procaspase-9 Search Results


33 murine procaspase 9  (Enzo Biochem)
90
Enzo Biochem 33 murine procaspase 9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
33 Murine Procaspase 9, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase-9  (SMAC Corp)
90
SMAC Corp procaspase-9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Procaspase 9, supplied by SMAC Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti–procaspase-9 antibody  (Becton Dickinson)
90
Becton Dickinson rabbit anti–procaspase-9 antibody
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Rabbit Anti–Procaspase 9 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase-9 caspase-9  (Stressgen Biotechnologies)
90
Stressgen Biotechnologies procaspase-9 caspase-9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Procaspase 9 Caspase 9, supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase-9  (Stressgen Biotechnologies)
90
Stressgen Biotechnologies procaspase-9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Procaspase 9, supplied by Stressgen Biotechnologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody rabbit anti-procaspase 9  (GeneTex)
90
GeneTex antibody rabbit anti-procaspase 9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Antibody Rabbit Anti Procaspase 9, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal human anti-procaspase 9  (Becton Dickinson)
90
Becton Dickinson monoclonal human anti-procaspase 9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Monoclonal Human Anti Procaspase 9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase-9  (Becton Dickinson)
90
Becton Dickinson procaspase-9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Procaspase 9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-procaspase-9 antibody (cat. no. adi-aam-139)  (Enzo Biochem)
90
Enzo Biochem anti-procaspase-9 antibody (cat. no. adi-aam-139)
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Anti Procaspase 9 Antibody (Cat. No. Adi Aam 139), supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase-9  (MBL Life science)
90
MBL Life science procaspase-9
Cyt C-mediates <t>procaspase</t> <t>9</t> persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.
Procaspase 9, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase-9 (cat. no. 66169-1-ig)  (Wuhan Sanying Biotechnology)
90
Wuhan Sanying Biotechnology procaspase-9 (cat. no. 66169-1-ig)
Effects of pseudo-G-Rh2 on caspase activity in A549 cells. A549 cells were incubated with 24, 48 or 96 µM pseudo-G-Rh2 for 24 h. (A) The expression of caspases-9, caspase-3 and PARP was determined using western blotting. (B) Caspase-9 and caspase-3 activities were assessed using Ac-LEHD-pNA and Ac-DEVD-pNA, respectively. Western blotting results for (C) procaspase-3, (D) <t>procaspase-9</t> and (E) cleaved PARP were quantified. *P<0.05 vs. control. G, ginsenoside; PARP, poly ADP-ribose polymerase.
Procaspase 9 (Cat. No. 66169 1 Ig), supplied by Wuhan Sanying Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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procaspase 9 a full-length caspn9 cdna  (Abbott Laboratories)
90
Abbott Laboratories procaspase 9 a full-length caspn9 cdna
Effects of pseudo-G-Rh2 on caspase activity in A549 cells. A549 cells were incubated with 24, 48 or 96 µM pseudo-G-Rh2 for 24 h. (A) The expression of caspases-9, caspase-3 and PARP was determined using western blotting. (B) Caspase-9 and caspase-3 activities were assessed using Ac-LEHD-pNA and Ac-DEVD-pNA, respectively. Western blotting results for (C) procaspase-3, (D) <t>procaspase-9</t> and (E) cleaved PARP were quantified. *P<0.05 vs. control. G, ginsenoside; PARP, poly ADP-ribose polymerase.
Procaspase 9 A Full Length Caspn9 Cdna, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cyt C-mediates procaspase 9 persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.

