Journal: Frontiers in Immunology

Article Title: miR-223 alleviates DSS-induced colitis by prompting macrophage M2 polarization through PPAR-γ/FOXO1 signaling

doi: 10.3389/fimmu.2025.1598781

Figure Lengend Snippet: Relieving colitis by miR-223 through the promotion of macrophage M2 polarization via the modulation of PPAR-γ/FOXO1 signaling. (A) Representative images of western blots indicating the protein expression levels of PPAR-γ and FOXO1. The data represent the findings from three independent experiments. (B, C) Changes in the expression of PPAR-γ and FOXO1. (D, E) Changes in the mRNA levels of PPAR-γ and FOXO1. All the data are expressed as the means ± SDs (n=6 each group). *p<0.05, **p<0.01, ***p<0.001. (F, G) Correlations of colonic miR-223 expression with PPAR-γ and FOXO1 expression in the colon. Pearson analyses were used to correlate miR-223 with colonic PPAR-γ and FOXO1 expression. PPAR-γ, peroxisome proliferator-activated receptor gamma; FOXO1, Forkhead box transcription factor O1.

Article Snippet: After being blocked with bovine serum albumin blocking buffer (3% in PBS), the slides were incubated with the following primary antibodies: anti-PPAR-γ antibody (1:3000) (Cat.# GB11163, Servicebio, Wuhai, China), anti-FOXO1 antibody (1:10000) (Cat.# GB11286-1, Servicebio), anti-CD206 (1:5000) (Cat.# GB113497 , Servicebio), anti-CD86 (1:5000) (Cat.# GB115630 , Servicebio) or anti-F4/80 (1:2000) (Cat.# GB113373 , Servicebio) overnight at 4°C.

Techniques: Western Blot, Expressing

Journal: Frontiers in Immunology

Article Title: miR-223 alleviates DSS-induced colitis by prompting macrophage M2 polarization through PPAR-γ/FOXO1 signaling

doi: 10.3389/fimmu.2025.1598781

Figure Lengend Snippet: Immunofluorescence analysis of PPAR-γ and M2 macrophage marker CD206 in colonic tissue. Representative micrographs depict protein expression levels of PPAR-γ (red) and its co-localization (yellow) with CD206 (green) in colon sections. Nuclei were counterstained with DAPI (blue). Scale bar: 100 µm.

Article Snippet: After being blocked with bovine serum albumin blocking buffer (3% in PBS), the slides were incubated with the following primary antibodies: anti-PPAR-γ antibody (1:3000) (Cat.# GB11163, Servicebio, Wuhai, China), anti-FOXO1 antibody (1:10000) (Cat.# GB11286-1, Servicebio), anti-CD206 (1:5000) (Cat.# GB113497 , Servicebio), anti-CD86 (1:5000) (Cat.# GB115630 , Servicebio) or anti-F4/80 (1:2000) (Cat.# GB113373 , Servicebio) overnight at 4°C.

Techniques: Immunofluorescence, Marker, Expressing

Journal: Frontiers in Immunology

Article Title: miR-223 alleviates DSS-induced colitis by prompting macrophage M2 polarization through PPAR-γ/FOXO1 signaling

doi: 10.3389/fimmu.2025.1598781

Figure Lengend Snippet: Schematic representation of the potential mechanisms underlying the targeted therapy of miR-223 supplement for DSS-induced colitis. miR-223 ameliorates DSS-induced colitis through promoting macrophage M2 polarization via modulation of PPAR-γ and FOXO1 signaling. DSS, dextran sodium sulfate.

Article Snippet: After being blocked with bovine serum albumin blocking buffer (3% in PBS), the slides were incubated with the following primary antibodies: anti-PPAR-γ antibody (1:3000) (Cat.# GB11163, Servicebio, Wuhai, China), anti-FOXO1 antibody (1:10000) (Cat.# GB11286-1, Servicebio), anti-CD206 (1:5000) (Cat.# GB113497 , Servicebio), anti-CD86 (1:5000) (Cat.# GB115630 , Servicebio) or anti-F4/80 (1:2000) (Cat.# GB113373 , Servicebio) overnight at 4°C.

