primary cardiac fibroblasts Search Results


mouse cardiac fibroblasts primary cells complete growth medium  (Innovative Research Inc)
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Mouse Cardiac Fibroblasts Primary Cells Complete Growth Medium is a primary cells complete growth medium from Innovative Research, supplied as a ready-to-use liquid. More Details: Bacterial detection: Negative Fungal detection: Negative Mycoplasma detection: Negative Endotoxin
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human adult primary ventricular cardiac fibroblasts avhcf lot 9602  (ScienCell)
90
ScienCell human adult primary ventricular cardiac fibroblasts avhcf lot 9602
Human Adult Primary Ventricular Cardiac Fibroblasts Avhcf Lot 9602, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+cardiac+fibroblasts/human+adult+primary+ventricular+cardiac+fibroblasts+avhcf+lot+9602/pmc11422419-24-0-12
Average 90 stars, based on 1 article reviews
human adult primary ventricular cardiac fibroblasts avhcf lot 9602 - by Bioz Stars, 2026-09
90/100 stars
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rat primary cardiac fibroblasts  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc rat primary cardiac fibroblasts
Effect of ang II on the expression of TIMP1 , TGF-β/Smad pathway-related proteins, and α-SMA. (A) qRT-PCR was performed to assess the mRNA expression levels of TIMP1 in rat cardiac <t>fibroblasts</t> treated with different concentrations of ang II (1 nM, 10 nM, 100 nM, and 1 μM). (B) qRT-PCR was also performed to assess the mRNA expression levels of TIMP1 in rat atrial fibroblasts subjected to different durations of 1 μM of ang II treatment (12, 24, and 48 hours). (C) WB was used to detect the expression of TGF-β1, p-Smad2, p-Smad3, Smad2, Smad3 and α-SMA after 48 hours of treatment with 1 μM of ang II. (D) The results of WB analysis for the same proteins are shown. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.
Rat Primary Cardiac Fibroblasts, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+cardiac+fibroblasts/rat+primary+cardiac+fibroblasts/pmc12268815-81-0-7
Average 90 stars, based on 1 article reviews
rat primary cardiac fibroblasts - by Bioz Stars, 2026-09
90/100 stars
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primary rat cardiac fibroblasts (rcfs)  (Japan SLC inc)
90
Japan SLC inc primary rat cardiac fibroblasts (rcfs)
Effect of ang II on the expression of TIMP1 , TGF-β/Smad pathway-related proteins, and α-SMA. (A) qRT-PCR was performed to assess the mRNA expression levels of TIMP1 in rat cardiac <t>fibroblasts</t> treated with different concentrations of ang II (1 nM, 10 nM, 100 nM, and 1 μM). (B) qRT-PCR was also performed to assess the mRNA expression levels of TIMP1 in rat atrial fibroblasts subjected to different durations of 1 μM of ang II treatment (12, 24, and 48 hours). (C) WB was used to detect the expression of TGF-β1, p-Smad2, p-Smad3, Smad2, Smad3 and α-SMA after 48 hours of treatment with 1 μM of ang II. (D) The results of WB analysis for the same proteins are shown. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.
Primary Rat Cardiac Fibroblasts (Rcfs), supplied by Japan SLC inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+cardiac+fibroblasts/primary+rat+cardiac+fibroblasts++rcfs+/pm31229551-46-0-19
Average 90 stars, based on 1 article reviews
primary rat cardiac fibroblasts (rcfs) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
mouse primary cardiac fibroblasts  (Pro-cell Co Ltd)
90
Pro-cell Co Ltd mouse primary cardiac fibroblasts
Effect of ang II on the expression of TIMP1 , TGF-β/Smad pathway-related proteins, and α-SMA. (A) qRT-PCR was performed to assess the mRNA expression levels of TIMP1 in rat cardiac <t>fibroblasts</t> treated with different concentrations of ang II (1 nM, 10 nM, 100 nM, and 1 μM). (B) qRT-PCR was also performed to assess the mRNA expression levels of TIMP1 in rat atrial fibroblasts subjected to different durations of 1 μM of ang II treatment (12, 24, and 48 hours). (C) WB was used to detect the expression of TGF-β1, p-Smad2, p-Smad3, Smad2, Smad3 and α-SMA after 48 hours of treatment with 1 μM of ang II. (D) The results of WB analysis for the same proteins are shown. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.
Mouse Primary Cardiac Fibroblasts, supplied by Pro-cell Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+cardiac+fibroblasts/mouse+primary+cardiac+fibroblasts/pm37610394-51-0-7
Average 90 stars, based on 1 article reviews
mouse primary cardiac fibroblasts - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rat cardiac fibroblasts primary cells complete growth medium  (Innovative Research Inc)
N/A
Rat Cardiac Fibroblasts Primary Cells Complete Growth Medium is a primary cells complete growth medium from Innovative Research, supplied as a ready-to-use liquid. More Details: Bacterial detection: Negative Fungal detection: Negative Mycoplasma detection: Negative Endotoxin
   Buy from Supplier