Journal: ACS chemical biology

Article Title: Cytochrome c Reduction by H 2 S Potentiates Sulfide Signaling

doi: 10.1021/acschembio.8b00463

Figure Lengend Snippet: Cyt C-mediates procaspase 9 persulfdation and activity. (A) Murine recombinant procaspase 9 (1 μM) was incubated with 5 μM H2S for 30 min at 37 °C in the absence (lane 1) or presence of 0.5 (lane 2) and 2 μM (lane 3) Cyt C. Persulfidation was detected by the CN-Cy3-based tag switch method (top panel), while equal loading was confirmed by Coomassie blue staining (bottom panel). (B) H2S inhibits Cyt C-induced caspase 9 activation in cell lysates. Cyt C (500 nM) was added to freshly prepared HeLa cell lysates, and caspase 9 activity was monitored using a fluorescence caspase 9 kit. Simultaneous addition of Cyt C and H2S (10 μM) inhibited the Cyt C-induced increase in fluorescence. (C) Cyt C-induced caspase 9 activation in Jurkat cells is inhibited by H2S donors. Apoptosis was induced by staurosporine (ST, 2.5 μM), and caspase 9 activity was monitored by flow cytometry using the caspase 9 FITC staining kit 4 and 6 h after induction. Treatment of cells with GYY4137 (100 μM) or ammonium tetrathiomolybdate (ATTM, 200 μM) reduced fluorescence. (D) Inhibition of caspase 3 activation in cells treated with antimycin A and the slow-releasing H2S donor, GYY4137 (100 μM). HeLa cells were incubated with antimycin A (300 μM) overnight to induce the release of Cyt C from mitochondria. GYY4137, when used, was added 2 h prior to antimycin A. Caspase 3 activity was monitored in cell lysates as described for panel B. (E) Persulfidation of procaspase 9 in HeLa cells treated with 2.5 μM ST (1) that were either pretreated for 2 h (2) or treated for the last 2 h (3) with GYY4137 (100 μM): (left) total procaspase 9 levels and (right) persulfidation (PSSH) levels of procaspase 9. PSSH levels were quantified by measuring the difference in fluorescence between DTT-treated and untreated samples and normalizing this to the total procaspase 9 levels. The strategy used for sample processing to detect total versus persulfidated procaspase 9 is explained in the Supporting Information and Supporting Figure 5.

Article Snippet: 33 Murine procaspase 9 was from Enzo Life Science.

Techniques: Activity Assay, Recombinant, Incubation, Staining, Activation Assay, Fluorescence, Flow Cytometry, Inhibition

Effects of pseudo-G-Rh2 on caspase activity in A549 cells. A549 cells were incubated with 24, 48 or 96 µM pseudo-G-Rh2 for 24 h. (A) The expression of caspases-9, caspase-3 and PARP was determined using western blotting. (B) Caspase-9 and caspase-3 activities were assessed using Ac-LEHD-pNA and Ac-DEVD-pNA, respectively. Western blotting results for (C) procaspase-3, (D) procaspase-9 and (E) cleaved PARP were quantified. *P<0.05 vs. control. G, ginsenoside; PARP, poly ADP-ribose polymerase.

Journal: Experimental and Therapeutic Medicine

Article Title: Pseudo-Ginsenoside Rh2 induces A549 cells apoptosis via the Ras/Raf/ERK/p53 pathway

doi: 10.3892/etm.2018.6067

Figure Lengend Snippet: Effects of pseudo-G-Rh2 on caspase activity in A549 cells. A549 cells were incubated with 24, 48 or 96 µM pseudo-G-Rh2 for 24 h. (A) The expression of caspases-9, caspase-3 and PARP was determined using western blotting. (B) Caspase-9 and caspase-3 activities were assessed using Ac-LEHD-pNA and Ac-DEVD-pNA, respectively. Western blotting results for (C) procaspase-3, (D) procaspase-9 and (E) cleaved PARP were quantified. *P<0.05 vs. control. G, ginsenoside; PARP, poly ADP-ribose polymerase.

Article Snippet: Antibodies against procaspase-3 (cat. no. 19677-1-AP), procaspase-9 (cat. no. 66169-1-Ig) and β-actin (cat. no. 20536-1-AP) were purchased from Wuhan Sanying Biotechnology (Wuhan, China).

Techniques: Activity Assay, Incubation, Expressing, Western Blot