pp1 Search Results


1 naphthyl pp1  (MedChemExpress)
91
MedChemExpress 1 naphthyl pp1
1 Naphthyl Pp1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pp1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology pp1
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Pp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pp 1  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology pp 1
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Pp 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pp 1  (Tocris)
93
Tocris pp 1
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Pp 1, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pp1  (Addgene inc)
93
Addgene inc pp1
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Pp1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ab40877  (R&D Systems)
91
R&D Systems ab40877
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Ab40877, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 naphthyl pp1  (Tocris)
93
Tocris 1 naphthyl pp1
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
1 Naphthyl Pp1, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 naphthyl pp1/product/Tocris
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1 1 dimethylethyl  (Tocris)
93
Tocris 1 1 dimethylethyl
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
1 1 Dimethylethyl, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against tubulin ab11304  (R&D Systems)
93
R&D Systems antibodies against tubulin ab11304
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Antibodies Against Tubulin Ab11304, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti human pp1α catalytic subunit  (R&D Systems)
88
R&D Systems mouse monoclonal anti human pp1α catalytic subunit
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Mouse Monoclonal Anti Human Pp1α Catalytic Subunit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pp1α  (R&D Systems)
94
R&D Systems pp1α
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Pp1α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pp1  (Selleck Chemicals)
93
Selleck Chemicals pp1
Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and <t>PP1.</t> ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .
Pp1, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and PP1. ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .

Journal: Scientific Reports

Article Title: 4β-Hydroxywithanolide E Modulates Alternative Splicing of Apoptotic Genes in Human Hepatocellular Carcinoma Huh-7 Cells

doi: 10.1038/s41598-017-07472-6

Figure Lengend Snippet: Effect of 4bHWE on AKT kinase, SRPK1, SRPK2, and PP1. ( a ) Huh-7 cells were treated with the indicated concentration of 4bHWE for 24 h. The proteins were analyzed through Western blotting. ( b ) and ( c ) Huh-7 cells were treated with 5 μM AKT inhibitor for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. ( d ) and ( e ) Huh-7 cells were treated with 20 nM okadaic acid for 1 h and then exposed to 10 μM 4bHWE for 24 h. The proteins and mRNA were analyzed through Western blotting and RT-PCR, respectively. Western blotting: equal amounts of whole cell lysates (20 μg) were separated using SDS-PAGE and immunoblotted with various antibodies as indicated. Tubulin and Histone H3 are shown as internal standards. The fold-change values are presented below each band. RT-PCR: mRNA was extracted and detected for the alternative splicing of the HIPK3 , SMAC / DIABLO , and SURVIVIN transcripts. The ratios of the densities of the two bands (alternative exon-containing isoforms to alternative exon-lacking isoforms) are presented below each group. Data are the mean of three independent experiments. * p < 0.05 compared with the control. a p < 0.05 for the comparison between 4bHWE and AKT inhibitor + 4bHWE. b p < 0.05 for the comparison between 4bHWE and okadaic acid + 4bHWE. AI = AKT inhibitor; OA = okadaic acid; M = marker. Full-length blots are presented in Supplementary Fig. .

Article Snippet: Antibodies were purchased from the following companies: anti-H3K27me1, H3K27me3, H3K79me1, H3K79me2, histone H3, SETD2, and tubulin from Abcam; anti-hnRNP A2/B1 from Acris; anti-SRPK1 and SRPK2 from BD Biosciences; anti-AKT, H3K27me2, PP1, phospho-PP1 at Thr320, phospho-AKT at Ser473, and cleaved-CASPASE-3 from Cell Signaling Technology; anti-H3K4me1, H3K4me2, H3K4me3, and H3K36me3 from Millipore; anti-pro CASPASE-3 from GeneTex; anti-hnRNP C1/C2, BAX, BCL-2, and PARP from Santa Cruz Biotechnology; anti-hnRNP A1, SRSF6, and TRA2B from Sigma; and anti-SRSF1 and SRSF3 from Zymed.

Techniques: Concentration Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, SDS Page, Alternative Splicing, Control, Comparison, Marker