Techniques:

Journal: Heliyon

Article Title: Pharmacological investigation of taxifolin for its therapeutic potential in depression

doi: 10.1016/j.heliyon.2024.e30467

Figure Lengend Snippet: The best conformational pose, binding energy (kcal/mol), number of hydrogen bonds, bonding residues forming other hydrophobic interactions, of taxifolin and fluoxetine with target proteins such as peroxisome proliferator-activated receptor gamma (PPAR-γ), cyclooxygenase-2 (COX-2), Toll like receptor-4 (TLR4), c-Jun N-terminal kinase (JNK), brain-derived neurotrophic factor (BDNF), monoamine oxidase A (MAO-A), heme oxygenase-1 (HO-1), cyclooxygenase-1 (COX-1), sodium channels (NA+),Glutamate receptor (GRM2), phosphoinositide 3-kinase (PI3k), tumor necrosis factor-alpha (TNF-α), prostaglandins (PGE2), mitogen activated protein kinases (MAPK), Beta- 2 adrenergic receptor (ADRB2), Neurokinin receptor (NK-1), Procaspase activating compound (PAC-1), nuclear factor kappa B (NF-κB), nitric oxidase synthesis (iNOS), interleukin-4 (IL-4), high mobility group box 1 (HMGB1), Protein -c –fos, Beta catenin (β-Catenin), serotonin receptors (SERT), nuclear factor erythroid 2-related factor 2 (Nrf2), vasoactive intestinal peptide (VIP), Gamma-aminobutyric acid (GABA A), peptidoglycan (PG), interleukin-2 (IL-2), Dopamine receptor (D2).

Article Snippet: The ELISA kit COX-2 (CAT# PRS-30205Ra) and the ELISA kit PPAR gamma (CAT# E-EL-R0724) were supplied by Elabscience.

Techniques: Binding Assay

Journal: Heliyon

Article Title: Pharmacological investigation of taxifolin for its therapeutic potential in depression

doi: 10.1016/j.heliyon.2024.e30467

Figure Lengend Snippet: Effects of Taxifolin and Fluoxetine against (A) Peroxisomes proliferation-activated receptor-γ (PPAR-γ) and (B) Cyclooxygenase-2 (COX-2) concentration in rat's cortex tissues using enzyme linked immunosorbent assay technique (ELISA). Values expressed as mean ± SEM (n = 6). One-way ANOVA with post hoc Tukey’ s test. ### P < 0.001 vs. saline group, *P < 0.05, **P < 0.01, ***P < 0.001 vs. LPS group.

Article Snippet: The ELISA kit COX-2 (CAT# PRS-30205Ra) and the ELISA kit PPAR gamma (CAT# E-EL-R0724) were supplied by Elabscience.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Saline

Journal: Heliyon

Article Title: Pharmacological investigation of taxifolin for its therapeutic potential in depression

doi: 10.1016/j.heliyon.2024.e30467

Figure Lengend Snippet: Effects of Taxifolin and Fluoxetine against Peroxisomes proliferation-activated receptor-γ (PPAR-γ) by RT-PCR. Values expressed as mean ± SEM (n = 6). One-way ANOVA with post hoc Tukey’ s test. ### P < 0.001 vs saline group, **P < 0.01, ***P < 0.001 vs. LPS group.

Article Snippet: The ELISA kit COX-2 (CAT# PRS-30205Ra) and the ELISA kit PPAR gamma (CAT# E-EL-R0724) were supplied by Elabscience.

Techniques: Reverse Transcription Polymerase Chain Reaction, Saline

Journal: PLoS ONE

Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

doi: 10.1371/journal.pone.0045514

Figure Lengend Snippet: Oligonucleotides and plasmids.

Article Snippet: pSV Sport PPARγ , PPARγ expression plasmid , Addgene 8886, .

