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Image Search Results
Journal: bioRxiv
Article Title: The Kaposi’s sarcoma-associated herpesvirus TBP mimic uses a non-canonical DNA binding mode to promote viral late gene transcription
doi: 10.1101/2025.08.25.672222
Figure Lengend Snippet: ORF24 requires interaction with Rpb1 and ORF34, as well as its CTD and vTBP to bind late promoters in infected cells. A) ORF24 protein levels at 48 hpr in complemented iSLK cell lines. B) ChIP-qPCR of indicated cell lines at 48 hpr was performed with HA-antibody. qPCR to detect the associated DNA was performed with promoter-specific primers for a representative late (K8.1) and early (ORF57) promoter. C) Proposed model of ORF24 recognition of late promoters in comparison to eukaryotic mechanism.
Article Snippet: Luciferase reporter plasmids K8.1 Pr pGL4.16 + Ori (Addgene 131038) and
Techniques: Infection, ChIP-qPCR, Comparison