Journal: Journal of Neuroinflammation

Article Title: Resolvin RvD2 reduces hypothalamic inflammation and rescues mice from diet-induced obesity

doi: 10.1186/s12974-016-0777-2

Figure Lengend Snippet: The impact of exogenous RvD2-injected icv on inflammatory and metabolic parameters in mice. Six-week-old Swiss mice were included in the study and fed a HF diet for 8 weeks before intracerebroventricular (icv) cannulation; after 1 week, mice were randomly selected for either saline (2 μl) or different amounts of RvD2 (2, 3 or 50 ng) icv treatment for 11 days ( a ). At the end of the experimental period, hypothalamic RNA was extracted and employed in real-time PCR determinations of GPR18 ( b ). Cumulative food intake ( c ) and body mass variation ( d ) were determined during the experimental period. At the end of the experimental period, the epididymal fat pad was measured ( e ). In addition, mice were submitted a glucose tolerance test, and results are expressed as the area under the curve (AUC) ( f ). Interleukin-6 (IL6) ( g ) and interleukin-10 (IL10) ( h ) transcripts were determined in samples from the hypothalamus, whereas uncoupling protein 1 (UCP1) ( i ) and PGC1a ( j ) transcripts were determined in samples from the brown adipose tissue. Some mice were subjected to indirect calorimetry, resulting in the values for O 2 consumption ( k ), CO 2 production ( l ) and respiratory quotient ( m ). In addition, some mice were subjected to a leptin tolerance test (LTT) and results are expressed as cumulative food intake during 12 h ( n ). In the experiments reported in panels a – j , n = 8; in the experiments reported in panels k – n , n = 5. * p < 0.05 vs. saline. Experiments for evaluation of 3 and 50 ng RvD2 in the hypothalamus were performed in different occasions. The differences obtained in some of the saline groups reflect interexperimental variability. For statistical analysis, group-specific saline control was considered as baseline

Article Snippet: TaqMan primers for PLA2 (Mm00448161_m1), 15-LOX (Mm00507789_m1), 5-LOX (Mm01182747_m1), GPR18 (Mm01224541_s1), TNFα (Mm00443258_m1), IL1β (Mm00434228_m1), IL6 (Mm00446190_m1), IL10 (Mm01288386_m1), PGC1α (Mm00447183_m1), UCP1 (Mm01244861_m1) and glyceraldehyde-3-phosphate dehydrogenase (GAPD) (#4352339E) were obtained from Applied Biosystems.

Techniques: Injection, Saline, Real-time Polymerase Chain Reaction, Control

Journal: PLoS ONE

Article Title: Aromatase deficiency in hematopoietic cells improves glucose tolerance in male mice through skeletal muscle-specific effects

doi: 10.1371/journal.pone.0227830

Figure Lengend Snippet: Primer sets employed.

Article Snippet: Cebpa , Mm00514283_s1 , Ppargc1a , Mm00447184_g1.

Techniques:

Journal: Pathology international

Article Title: FHL1: A novel diagnostic marker for papillary thyroid carcinoma.

doi: 10.1111/pin.13467

Figure Lengend Snippet: FIGURE 5 The expression of FHL1, BCL2, TLE1, KIT, PPARGC1A and GHR in papillary thyroid carcinoma (PTC) and the control group. FHL1 showed strong positivity in the nuclei and to a lesser extent in the cytoplasm of normal thyroid tissues adjacent to the tumour (the control group), but is not present in the PTC tissues. (p < 0.01). There was no significant difference in the expression of BCL2 between PTC and normal thyroid tissues (p > 0.05). The results showed that TLE1, KIT, PPARGC1A, and GHR were absent in both tumor and normal tissues. (magnification ×200). FHL1, four and a half LIM domains 1.

Article Snippet: Slides were immunostained with monoclonal antibodies against the BCL2 antigen (clone SP66, Maixin), CD117/C‐kit antigen (clone YR145, Maixin), and with monoclonal antibodies against the FHL1 (10991‐1‐AP, Proteintech Group, Inc.), PPARGC1A (Boster, China), and TLE1 antigen (Maixin).

Techniques: Expressing, Control