Techniques: Luciferase, Plasmid Preparation, Expressing, Control, Dominant Negative Mutation

Journal: PLoS ONE

Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

doi: 10.1371/journal.pone.0045514

Figure Lengend Snippet: Flotillin promoter constructs F1-1330 (A, C) or F2-2130 (B, D) were cotransfected into Hela cells together with expression plasmids for RAR, RXR, PPARγ or with empty PSV control plasmid. One day post-transfection, the cells were stimulated with trans-RA (1 µM) for 24 h in serum-free medium. Relative luciferase activity of the unstimulated control sample was set as 1. F1-1330 (E) and F2-2130 (F) transfected Hela cells were stimulated with troglitazone for 24 h in serum-free medium. Values are mean ± standard deviation of at least 3 experiments measured in duplicates. ***p<0.001; **p<0.01; *p<0.05 vs. respective control.

Article Snippet: pSV Sport PPARγ , PPARγ expression plasmid , Addgene 8886, .

Techniques: Construct, Expressing, Control, Plasmid Preparation, Transfection, Luciferase, Activity Assay, Standard Deviation

Journal: PLoS ONE

Article Title: Transcriptional Regulation of Flotillins by the Extracellularly Regulated Kinases and Retinoid X Receptor Complexes

doi: 10.1371/journal.pone.0045514

Figure Lengend Snippet: Hela cells were transiently transfected with expression constructs for RAR, RXR, or a combination of both. Empty PSV vector served as a control. One day post-transfection, the cells were stimulated with trans-RA (1 µM) in serum-free medium for 24 h. Cell lysates were analyzed for flotillin-1 (A), flotillin-2 (B), RAR and RXR (C) by Western blotting. D and E show a densitometric quantification of flotillin expression. F: Cells were transfected with RAR or PPARγ expression construct or empty PSV. RNA was isolated, transcribed into cDNA and flotillin mRNA was measured by qPCR. Values are mean ± standard deviation of at least 3 experiments. ###, p<0.001; #, p<0.05; vs control *, p<0.05 vs. unstimulated sample.

Article Snippet: pSV Sport PPARγ , PPARγ expression plasmid , Addgene 8886, .

Techniques: Transfection, Expressing, Construct, Plasmid Preparation, Control, Western Blot, Isolation, Standard Deviation

Journal: British journal of pharmacology

Article Title: Inactivation of SERCA2 Cys 674 accelerates aortic aneurysms by suppressing PPARγ.

doi: 10.1111/bph.15411

Figure Lengend Snippet: FIGURE 1 Inactivation of SERCA2 C674 suppresses PPARγ by activation of calcineurin/ NFAT/NF-κB pathways. (a) The main signalling pathways from the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis in duplicate aorta samples of WT and SKI mice in LDLR−/−background. (b) The mRNA levels of PPARγ2 in aortas of WT and SKI mice in LDLR−/−

Article Snippet: The PPARγ2 plasmids (ORIGENE, Cat# MR208132) or its control CMV-GFP plasmids (ORIGENE, Cat# PS100040) were transfected into SKI SMCs according to Effectene® Transfection Reagent Handbook (Qiagen, Cat#301305).

Techniques: Activation Assay

Journal: British journal of pharmacology

Article Title: Inactivation of SERCA2 Cys 674 accelerates aortic aneurysms by suppressing PPARγ.

doi: 10.1111/bph.15411

Figure Lengend Snippet: FIGURE 3 The down-regulation of PPARγ2 accounts for the phenotypic modulation of SKI SMCs. (a) Representative western blots from SKI aortic SMCs transfected with PPARγ2 plasmids and quantification of band intensities in graph. Data shown are means ± SEM; n = 5. *P < .05, significantly different as indicated; unpaired Student's t test.. (b) Cell proliferation. (c) Cell migration. (d) Macrophage adhesion to aortic SMCs. In (b–d), data shown are means ± SEM; n = 6. *P < .05, significantly different as indicated; unpaired Student's t test

Article Snippet: The PPARγ2 plasmids (ORIGENE, Cat# MR208132) or its control CMV-GFP plasmids (ORIGENE, Cat# PS100040) were transfected into SKI SMCs according to Effectene® Transfection Reagent Handbook (Qiagen, Cat#301305).

