Journal: RSC Advances

Article Title: Retracted Article: Long non-coding RNA PCAT1 facilitates cell growth in multiple myeloma through an MTDH-mediated AKT/β-catenin signaling pathway by sponging miR-363-3p †

doi: 10.1039/c9ra06188f

Figure Lengend Snippet: PCAT1 knockdown repressed cell proliferation by inducing cell cycle arrest and promoted cell apoptosis in OPM2 and U266B1 cells. (A) The level of PCAT1 in nPCs, MM1S, RPMI-8226, OPM2 and U266B1 cells was tested by qRT-PCR. (B–I) The OPM2 and U266B1 cells were transfected with sh-PCAT1 or sh-NC. (B) The level of PCAT1 in transfected cells was detected via qRT-PCR. (C and D) The cell viability of transfected OPM2 and U266B1 cells was monitored by MTT assay. (E and F) The G1 and S phase fractions in transfected cells were evaluated through flow cytometry. (G) The apoptotic rate in transfected cells was analyzed via flow cytometry. (H and I) The protein levels of cyclin D1, c-Myc, Bax and Bcl-2 were detected by western blot assay. * P < 0.05.

Article Snippet: Three MM cell lines, RPMI-8226, OPM2 and U266B1, were bought from Huzhen (Shanghai, China), another MM cell line, MM1S, was purchased from Tongpai (Shanghai, China) and neural progenitor cells (nPCs) were obtained from Hopstem (Hangzhou, China).

Techniques: Knockdown, Quantitative RT-PCR, Transfection, MTT Assay, Flow Cytometry, Western Blot

Journal: RSC Advances

Article Title: Retracted Article: Long non-coding RNA PCAT1 facilitates cell growth in multiple myeloma through an MTDH-mediated AKT/β-catenin signaling pathway by sponging miR-363-3p †

doi: 10.1039/c9ra06188f

Figure Lengend Snippet: MTDH depletion blocked cell proliferation, as well as promoting G1/S ratio and cell apoptosis in OPM2 and U266B1 cells. (A) The protein level of MTDH in nPCs, MM1S, RPMI-8226, OPM2 and U266B1 cells was tested by western blot assay. (B–I) The OPM2 and U266B1 cells were transfected with sh-MTDH or sh-NC. (B) The protein level of MTDH in transfected cells was detected via western blot assay. (C and D) The cell viability of transfected OPM2 and U266B1 cells was monitored by MTT assay. (E and F) The G1 and S phase fractions in transfected cells were evaluated through flow cytometry. (G) The apoptotic rate in transfected cells was analyzed via flow cytometry. (H and I) The protein levels of cyclin D1, c-Myc, Bax, and Bcl-2 were detected by western blot assay. * P < 0.05.

Article Snippet: Three MM cell lines, RPMI-8226, OPM2 and U266B1, were bought from Huzhen (Shanghai, China), another MM cell line, MM1S, was purchased from Tongpai (Shanghai, China) and neural progenitor cells (nPCs) were obtained from Hopstem (Hangzhou, China).

Techniques: Western Blot, Transfection, MTT Assay, Flow Cytometry

Journal: Journal of Inherited Metabolic Disease

Article Title: Metabolomics analysis of antiquitin deficiency in cultured human cells and plasma: Relevance to pyridoxine‐dependent epilepsy

doi: 10.1002/jimd.12569

Figure Lengend Snippet: Western blot analysis showing antiquitin and β‐Actin expression in control (untransfected) and CRISPR/Cas transfected colonies of neuronal progenitor cells (NPCs) following single cell expansion

Article Snippet: ReNcell CX human neuronal progenitor cells (NPCs) were transfected with Cas9 protein and guide RNA (LabOmics, Belgium) targeting ALDH7A1 , using the Neon nucleofector (Life Technologies) according to the manufacturer's protocol.

Techniques: Western Blot, Expressing, Control, CRISPR, Transfection

Journal: Journal of Inherited Metabolic Disease

Article Title: Metabolomics analysis of antiquitin deficiency in cultured human cells and plasma: Relevance to pyridoxine‐dependent epilepsy

doi: 10.1002/jimd.12569

Figure Lengend Snippet: Significantly altered metabolites in antiquitin‐deficient neuronal progenitor cells (NPCs) compared to control NPCs (A) cultured in high‐lysine containing media; (B) cultured in lysine‐free and high pyridoxine (PN) media

Article Snippet: ReNcell CX human neuronal progenitor cells (NPCs) were transfected with Cas9 protein and guide RNA (LabOmics, Belgium) targeting ALDH7A1 , using the Neon nucleofector (Life Technologies) according to the manufacturer's protocol.

Techniques: Control, Cell Culture

Journal: Journal of Inherited Metabolic Disease

Article Title: Metabolomics analysis of antiquitin deficiency in cultured human cells and plasma: Relevance to pyridoxine‐dependent epilepsy

doi: 10.1002/jimd.12569

Figure Lengend Snippet: Significantly altered features in antiquitin‐deficient neuronal progenitor cells (NPCs) incubated in pyridoxine (PN)‐supplemented media, compared to cells cultured in media without supplementation

Article Snippet: ReNcell CX human neuronal progenitor cells (NPCs) were transfected with Cas9 protein and guide RNA (LabOmics, Belgium) targeting ALDH7A1 , using the Neon nucleofector (Life Technologies) according to the manufacturer's protocol.

Techniques: Incubation, Cell Culture