Journal: Frontiers in Nutrition

Article Title: Zhaqu compound improves glucose and lipid metabolism in T2DM with MASLD by modulating gut microbiota and PPARγ

doi: 10.3389/fnut.2026.1775686

Figure Lengend Snippet: ZQC regulates lipid metabolism via the PPARγ pathway in HG/PA-induced HepG2 cells. (A,B) Intracellular TG and T-CHO levels. (C,D) Relative expression of proteins (e.g., SREBP-1c, ACC1). (E–H) WB quantification of representative lipogenic and PPARγ pathway proteins (FASN, PPARγ, CD36, FABP4) in control, HG/PA, HG/PA + 5% ZQC-S, and HG/PA + 7.5% ZQC-S groups. (I) Representative Oil Red O staining images of lipid droplets. (J) Quantification of lipid droplet content (ORO%) in each group. (K,L) Intracellular TG and T-CHO levels. (M) WB analysis of key proteins in control, HG/PA, HG/PA + 7.5% ZQC-S, and HG/PA + 7.5% ZQC-S + GW1929 groups. (N–S) Relative expression of selected lipid metabolism and PPARγ pathway proteins across the four groups. ( # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HG/PA; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. HG/PA + 5% ZQC-S + g1929).

Article Snippet: The reagents and antibodies used were the Total Protein (TP) Assay Kit (1,000 tests, P0006, Biyuntian Biotechnology, China), Glucose Assay Kit (96 T, A154-1-1, Nanjing Jiancheng Bioengineering Institute, China), Total Cholesterol (T-CHO) Assay Kit (96 T, A111-1-1, Nanjing Jiancheng Bioengineering Institute, China), Triglyceride (TG) Assay Kit (96 T, A110-1-1, Nanjing Jiancheng Bioengineering Institute, China), Sequencing Reagent Kit (NovaSeq 6,000 SP Reagent Kit V1.5, Illumina, USA), RNA Mini Kit (Qiagen, Germany), DMEM High Glucose Medium (PM150210, Procell, China), Trypsin (S310JV, Shanghai Yuanpei, China), Fetal Bovine Serum (C04001-500, Vivacell, China), Double Antibody (S110JV, Shanghai Yuanpei, China), PPARγ agonist (HY-146480, MCE, USA), Palmitic acid (H8780, Solarbio, China), Oil Red O staining kit (G1262, Solarbio, China), 2-NBDG fluorescent probe (HY-116215, MCE, USA), BCA protein concentration assay kit (BL521C, Biosharp, China), SDS-PAGE Protein Loading Buffer (5×) (BL502A, Biosharp, China), ECL Chemiluminescent Substrate (BL520B, Biosharp, China), Western Blot & IP Cell Lysis Buffer (P0013, Beyotime, China), PBS Buffer ( PB180327 , Procell, China), and the primary antibodies β -Microtubulin (AC026, Abclonal, China), PEPCK (ET7107-29, Huabio, China), G6Pase (A21168, Abclonal, China), GLUT2 (A12307, Abclonal, China), SREBP-1C (ER1917-19, Huabio, China), ACC1 (ET1609-77, Huabio, China), FASN (R1706-8, Huabio, China), PPARγ (A19676, Abclonal, China), CD36 (ET1701-24, Huabio, China), and FABP4 (ET1703-98, Huabio, China).

Techniques: Expressing, Control, Staining

Journal: Journal of medicinal chemistry

Article Title: A novel N-substituted valine derivative with unique PPARγ binding properties and biological activities

doi: 10.1021/acs.jmedchem.0c01555

Figure Lengend Snippet: Effect of 7j on PPARγ phosphorylation. (A) Percentage of in vitro PPARγ Ser273 phosphorylation by CDK5 in the presence of 0.1 μM of 7j or Rosi. (B) Phosphorylation of PPARγ Ser273 in 3T3-L1 adipocyte incubated for 60 minutes with 5 μM of Rosi, 7j or Roscovitine (Rsv) before TNFα stimulation (50 ng/ml; 90 minutes). Two independent experiments are shown. Phosphospecific antibodies were from Rockland or New England Peptide (NEP). Arrow heads indicate phosphorylated PPARγ and PPARγ2. (C) RT-PCR analysis of the expression levels of a selection of genes known to be regulated by CDK5-dependent phosphorylation of PPARγ. Values are means ± SD (n = 5) expressed relative to the mean of control. **p < 0.01, ***p < 0.001 vs. control (one-way ANOVA followed by Dunnett’s post hoc test).

Article Snippet: PPARγ phospho Ser273 antibodies were from Rockland (Limerick, PA, USA) or custom produced by New England Peptide (Gardner, MA, USA).

Techniques: Phospho-proteomics, In Vitro, Incubation, Reverse Transcription Polymerase Chain Reaction, Expressing, Selection, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effect of Actin Alpha Cardiac Muscle 1 on the Proliferation and Differentiation of Bovine Myoblasts and Preadipocytes

doi: 10.3390/ani11123468

Figure Lengend Snippet: RT-qPCR primer sequence.