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Effect of ang II on the expression of TIMP1 , TGF-β/Smad pathway-related proteins, and α-SMA. (A) qRT-PCR was performed to assess the mRNA expression levels of TIMP1 in rat cardiac fibroblasts treated with different concentrations of ang II (1 nM, 10 nM, 100 nM, and 1 μM). (B) qRT-PCR was also performed to assess the mRNA expression levels of TIMP1 in rat atrial fibroblasts subjected to different durations of 1 μM of ang II treatment (12, 24, and 48 hours). (C) WB was used to detect the expression of TGF-β1, p-Smad2, p-Smad3, Smad2, Smad3 and α-SMA after 48 hours of treatment with 1 μM of ang II. (D) The results of WB analysis for the same proteins are shown. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.

Journal: Journal of Thoracic Disease

Article Title: TIMP1 regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an in vitro model of atrial fibrillation

doi: 10.21037/jtd-2025-1088

Figure Lengend Snippet: Effect of ang II on the expression of TIMP1 , TGF-β/Smad pathway-related proteins, and α-SMA. (A) qRT-PCR was performed to assess the mRNA expression levels of TIMP1 in rat cardiac fibroblasts treated with different concentrations of ang II (1 nM, 10 nM, 100 nM, and 1 μM). (B) qRT-PCR was also performed to assess the mRNA expression levels of TIMP1 in rat atrial fibroblasts subjected to different durations of 1 μM of ang II treatment (12, 24, and 48 hours). (C) WB was used to detect the expression of TGF-β1, p-Smad2, p-Smad3, Smad2, Smad3 and α-SMA after 48 hours of treatment with 1 μM of ang II. (D) The results of WB analysis for the same proteins are shown. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; mRNA, messenger RNA; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.

Article Snippet: Rat primary cardiac fibroblasts were purchased from Stemcell Biotechnology Co., Ltd. (cat. no. STM-CE-3303; Shanghai, China; https://www.stemrecell.com/primary-cell-rat-fibroblast/cardiacmuscle.html ).

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

TIMP1 modulation altered the ang II-mediated effects on rat atrial fibroblast viability and proliferation. (A,B) qRT-PCR and WB were used to detect the TIMP1 knockdown efficiency in rat cardiac fibroblasts treated with 1 μM of ang I for 48 hours. (C,D) Evaluation of TIMP1 overexpression efficiency in rat atrial fibroblasts after 48 hours of 1-μM ang II treatment. (E) qRT-PCR was used to detect the expression of TIMP1 in cardiac fibroblasts treated with 1 μM of ang II for 48 hours following knockdown or overexpression of TIMP1 . (F) Cell viability of rat cardiac fibroblasts was assessed by CCK-8 assay after treatment with 1 μM of ang II, either alone or in combination with negative control (NC), TIMP1 knockdown (si- TIMP1 -1), or TIMP1 overexpression (over- TIMP1 ). (G) Colony formation assay results of rat cardiac fibroblasts treated with control, 1 μM of ang II alone, ang II combined with NC, si- TIMP1 -1, or over- TIMP1 . Observed using a stereomicroscope and photographed with a scanner. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; CCK-8, Cell Counting Kit-8; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.

