Journal: Pharmaceuticals

Article Title: Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations

doi: 10.3390/ph15091105

Figure Lengend Snippet: Illustration of Round Window Membrane Cross Section . The outer layer of the RWM consists of stratified columnar epithelial cells. The middle layer consists of fibroblasts embedded in connective tissue (primarily collagen and elastin). The inner layer of the RWM is made up of thin squamous epithelial cells. This figure was drawn using modified artwork from Servier Medical Art. Servier Medical Art by Servier is licensed under a Creative Commons Attribution 3.0 Unported License ( https://creativecommons.org/licenses/by/3.0/ ) (accessed on 10 July 2021).

Article Snippet: Primary human small airway epithelial cells (HSAEC; normal (ATCC PCS301010, Manassas, VA, USA) were cultured in airway epithelial cell basal media supplemented with bronchial epithelial cell growth kit (ATCC PCS300040, Manassas, VA, USA) components.

Techniques: Membrane, Modification

Journal: Pharmaceuticals

Article Title: Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations

doi: 10.3390/ph15091105

Figure Lengend Snippet: Tissue barrier properties of AET constructs. Constructs at day 14 and 21, respectively, in culture, as evaluated by transepithelial electrical resistance (TEER) measurements. Unpaired T test, * p < 0.05, n = 5. AET, airway epithelial tissues.

Article Snippet: Primary human small airway epithelial cells (HSAEC; normal (ATCC PCS301010, Manassas, VA, USA) were cultured in airway epithelial cell basal media supplemented with bronchial epithelial cell growth kit (ATCC PCS300040, Manassas, VA, USA) components.

Techniques: Construct

Journal: Pharmaceuticals

Article Title: Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations

doi: 10.3390/ph15091105

Figure Lengend Snippet: Drug permeation in day 14 AET constructs . ( a ) Fluorescein, ( b ) DSP, ( c ) ciprofloxacin HCl, ( d ) gentamicin sulfate. n = 4 for all drugs; lines show mean. AET, airway epithelial tissues; DSP, dexamethasone sodium phosphate.

Article Snippet: Primary human small airway epithelial cells (HSAEC; normal (ATCC PCS301010, Manassas, VA, USA) were cultured in airway epithelial cell basal media supplemented with bronchial epithelial cell growth kit (ATCC PCS300040, Manassas, VA, USA) components.

Techniques: Construct

Journal: Pharmaceuticals

Article Title: Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations

doi: 10.3390/ph15091105

Figure Lengend Snippet: Drug permeation in day 21 AET constructs . ( a ) Fluorescein, ( b ) DSP, ( c ) ciprofloxacin HCl, ( d ) gentamicin sulfate. n = 4 for all drugs; lines show mean. AET, airway epithelial tissues; DSP, dexamethasone sodium phosphate.

Article Snippet: Primary human small airway epithelial cells (HSAEC; normal (ATCC PCS301010, Manassas, VA, USA) were cultured in airway epithelial cell basal media supplemented with bronchial epithelial cell growth kit (ATCC PCS300040, Manassas, VA, USA) components.

Techniques: Construct

Journal: Pharmaceuticals

Article Title: Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations

doi: 10.3390/ph15091105

Figure Lengend Snippet: Histological and immunofluorescent evaluation of AET constructs subjected to freeze/thaw cycles . H&E (left), nuclear (DAPI, blue), claudin-1 (CLDN-1, green), and zona occludens-1 (ZO-1, red) staining of unprocessed (top row), freeze/thawed once (middle row), or freeze/thawed twice (bottom row). Scale bar 50 μm for H&E; scale bar 20 μm for immunofluorescence; n = 4. AET, airway epithelial tissue; CLDN-1, claudin-1; DAPI, 4′,6-diamidino-2-phenylindole; H&E, hematoxylin and eosin; ZO-1, zona occludens-1.

Article Snippet: Primary human small airway epithelial cells (HSAEC; normal (ATCC PCS301010, Manassas, VA, USA) were cultured in airway epithelial cell basal media supplemented with bronchial epithelial cell growth kit (ATCC PCS300040, Manassas, VA, USA) components.

Techniques: Construct, Staining, Immunofluorescence

Journal: Pharmaceuticals

Article Title: Development and Characterization of an In Vitro Round Window Membrane Model for Drug Permeability Evaluations

doi: 10.3390/ph15091105

Figure Lengend Snippet: Drug permeation in day 14 AET constructs subjected to freeze/thaw cycles . ( a ) Fluorescein, ( b ) DSP, ( c ) ciprofloxacin HCl, ( d ) gentamicin sulfate. n = 4 for all drugs; lines show mean. AET, airway epithelial tissue; DSP, dexamethasone sodium phosphate.

Article Snippet: Primary human small airway epithelial cells (HSAEC; normal (ATCC PCS301010, Manassas, VA, USA) were cultured in airway epithelial cell basal media supplemented with bronchial epithelial cell growth kit (ATCC PCS300040, Manassas, VA, USA) components.

Techniques: Construct

Journal: Antioxidants (Basel, Switzerland)

Article Title: Anti-Inflammatory Effects of Olive Leaf Extract and Its Bioactive Compounds Oleacin and Oleuropein-Aglycone on Senescent Endothelial and Small Airway Epithelial Cells.

doi: 10.3390/antiox12081509

Figure Lengend Snippet: Figure 6. OLE, OC and OA anti-inflammatory and anti-viral effects on hSAEC cells. Cells were pre-treated with OLE (4 µg/mL), OC (1 µM) and OA (5 µM) for 2 h and then stimulated with Poly(I:C) for 24 h. (A) NF-kB, IL-1β, IL-6, TNFα, IL-8, ICAM-1, SOD2, ACE2 and TMPRSS2 mRNA expression. Data are shown as fold change vs. untreated Poly(I:C)-stimulated hSAEC according to 2-∆∆Ct method, using actin as housekeeping. (B) IL-6 (pg/mL) in the culture medium. (C) Representative Western blot analysis showing P-NF-κB, ACE2 and TMPRSS2 protein expression, with β-actin as loading control. The results are expressed as mean ± SD from three independent biological replicates. Paired t test, # p < 0.05, ## p < 0.01, ### p < 0.001 vs. untreated hSAEC (CTR); * p < 0.05, ** p < 0.01 vs. Poly(I:C)-stimulated hSAEC (POLY).

Article Snippet: Primary human small airway epithelial cells (hSAEC) were purchased from ATCC (PCS-301-010) and grown to confluence in a humidified atmosphere at 37 ◦C and 5% CO2, seeded at a density of 5000/cm2 with Airway Epithelial Cell Basal Medium (ATCC.

Techniques: Expressing, Western Blot, Control