Journal: Current Biology

Article Title: Microtubule Minus-End Binding Protein CAMSAP2 and Kinesin-14 Motor KIFC3 Control Dendritic Microtubule Organization

doi: 10.1016/j.cub.2019.12.056

Figure Lengend Snippet:

Article Snippet: Streptavidin pull-down assays were performed by using Dynabeads M-280 streptavidin beads (Invitrogen).

Techniques: Recombinant, Plasmid Preparation, Software

Journal: Cell reports

Article Title: Single-cell sequencing reveals activation of core transcription factors in PRC2-deficient malignant peripheral nerve sheath tumor

doi: 10.1016/j.celrep.2022.111363

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies against FOXC1, EZH2, SUZ12 and IgG isotype control were incubated overnight with Dynabeads M-280 Sheep anti-Rabbit IgG magnetic beads (Invitrogen) followed with washing using Washing Buffer (50 mM Tris-HCl pH 7.5, 137 mM NaCl, 0.005 Triton X-100, 1 mM EDTA, de-ionized H 2 O).

Techniques: Control, Virus, Recombinant, Lysis, Protease Inhibitor, Western Blot, Saline, Bradford Assay, Extraction, Magnetic Beads, Multiplex Assay, Next-Generation Sequencing, Generated, Sequencing, Negative Control, Plasmid Preparation, Software, Cell Analysis, Microscopy

Journal: Frontiers in Physiology

Article Title: Altered actin isoforms expression and enhanced airway responsiveness in asthma: the crucial role of β-cytoplasmic actin

doi: 10.3389/fphys.2025.1627443

Figure Lengend Snippet: Expression and localization of proteins in bronchial smooth muscle of guinea pigs from the asthma model. (A) Micrographs showing the localization of positive staining for bronchi proteins in guinea pigs from both the allergic asthma model and control groups. Positive staining in the airway smooth muscle (ASM) (red asterisk) and airway epithelium (blue asterisk) in both groups. Blank control micrographs correspond to background staining controls. Chromogen: DAB; counterstained with hematoxylin. Optical microscope, 40x magnification. (B) Pixel expression of the positive mark for proteins analyzed by immunohistochemistry. White bars represent the control group, and black bars represent the allergic asthma model. ACTG1: γ-actin, ACTB: actin-β, ACTA2: α-smooth muscle actin, MYH11: myosin heavy chain 11, MYL9: myosin light chain, FLNA: filamin A and TAGLN: transgelin. Analysis was performed using ImageJ-Fiji software, n = 5, unpaired Student's t-test, **p < 0.01.

Article Snippet: Filamin A (FLNA) , Mouse monoclonal filamin 1 antibody, Santa Cruz Biotechnology, sc-71118 (Clone 3fl80) , 1:250.

Techniques: Expressing, Staining, Control, Microscopy, Immunohistochemistry, Software

Journal: Frontiers in Physiology

Article Title: Altered actin isoforms expression and enhanced airway responsiveness in asthma: the crucial role of β-cytoplasmic actin

doi: 10.3389/fphys.2025.1627443

Figure Lengend Snippet: (A) Scatter plots illustrating the relationship between the expression levels of cytosolic proteins in bronchial smooth muscle, along with the Pearson correlation coefficients. (B) The interactome of proteins observed in the smooth muscle of bronchi in a guinea pig asthma model. Blue circles represent proteins with increased expression compared to control, grey circles represent proteins with no change, and red circles represent proteins with decreased expression. Blue lines indicate positive correlations between proteins, while red discontinue lines indicate negative correlations. Abbreviations are as follows: ACTG1: actin-γ1, ACTB: cytoplasmic actin-β, ACTA2: muscle actin-α, MYH11: myosin heavy chain 11, MYL9: myosin light chain 9, FLNA: filamin A, and TAGLN: transgelin. The interactome was constructed based on data obtained from the STRING: Functional Protein Association Networks program, 2023.

Article Snippet: Filamin A (FLNA) , Mouse monoclonal filamin 1 antibody, Santa Cruz Biotechnology, sc-71118 (Clone 3fl80) , 1:250.

Techniques: Expressing, Control, Construct, Functional Assay