Techniques: Western Blot, Transfection, Migration

Journal: Journal of medicinal chemistry

Article Title: A novel N-substituted valine derivative with unique PPARγ binding properties and biological activities

doi: 10.1021/acs.jmedchem.0c01555

Figure Lengend Snippet: Effect of 7j on PPARγ phosphorylation. (A) Percentage of in vitro PPARγ Ser273 phosphorylation by CDK5 in the presence of 0.1 μM of 7j or Rosi. (B) Phosphorylation of PPARγ Ser273 in 3T3-L1 adipocyte incubated for 60 minutes with 5 μM of Rosi, 7j or Roscovitine (Rsv) before TNFα stimulation (50 ng/ml; 90 minutes). Two independent experiments are shown. Phosphospecific antibodies were from Rockland or New England Peptide (NEP). Arrow heads indicate phosphorylated PPARγ and PPARγ2. (C) RT-PCR analysis of the expression levels of a selection of genes known to be regulated by CDK5-dependent phosphorylation of PPARγ. Values are means ± SD (n = 5) expressed relative to the mean of control. **p < 0.01, ***p < 0.001 vs. control (one-way ANOVA followed by Dunnett’s post hoc test).

Article Snippet: PPARγ phospho Ser273 antibodies were from Rockland (Limerick, PA, USA) or custom produced by New England Peptide (Gardner, MA, USA).

Techniques: Phospho-proteomics, In Vitro, Incubation, Reverse Transcription Polymerase Chain Reaction, Expressing, Selection, Control

Journal: Journal of Animal and Feed Sciences

Article Title: β-carotene as a dietary factor affecting expression of genes connected with carotenoid, vitamin A and lipid metabolism in the subcutaneous and omental adipose tissue of beef cattle

doi: 10.22358/jafs/117866/2020

Figure Lengend Snippet: Figure 3. Expression of BCMO1 (A), PPARγ (B) and RXRα (C) protein in omental fat of beef cattle fed different doses of β-carotene (βC) (0, 600, 1200 and 1800 mg/d); Figure A, B and C: bars present means ± standard error (SE), for 3 steers per group; a–c – bars with different superscripts vary significantly (P < 0.05); Figure D: bands present one representative sample from each group

Article Snippet: The primary antibodies were chosen as follows: anti-BCMO1 polyclonal antibodies (1:200, sc-163736; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-PPARγ polyclonal antibodies (1:400, NB120-19481; Novus Biologicals, Littleton, CO, USA), anti-RXRα monoclonal antibodies (1:1000, LS-C80051; LifeSpan BioSciences, Inc., Seattle, WA, USA), and anti-β-actin monoclonal antibodies (1:1000, 4970S; Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Expressing

Journal: Journal of Animal and Feed Sciences

Article Title: β-carotene as a dietary factor affecting expression of genes connected with carotenoid, vitamin A and lipid metabolism in the subcutaneous and omental adipose tissue of beef cattle

doi: 10.22358/jafs/117866/2020

Figure Lengend Snippet: Figure 2. Expression of BCMO1 (A), PPARγ (B) and RXRα (C) protein in subcutaneous fat of beef cattle fed different doses of β-carotene (βC) (0, 600, 1200 and 1800 mg/d); Figure A, B and C: bars present means ± standard error (SE), for 3 steers per group; a–b – bars with different superscripts vary significantly (P < 0.05); Figure D: bands present one representative sample from each group

Article Snippet: The primary antibodies were chosen as follows: anti-BCMO1 polyclonal antibodies (1:200, sc-163736; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-PPARγ polyclonal antibodies (1:400, NB120-19481; Novus Biologicals, Littleton, CO, USA), anti-RXRα monoclonal antibodies (1:1000, LS-C80051; LifeSpan BioSciences, Inc., Seattle, WA, USA), and anti-β-actin monoclonal antibodies (1:1000, 4970S; Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Expressing