Article Snippet: The protein samples in the gel were transferred to a polyvinylidene fluoride membrane (PVDF, Merck Millipore, Taufkirchen, Germany), and then sealed with QuickBlock Western blocking solution (Beyotime Biotechnology, Shanghai, China) for 20 min. GAPDH (rabbit anti-GAPDH, 1:10,000 Abcam, Cambridge, UK, NP_001029206.1), ACTC1 (rabbit anti-ACTC1, 1:1000, Invitrogen, CA, USA NP_001029757.1), MYOD1 (mouse anti-MYOD1, 1:1000, Abcam, Cambridge, NT, UK, NP_001035568.2), MYOG (rabbit anti-MYOG, 1:1000, Abcam, Cambridge, NT, UK, NP_001104795.1), MYHC (mouse anti-MYHC, 1:200, Abcam, Cambridge, NT, UK), MRF4 (mouse anti-MYF6, 1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA, NP_861527.1), MEF2A (rabbit anti-MEF2A, 1:1000, Abcam, Cambridge, NT, UK, NP_001077107.1), FABP4 (rabbit anti-FABP4, 1:1000, Abcam, NT, UK, NP_776739.1), PPARγ (rabbit anti-PPARγ, 1:1000, Boster, Wuhan, China, NP_851367.1), FASN (rabbit anti-FASN, 1:2000, Abcam, Cambridge, NT, UK, NP_777087.1) antibodies were added and incubated overnight at 4 °C.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effect of Actin Alpha Cardiac Muscle 1 on the Proliferation and Differentiation of Bovine Myoblasts and Preadipocytes

doi: 10.3390/ani11123468

Figure Lengend Snippet: Detection of adipogenic marker gene expression at mRNA level after ectopic expression of ACTC1 . ( a – e ) Detection of mRNA expression of ACTC1 , PPARγ , FABP4 , FASN and SCD1 genes on D0, D2, D4, D6 and D8 days by qRT-PCR after ectopic expression ACTC1 . Each experiment was performed in triplicate. Error bars represent s.e.m. * p < 0.05; ** p < 0.01.

Article Snippet: The protein samples in the gel were transferred to a polyvinylidene fluoride membrane (PVDF, Merck Millipore, Taufkirchen, Germany), and then sealed with QuickBlock Western blocking solution (Beyotime Biotechnology, Shanghai, China) for 20 min. GAPDH (rabbit anti-GAPDH, 1:10,000 Abcam, Cambridge, UK, NP_001029206.1), ACTC1 (rabbit anti-ACTC1, 1:1000, Invitrogen, CA, USA NP_001029757.1), MYOD1 (mouse anti-MYOD1, 1:1000, Abcam, Cambridge, NT, UK, NP_001035568.2), MYOG (rabbit anti-MYOG, 1:1000, Abcam, Cambridge, NT, UK, NP_001104795.1), MYHC (mouse anti-MYHC, 1:200, Abcam, Cambridge, NT, UK), MRF4 (mouse anti-MYF6, 1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA, NP_861527.1), MEF2A (rabbit anti-MEF2A, 1:1000, Abcam, Cambridge, NT, UK, NP_001077107.1), FABP4 (rabbit anti-FABP4, 1:1000, Abcam, NT, UK, NP_776739.1), PPARγ (rabbit anti-PPARγ, 1:1000, Boster, Wuhan, China, NP_851367.1), FASN (rabbit anti-FASN, 1:2000, Abcam, Cambridge, NT, UK, NP_777087.1) antibodies were added and incubated overnight at 4 °C.

Techniques: Marker, Gene Expression, Expressing, Quantitative RT-PCR

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effect of Actin Alpha Cardiac Muscle 1 on the Proliferation and Differentiation of Bovine Myoblasts and Preadipocytes

doi: 10.3390/ani11123468

Figure Lengend Snippet: Detection of adipogenic marker gene expression at protein level after ectopic expression of ACTC1 . ( a ) Detect the proteins expression of ACTC1, PPARγ, FABP4, FASN and SCD1 on D2, D4, D6 and D8 days by Western blot after ectopic expression of ACTC1 . ( b – f ) Use Image J software to measure the gray value of protein bands. Each experiment was performed in triplicate. Error bars represent s.e.m. * p < 0.05; ** p < 0.01. Original western blot figures in .

Article Snippet: The protein samples in the gel were transferred to a polyvinylidene fluoride membrane (PVDF, Merck Millipore, Taufkirchen, Germany), and then sealed with QuickBlock Western blocking solution (Beyotime Biotechnology, Shanghai, China) for 20 min. GAPDH (rabbit anti-GAPDH, 1:10,000 Abcam, Cambridge, UK, NP_001029206.1), ACTC1 (rabbit anti-ACTC1, 1:1000, Invitrogen, CA, USA NP_001029757.1), MYOD1 (mouse anti-MYOD1, 1:1000, Abcam, Cambridge, NT, UK, NP_001035568.2), MYOG (rabbit anti-MYOG, 1:1000, Abcam, Cambridge, NT, UK, NP_001104795.1), MYHC (mouse anti-MYHC, 1:200, Abcam, Cambridge, NT, UK), MRF4 (mouse anti-MYF6, 1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA, NP_861527.1), MEF2A (rabbit anti-MEF2A, 1:1000, Abcam, Cambridge, NT, UK, NP_001077107.1), FABP4 (rabbit anti-FABP4, 1:1000, Abcam, NT, UK, NP_776739.1), PPARγ (rabbit anti-PPARγ, 1:1000, Boster, Wuhan, China, NP_851367.1), FASN (rabbit anti-FASN, 1:2000, Abcam, Cambridge, NT, UK, NP_777087.1) antibodies were added and incubated overnight at 4 °C.

Techniques: Marker, Gene Expression, Expressing, Western Blot, Software