Journal: Journal of Thoracic Disease

Article Title: TIMP1 regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an in vitro model of atrial fibrillation

doi: 10.21037/jtd-2025-1088

Figure Lengend Snippet: TIMP1 modulation altered the ang II-mediated effects on rat atrial fibroblast viability and proliferation. (A,B) qRT-PCR and WB were used to detect the TIMP1 knockdown efficiency in rat cardiac fibroblasts treated with 1 μM of ang I for 48 hours. (C,D) Evaluation of TIMP1 overexpression efficiency in rat atrial fibroblasts after 48 hours of 1-μM ang II treatment. (E) qRT-PCR was used to detect the expression of TIMP1 in cardiac fibroblasts treated with 1 μM of ang II for 48 hours following knockdown or overexpression of TIMP1 . (F) Cell viability of rat cardiac fibroblasts was assessed by CCK-8 assay after treatment with 1 μM of ang II, either alone or in combination with negative control (NC), TIMP1 knockdown (si- TIMP1 -1), or TIMP1 overexpression (over- TIMP1 ). (G) Colony formation assay results of rat cardiac fibroblasts treated with control, 1 μM of ang II alone, ang II combined with NC, si- TIMP1 -1, or over- TIMP1 . Observed using a stereomicroscope and photographed with a scanner. Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; CCK-8, Cell Counting Kit-8; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.

Article Snippet: Rat primary cardiac fibroblasts were purchased from Stemcell Biotechnology Co., Ltd. (cat. no. STM-CE-3303; Shanghai, China; https://www.stemrecell.com/primary-cell-rat-fibroblast/cardiacmuscle.html ).

Techniques: Quantitative RT-PCR, Knockdown, Over Expression, Expressing, CCK-8 Assay, Negative Control, Colony Assay, Control, Cell Counting, Real-time Polymerase Chain Reaction, Western Blot

Effects of TIMP1 regulation on fibrosis marker expression and TGF-β/Smad signaling in ang II-induced rat cardiac fibroblasts. (A-C) The mRNA expression levels of fibrotic markers, collagen I , collagen III , and α-SMA in rat cardiac fibroblasts were analyzed via qRT-PCR and WB assay. The experimental groups of rat cardiac fibroblasts were as follows: control, 1 μM of ang II, 1 μM of ang II + NC, 1 μM of ang II + si- TIMP1- 1, and 1 μM of ang II + over- TIMP1 . (D,E) WB analysis of the protein expression levels of the TGF-β/Smad3 signaling pathway components in rat cardiac fibroblasts, including TGF-β1, phosphorylated Smad2 (p-Smad2), phosphorylated Smad3 (p-Smad3), Smad2, and Smad3. The experimental groups of rat cardiac fibroblasts were as follows: control, 1 μM of ang II, 1 μM of ang II + NC, 1 μM of ang II + si- TIMP1- 1, and 1 μM of ang II + over- TIMP1 . Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; NC, negative control; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.

Journal: Journal of Thoracic Disease

Article Title: TIMP1 regulation of cardiac fibroblast proliferation via the TGF-β/Smad pathway in an in vitro model of atrial fibrillation

doi: 10.21037/jtd-2025-1088

Figure Lengend Snippet: Effects of TIMP1 regulation on fibrosis marker expression and TGF-β/Smad signaling in ang II-induced rat cardiac fibroblasts. (A-C) The mRNA expression levels of fibrotic markers, collagen I , collagen III , and α-SMA in rat cardiac fibroblasts were analyzed via qRT-PCR and WB assay. The experimental groups of rat cardiac fibroblasts were as follows: control, 1 μM of ang II, 1 μM of ang II + NC, 1 μM of ang II + si- TIMP1- 1, and 1 μM of ang II + over- TIMP1 . (D,E) WB analysis of the protein expression levels of the TGF-β/Smad3 signaling pathway components in rat cardiac fibroblasts, including TGF-β1, phosphorylated Smad2 (p-Smad2), phosphorylated Smad3 (p-Smad3), Smad2, and Smad3. The experimental groups of rat cardiac fibroblasts were as follows: control, 1 μM of ang II, 1 μM of ang II + NC, 1 μM of ang II + si- TIMP1- 1, and 1 μM of ang II + over- TIMP1 . Each experiment was performed with at least three biological replicates. *, P<0.05. TIMP1 , tissue inhibitor of metalloproteinases-1; ang II, angiotensin II; NC, negative control; qRT-PCR, quantitative real-time polymerase chain reaction; WB, Western blotting.

Article Snippet: Rat primary cardiac fibroblasts were purchased from Stemcell Biotechnology Co., Ltd. (cat. no. STM-CE-3303; Shanghai, China; https://www.stemrecell.com/primary-cell-rat-fibroblast/cardiacmuscle.html ).

Techniques: Marker, Expressing, Quantitative RT-PCR, Control, Negative Control, Real-time Polymerase Chain Reaction, Western Blot