2 antibody, and (g) reducing concentrated sample against antihuman TIMP‐2 antibody.
Article Snippet: Standard proMMP‐9,
Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Zymography, Western Blot, Column Chromatography, Silver Staining