ppv Search Results


mouse anti vp2 monoclonal antibody  (Bioss)
93
Bioss mouse anti vp2 monoclonal antibody
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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ppv teto sphccas9 gr ic a ef1α rtta ip  (Addgene inc)
92
Addgene inc ppv teto sphccas9 gr ic a ef1α rtta ip
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
Ppv Teto Sphccas9 Gr Ic A Ef1α Rtta Ip, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pv 0001  (DSMZ)
93
DSMZ pv 0001
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
Pv 0001, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ppv ef1a 2xnls cse2 cas6 cas3 ipa  (Addgene inc)
90
Addgene inc ppv ef1a 2xnls cse2 cas6 cas3 ipa
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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ppv h1 ccdb ef1α rih vector  (Addgene inc)
92
Addgene inc ppv h1 ccdb ef1α rih vector
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
Ppv H1 Ccdb Ef1α Rih Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ppvteto sphccas9 gr ic a ef1α rtta ih  (Addgene inc)
88
Addgene inc ppvteto sphccas9 gr ic a ef1α rtta ih
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
Ppvteto Sphccas9 Gr Ic A Ef1α Rtta Ih, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ppv ef1a 2xnls cas7 cas5 cse1 ipa  (Addgene inc)
91
Addgene inc ppv ef1a 2xnls cas7 cas5 cse1 ipa
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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ppv ef1α 2xnls cas7 cas5 8 cas8 ica vector  (Addgene inc)
90
Addgene inc ppv ef1α 2xnls cas7 cas5 8 cas8 ica vector
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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ppv ef1a 2xnls cse2 cas6 cas3 ica  (Addgene inc)
90
Addgene inc ppv ef1a 2xnls cse2 cas6 cas3 ica
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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ppv-a  (Lonza)
90
Lonza ppv-a
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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ppv  (Merck & Co)
90
Merck & Co ppv
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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mpmri sensitivity, ppv and npv  (ProMIS Neurosciences)
90
ProMIS Neurosciences mpmri sensitivity, ppv and npv
PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV <t>VP2,</t> ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .
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Image Search Results


PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV VP2, ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .

Journal: Veterinary Research

Article Title: Porcine parvovirus infection induces necroptosis of porcine placental trophoblast cells via a ZBP1-mediated pathway

doi: 10.1186/s13567-024-01410-x

Figure Lengend Snippet: PPV infection induces both apoptotic and necroptotic cell death in porcine PTCs . A – D Porcine PTCs were pretreated with the apoptosis inhibitor, Ac-DEVD-CHO, for 1 h and subsequently infected with PPV at a MOI of 1 for 0, 24, 48, and 72 h in the presence of Ac-DEVD-CHO. A Cellular LDH release levels were measured. B The MOCK- and PPV-infected cells were observed by transmission electron microscopy at 72 h. C The expression levels of PPV VP2, ZBP1, p-RIPK3, RIPK3, p-MLKL, and MLKL were analyzed by Western blotting. D The subcellular localization of MLKL in MOCK- and PPV-infected cells was visualized by confocal microscopy. E PTCs were pretreated with the necroptosis inhibitors (Nec-1, 10 μM; GSK'872, 20 μM; GW806742X, 20 nM) or same volume of DMSO for 4 h, respectively, and then infected with 1 MOI PPV for 0 h, 24 h, 48 h, and 72 h in the presence of these inhibitors. The copy numbers of PPV were measured by quantitative PCR. * P < 0.05, ** P < 0.01 versus PPV-infected cells at 0 h in A and C . * P < 0.01 versus DMSO-treated cells in E .

Article Snippet: Mouse anti-VP2 monoclonal antibody (bsm-41390M) was purchased from Bioss (Beijing, China).

Techniques: Infection, Transmission Assay, Electron Microscopy, Expressing, Western Blot, Confocal Microscopy, Real-time Polymerase Chain Reaction