Journal:

Article Title: Curcumin (Diferuloylmethane) Inhibits Cell Proliferation, Induces Apoptosis, and Decreases Hormone Levels and Secretion in Pituitary Tumor Cells

doi: 10.1210/en.2007-1760

Figure Lengend Snippet: Curcumin decreases intracellular levels and release of PRL and GH. A, GH3 or MMQ cells were treated with the indicated doses of curcumin (Cur) for 48 h, and equal amounts of cell lysates were subjected to Western blotting with anti-rPRL Ab or antirat GH Ab (upper panels). Equal loading was confirmed by determining actin levels (lower panels). Data are from a single experiment that is representative of at least three independent experiments for each cell line. B, Fold change in intracellular hormone levels, detected in A, were quantitated as described in Materials and Methods. Data are mean ± sem of three independent experiments. *, Significant differences (P < 0.05) from control values. C, GH3 or MMQ cells were treated with the indicated doses of curcumin for 48 h, and PRL and GH released into the CM were determined by EIA as described in Materials and Methods. PRL/GH release was calculated as nanograms PRL or GH per milliliter of CM and is expressed as percent of control. Data are mean ± sem of three to four independent experiments. *, Significant differences (P < 0.05) from control values.

Article Snippet: Determination of PRL and GH content in conditioned medium GH3 and MMQ cells were treated with the indicated amount of curcumin for 48 h, and 100 μl of conditioned medium (CM) were collected for each treatment and stored at 4 C. PRL levels in CM from GH3 and MMQ cells as well as GH levels in CM from GH3 cells were determined by using a rat PRL EIA kit (Alpco Diagnostics) or rat GH EIA kit (Alpco Diagnostics) as per the manufacturer’s instructions.

Techniques: Western Blot, Control

Journal: Cancer research

Article Title: Prolactin promotes fibrosis and pancreatic cancer progression

doi: 10.1158/0008-5472.CAN-18-3064

Figure Lengend Snippet: (A) PRLR is detected in PANC-1 but not in BxPC-3 pancreatic cell lines. Lysates from T47D breast cancer cell line was used as positive control for PRLR detection. (B) Prolactin can stimulate proliferation while silencing of PRLR even in the presence of prolactin leads to a decline in the number of cells. Schematic illustration of known oncogenic pathways activated by PRL/PRLR axis. (C) Both STAT3 and STAT5 are activated in the PDAC cell lines. (D) Western blot analyses of Serum-starved PANC-1 cells that had been cultured with 100ng/ml hrPRL for up to 60 minutes. (E) Luciferase assay on PANC-1 cells transfected with STAT5 reporter and stimulated with the increasing amount of rhPRL as indicated.

Article Snippet: Recombinant human prolactin was obtained from R&D Systems Inc. (Minneapolis, MN).

Techniques: Positive Control, Western Blot, Cell Culture, Luciferase, Transfection