Journal: PLoS ONE

Article Title: Effect of Change in Spindle Structure on Proliferation Inhibition of Osteosarcoma Cells and Osteoblast under Simulated Microgravity during Incubation in Rotating Bioreactor

doi: 10.1371/journal.pone.0076710

Figure Lengend Snippet: Cell-cycle histograms of MG-63(A), U-2 OS (B) cells and rat calvaria primary osteoblast(C) under simulated microgravity for 72 hours. The cycle changes of MG-63(D), U-2 OS(E), hFOB1.19(F) cells and rat calvaria primary osteoblast(G) under simulated microgravity. For MG-63 and U-2 OS the cell number in G1 phase decreases and increases in S and G2/M phase under simulated microgravity. For hFOB1.19 cells, increase in S phase under simulated microgravity. Rat calvaria primary osteoblasts increases only in G2 phases under simulated microgravity for 72 hours. The data shown represent the mean± S.E. (n = 3). *0.01< P <0.05, and ** P <0.01 ( t -test as compared to untreated control group).

Article Snippet: And primary osteoblasts derived from fetal Sprague-Dawley rat calvaria are cultured in DMEM (Gibco-BRL) with 10% (v/v) FBS (Gibco-BRL) .

Techniques: Control

Journal: PLoS ONE

Article Title: Effect of Change in Spindle Structure on Proliferation Inhibition of Osteosarcoma Cells and Osteoblast under Simulated Microgravity during Incubation in Rotating Bioreactor

doi: 10.1371/journal.pone.0076710

Figure Lengend Snippet: In hFOB1.19 cells (A and B), early apoptosis and late death mean extremely significant differences between untreated control and simulated microgravity for 24–72 hours. In rat calvaria primary osteoblasts (C and D), early apoptosis and late death mean significant differences between untreated control and simulated microgravity for 72 hours.The data shown represent the mean± S.E. (n = 3). *0.01< P <0.05, and ** P <0.01 ( t -test as compared to untreated control group).

Article Snippet: And primary osteoblasts derived from fetal Sprague-Dawley rat calvaria are cultured in DMEM (Gibco-BRL) with 10% (v/v) FBS (Gibco-BRL) .

Techniques: Control

Journal: PLoS ONE

Article Title: Effect of Change in Spindle Structure on Proliferation Inhibition of Osteosarcoma Cells and Osteoblast under Simulated Microgravity during Incubation in Rotating Bioreactor

doi: 10.1371/journal.pone.0076710

Figure Lengend Snippet: The spindles are stained green by anti-α-tubulin first antibody and FITC-conjugated goat anti-mouse IgG. A and B. Bipolar spindle of MG-63(A) cell and rat calvaria primary osteoblast (B) under 1 g condition. C–E. Multipolar spindle under simulated microgravity in MG-63 cell (C. three polar spindles, D. four polar spindles) and hFOB1.19 cell (E. five polar spindles). F–H. Microtubule arrangement change under simulated microgravity (F. MG-63, G. U-2 OS, H. rat calvaria primary osteoblast).

Article Snippet: And primary osteoblasts derived from fetal Sprague-Dawley rat calvaria are cultured in DMEM (Gibco-BRL) with 10% (v/v) FBS (Gibco-BRL) .

Techniques: Staining

Journal: PLoS ONE

Article Title: Effect of Change in Spindle Structure on Proliferation Inhibition of Osteosarcoma Cells and Osteoblast under Simulated Microgravity during Incubation in Rotating Bioreactor

doi: 10.1371/journal.pone.0076710

Figure Lengend Snippet: The results demonstrate significant increase in multipolar spindle incidence along with simulated microgravity duration, and exhibit significant difference resulting from being cultured for 72 hours(MG-63, A), 48 hours(U-2 OS(B) and hFOB1.19(C)), or even 24 hours(rat calvaria primary osteoblast, D). The data shown represent the mean±S.E. (n = 3). *0.01< P <0.05, ** P <0.01, and *** P <0.001 ( -test as compared to rotation control group).

Article Snippet: And primary osteoblasts derived from fetal Sprague-Dawley rat calvaria are cultured in DMEM (Gibco-BRL) with 10% (v/v) FBS (Gibco-BRL) .

Techniques: Cell Culture, Control