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Image Search Results
Journal: Journal of Virology
Article Title: Evaluation of Cellular Determinants Required for In Vitro Xenotropic Murine Leukemia Virus-Related Virus Entry into Human Prostate Cancer and Noncancerous Cells
doi: 10.1128/JVI.00274-10
Figure Lengend Snippet: Differential expression of XPR1 gene in immortalized cell lines. Xpr1 expression in prostate cancer (A), immortalized prostate cell lines (B), and the primary epithelial cell line PrEC (C) was detected by RT-PCR. β-Actin expression was used as a loading control.
Article Snippet: In addition, we also used nonimmortalized fibroblast cell lines, Pt-N and Pt-C, which were derived from benign and malignant prostate tissues, respectively ( 39 ), and the established primary
Techniques: Quantitative Proteomics, Expressing, Reverse Transcription Polymerase Chain Reaction, Control
Journal: Journal of Virology
Article Title: Evaluation of Cellular Determinants Required for In Vitro Xenotropic Murine Leukemia Virus-Related Virus Entry into Human Prostate Cancer and Noncancerous Cells
doi: 10.1128/JVI.00274-10
Figure Lengend Snippet: XMRV pseudovirus infectivity in prostate cancer cell lines (A), immortalized prostate fibroblast cell lines (B), epithelial cell lines with or without Xpr1 transfection (C), smooth muscle cell lines (D) with or without Xpr1 transfection, and primary epithelial cells (PrEC) (E). Representative images in the fluorescence and bright fields were captured at ×20 magnification. The experiments were carried out twice, and each time the experiments were performed in triplicate. (F) The average levels of GFP expression in infected cells were quantified by FACS analysis. Xpr1 expression levels were determined by quantitative real-time RT-PCR (G) and Western blotting (H). Asterisks indicate statistical significance (P < 0.001).
Article Snippet: In addition, we also used nonimmortalized fibroblast cell lines, Pt-N and Pt-C, which were derived from benign and malignant prostate tissues, respectively ( 39 ), and the established primary
Techniques: Infection, Transfection, Fluorescence, Expressing, Quantitative RT-PCR, Western Blot