Journal: International Journal of Molecular Medicine

Article Title: miR-181b-p53 negative feedback axis regulates osteosarcoma cell proliferation and invasion

doi: 10.3892/ijmm.2020.4558

Figure Lengend Snippet: miR-181b and p53 expression in OS tissues and cell lines The expression of (A) miR-181b and (B) p53 in noncancerous and OS tissue samples was determined by qPCR. (C) The correlation between miR-181b and p53 expression in OS tissue samples. The expression of (D) miR-181b and (E) p53 in normal HOBs and two OS cell lines U2OS and MG63 cells, was determined by qPCR. ** P<0.01. miR, microRNA; OS, osteosarcoma; NC, negative control; q, quantitative; HOBs, human osteoblasts.

Article Snippet: Primary human osteoblasts (HOBs) were obtained from Merck KGaA (cat. no. 406-05F) and cultured in a 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium (both from Gibco; Thermo Fisher Scientific, Inc.), with 2.5 mM L-glutamine (without phenol red) and 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Negative Control

Journal: Bone

Article Title: Ex Vivo Construction of Human Primary 3D-Networked Osteocytes

doi: 10.1016/j.bone.2017.09.012

Figure Lengend Snippet: [32] (a) microfluidic perfusion device with 6 culture chambers, (b) cross-sectional view of a 3D culture chamber with the red arrows indicating the overall direction of culture medium flow through the device, (c) schematic illustration of microbeads-guided assembly, and (d) histologic image of 3D-networked osteocytes with the red arrows indicating medium flow direction with respect to the tissue sample. Scale bar: 20 µm.

Article Snippet: A human 3D bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via: (1) the biomimetic assembly of primary human osteoblastic cells with 20–25 μm and BCP microbeads and (2) subsequent microfluidic perfusion culture.

Techniques:

Journal: Bone

Article Title: Ex Vivo Construction of Human Primary 3D-Networked Osteocytes

doi: 10.1016/j.bone.2017.09.012

Figure Lengend Snippet: (a) hip fragment shown as an example; (b) as-isolated cells after 4 collagenase digestion cycles; (c) proliferated osteoblastic cells after 10 days of 2D culture; (d) 3D tissue sample constructed using 20–25 µm microbeads and proliferated cells and 14 days of perfusion culture; (e) H&E histologic images showing the formation of 3D cellular network as indicated by black arrows in (f) and white arrows in (g); and (h) immunostaining for sclerostin (red). (d) –(f) from patient sample #6 and (g)–(h) from patient sample #4. Scale bar: 25 µm.

Article Snippet: A human 3D bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via: (1) the biomimetic assembly of primary human osteoblastic cells with 20–25 μm and BCP microbeads and (2) subsequent microfluidic perfusion culture.

Techniques: Isolation, Construct, Immunostaining

Journal: Nanomaterials

Article Title: Synthesis of Gold Nanoparticles by Using Green Machinery: Characterization and In Vitro Toxicity

doi: 10.3390/nano11030808

Figure Lengend Snippet: Assessment of GNPs cytotoxicity and determination GNPs IC 50 on cancer cells. Normal human primary osteoblasts and human cervical cancer cell (HeLa cells) were plated overnight at a density of 1 × 10 4 cell per well in a 96-well plate at 37 °C. The normal or cancer cells were treated with different concentrations of GNPs and the in vitro cytotoxicity was evaluated using MTT assay. The inhibition percentages were calculated relative to negative control and IC 50 was the GNPs concentration, which inhibits 50% of HeLa cells. The experiment was conducted in triplicate and the data shown are the means ± standard errors.

Article Snippet: The normal human primary osteoblasts and human cervical cancer cells (HeLa) were supplied from National Centre for Cell Science (NCCS), Pune, India.

Techniques: In Vitro, MTT Assay, Inhibition, Negative Control, Concentration Assay