Journal: Journal of Cellular and Molecular Medicine

Article Title: Stephanine Protects Against Osteoporosis by Suppressing Osteoclastogenesis via Inhibition of the RANKL — RANK Interaction

doi: 10.1111/jcmm.70256

Figure Lengend Snippet: SA prevents OVX‐induced bone loss in mice by inhibiting osteoclastogenesis. (A) H&E staining (original magnification ×100) and TRAP staining (original magnification ×400) of the distal femur sections in each group. Representative images are displayed. (B) The trabecular bone area in the femur was quantified. (C) The number of TRAP‐positive cells/field was calculated. (D–I) The serum levels of TRACP‐5b (D), CTX‐I (E), RANKL (F), OCN (G), PINP (H) and OPG (I) were determined using the corresponding ELISA kits. Data are expressed as the mean ± SEM ( n = 10). * p < 0.05 and *** p < 0.001 versus the sham group; # p < 0.05 and ## p < 0.01 versus the OVX group.

Article Snippet: Mouse TRACP‐5b, osteocalcin (OCN), c‐telopeptide of type I collagen (CTX‐I), and procollagen I N‐terminal peptide (PINP) ELISA kits were sourced from Cusabio Technology (Houston, TX, USA).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: Pla2g7 regulates bone homeostasis via Alox12/12-HETE/Gpr31 signaling axis

doi: 10.1038/s41467-025-66285-8

Figure Lengend Snippet: A The diagrams of bone mass in Pla2g7 f/f , Pla2g7 LysM and Pla2g7 CTSK female mice (scale bar, 1 mm). B , C Trabecular-related parameters in Pla2g7 f/f , Pla2g7 LysM and Pla2g7 CTSK female mice, including BV/TV, Tb.Th, Tb.N and Tb.Sp. n = 6 biologically independent mice per group. D – G HE and TRAP staining of distal of femur, and the histomorphometric parameters of osteoclasts in each group, including Oc.N/BS and Oc.S/BS. The bottom image enlarges the black box labeled region of the middle image. n = 6 biologically independent mice per group. (scale bar, normal images, 200 μm, enlarged images, 100 μm). H , I The expression of serum CTX and PINP using Elisa assays. n = 6 biologically independent mice per group. J The body and hindlimbs length in Pla2g7 f/f , Pla2g7 LysM and Pla2g7 CTSK female mice. K , L The rescued-condition of Pla2g7 conditional knockout in OVX-induced bone loss. OVX is performed at the stage of 12 weeks and the model lasts for 8 weeks (scale bar, 1 mm). M , N Morphological parameters of trabecular bone in each group. n = 6 biologically independent mice per group. Data were showed as mean ± SD. Two-sided Student’s t test ( B , C , E , G , H , I ) was employed to assess the difference between the two groups. Two-way ANOVA ( M , N ) was utilized for multiple comparisons test. Significance: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, ns non-significance.

Article Snippet: For serums from patients, Pla2g7 (Cat#E-EL-H2286, Elabscience, China), CTX (Cat#CSB-E11224h, CUSABIO, China) and PINP (Cat#CSB-E11226h, CUSABIO, China) were tested utilizing human ELISA assay kit and the relevance was analyzed using Pearson correlation coefficient.

Techniques: Staining, Labeling, Expressing, Enzyme-linked Immunosorbent Assay, Knock-Out

Journal: Arthritis research & therapy

Article Title: Effect of alendronate on post-traumatic osteoarthritis induced by anterior cruciate ligament rupture in mice.

doi: 10.1186/s13075-015-0546-0

Figure Lengend Snippet: Figure 5 Serum biomarkers of bone resorption, C-terminal telopeptide of type I collagen (CTX-I) and of bone formation, procollagen type 1 N-terminal propeptide (P1NP) indicated that structural bone changes observed following injury are due to increased bone resorption with no change in bone formation. CTX-I exhibited the greatest increase in concentration at 14 days post-injury. This increase was effectively reduced by alendronate treatment, but remained higher in injured animals than uninjured animals. No significant differences in P1NP were observed for any experimental groups at any time points. VEH, vehicle; ALN-H, high-dose alendronate; ALN-L, low-dose alendronate.

Article Snippet: Blood serum was analyzed for cross-linked C-terminal telopeptide of type I collagen (CTX-I) and procollagen type 1 N-terminal propeptide (P1NP) (CUSABio ELISA kit, Wuhan Huamai Biotech Co., Wuhan, China).

Techniques: Concentration Assay

Journal: Nature Communications

Article Title: CUG-binding protein 1 regulates HSC activation and liver fibrogenesis

doi: 10.1038/ncomms13498

Figure Lengend Snippet: ( a ) Quantitative PCR analyses of CUGBP1 from LX-2 cells transfected with si-CUGBP1 or control siRNA for 48 h. ( b , c ) After transfection with si-CUGBP1 or control siRNA for 48 h, LX-2 cells were treated with or without 5 ng ml −1 TGF-β for 24 h. Cell extracts were subjected to quantitative PCR analyses (mean±s.e.m.; n =3) ( b ) and Western blot analysis ( c ). ( d ) ELISA measurement of supernatant IFN-γ (mean±s.e.m.; n =3). ( e ) Immunofluorescence analysis of CUGBP1, α-SMA and IFN-γ from primary mouse HSCs that were transfected with si-CUGBP1 or control siRNA for 48 h and then treated with or without 5 ng ml −1 TGF-β for 24 h (Scale bars, 50 μm). The data are representative of three independent experiments. ** P <0.01, *** P <0.001 by Student's t -test.

Article Snippet: ELISA kits for mouse procollagen III N-terminal peptide (CSB-E07928M) and hyaluronic acid (CSB-E08121M) were bought from Cosmo Bio Co., Ltd., (Tokyo, Japan).

Techniques: Real-time Polymerase Chain Reaction, Transfection, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Nature Communications

Article Title: CUG-binding protein 1 regulates HSC activation and liver fibrogenesis

doi: 10.1038/ncomms13498

Figure Lengend Snippet: BDL was performed in 8-week-old C57BL/6 mice by tying the common bile duct using a nonabsorbable filament. Fraxinellone was administrated by gavage once a day for 4 weeks after BDL operation. Mice were randomly divided into 5 groups ( n =8 in every group): Sham, BDL, BDL+fraxinellone 10 mg kg −1 , BDL+fraxinellone 20 mg kg −1 and BDL+fraxinellone 40 mg kg −1 . ( a ) Representative photograph of liver tissues, and microphotograph of H&E-stained, Masson-stained and Sirius Red-stained paraffin-embedded sections of liver tissues (Scale bars, 100 μm). ( b ) Collagen score of the Masson-stained sections. ( c ) Expression level of liver Hyp. ( d ) ELISA analysis of serum hyaluronic acid, laminin, and type III procollagen. ( e ) Quantitative PCR analysis of α-SMA, collagen α1(III), and collagen α1(IV) from mouse liver. ( b – e , mean±s.e.m.; n =8). * P <0.05, ** P <0.01, and *** P <0.001 versus BDL by one-way analysis of variance followed by Dunnett's test, # P <0.05, and ### P <0.001 versus sham by Student's t -test.

Article Snippet: ELISA kits for mouse procollagen III N-terminal peptide (CSB-E07928M) and hyaluronic acid (CSB-E08121M) were bought from Cosmo Bio Co., Ltd., (Tokyo, Japan).

Techniques: Staining, Expressing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction