primary 184 antibody Search Results


alexa fluor 488 anti rabbit igg  (Jackson Immuno)
96
Jackson Immuno alexa fluor 488 anti rabbit igg
Alexa Fluor 488 Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/Alexa+Fluor+488+AffiniPure+Donkey+Anti-Rabbit+IgG/10__1523_slash_jneurosci__1922___20__2020-107-8-26
Average 96 stars, based on 1 article reviews
alexa fluor 488 anti rabbit igg - by Bioz Stars, 2026-09
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rabbit antihuman ap 2a polyclonal antibodies  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology rabbit antihuman ap 2a polyclonal antibodies
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Rabbit Antihuman Ap 2a Polyclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/Rab+2A+Antibody/pm12839558-61-8-16
Average 93 stars, based on 1 article reviews
rabbit antihuman ap 2a polyclonal antibodies - by Bioz Stars, 2026-09
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primary antibodies against phospho tak 1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc primary antibodies against phospho tak 1
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Primary Antibodies Against Phospho Tak 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/Phospho-TAK1+(Thr184)+Antibody/pmc03295657-41-0-26
Average 93 stars, based on 1 article reviews
primary antibodies against phospho tak 1 - by Bioz Stars, 2026-09
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rabbit anti myosin phosphatase targeting subunit 1 mypt1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti myosin phosphatase targeting subunit 1 mypt1
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Rabbit Anti Myosin Phosphatase Targeting Subunit 1 Mypt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/MYPT1+Antibody/ppr0229007-79-10-21
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rabbit anti myosin phosphatase targeting subunit 1 mypt1 - by Bioz Stars, 2026-09
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anti mtor  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc anti mtor
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Anti Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/mTOR+Rabbit+mAb/10__1177_slash_17448069251376198-103-8-11
Average 97 stars, based on 1 article reviews
anti mtor - by Bioz Stars, 2026-09
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anti wnt5a  (Proteintech)
96
Proteintech anti wnt5a
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Anti Wnt5a, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/WNT5A%2FB+Antibody/pmc10068476-96-31-34
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anti wnt5a - by Bioz Stars, 2026-09
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primary erα antibody  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology primary erα antibody
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Primary Erα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/ER%CE%B1/pmc02719673-176-32-37
Average 95 stars, based on 1 article reviews
primary erα antibody - by Bioz Stars, 2026-09
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primary 184 antibody  (Cedarlane)
93
Cedarlane primary 184 antibody
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Primary 184 Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/Anti-Salmonid+Ig+(H%2BL)%2C+Purified%2C+(Polyclonal)+(rabbit+IgG)/pm34252544-118-10-17
Average 93 stars, based on 1 article reviews
primary 184 antibody - by Bioz Stars, 2026-09
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3 3ack49 51  (St Johns Laboratory)
93
St Johns Laboratory 3 3ack49 51
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
3 3ack49 51, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/Anti-14-3-3++++antibody/ppr0123116-450-7-3
Average 93 stars, based on 1 article reviews
3 3ack49 51 - by Bioz Stars, 2026-09
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anti-brdu  (Becton Dickinson)
90
Becton Dickinson anti-brdu
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Anti Brdu, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/brdu+flow+kit/pm30896968-304-25-29
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anti-brdu - by Bioz Stars, 2026-09
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gremlin 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology gremlin 1
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
Gremlin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+184+antibody/gremlin-1+Antibody/pmc03693206-57-6-11
Average 93 stars, based on 1 article reviews
gremlin 1 - by Bioz Stars, 2026-09
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184 antibody  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 184 antibody
Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
184 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.

Journal: The Journal of investigative dermatology

Article Title: Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling.

doi: 10.1046/j.1523-1747.2003.12319.x

Figure Lengend Snippet: Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.

Article Snippet: After the overnight incubation (room temperature) with primary rabbit antihuman AP-2a polyclonal antibodies (sc-184, clone C-18; Santa Cruz Biotechnology, Santa Cruz, CA) at a dilution of 1:500, the sections were treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG (Jackson ImmunoResearch,West Grove, PA) at a dilution of 1:200 (1 h; 371C), counterstained with Hoechst 33342 dye (10 mg per ml) (Sigma, St. Louis, MO), and mounted with Vectashield H-1000 (Vector, Burlingame, CA).

Techniques: Expressing, Incubation