Journal: Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)

Article Title: FORMATION AND ROLE OF BACTERIAL COMMUNITY IN THE SEDIMENT BED OF OPEN CHANNEL RECEIVING JOHKASOU EFFLUENT: MULTIVARIATE STATISTICAL ANALYSIS INTERPRETATION

doi: 10.2208/jscejer.68.iii_1

Figure Lengend Snippet: Fig. 2 Box plots showing the spatial variation of sediment bed quality parameters of HPC_S, DNA_S and TS.

Article Snippet: A given quantity of sediment was subjected to DNA extraction using Power Soil TM DNA isolation kit (MO BIO Lab. Inc., CA) according to manufacturer’s instructions.

Techniques:

Journal: Micromachines

Article Title: Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device

doi: 10.3390/mi10060400

Figure Lengend Snippet: ( a ) Schematic of the designed microfluidic device to generate physical stimulations for lung epithelial cell surfactant protein expression studies. ( b ) Photo the fabricated device filled with colored food dyes (red: cell culture channel; blue: actuation channel.

Article Snippet: This paper reports a simple, but powerful, biomimetic microfluidic device capable of generating hydrostatic pressure and cyclic strain to provide a platform mimicking the lung physiological physical microenvironment for in vitro cell studies.

Techniques: Expressing, Cell Culture

Journal: Micromachines

Article Title: Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device

doi: 10.3390/mi10060400

Figure Lengend Snippet: ( a ) Schematic and ( b ) photo of the experimental setup of the microfluidic cell culture device capable of mimicking physical stimulations within lungs.

Article Snippet: This paper reports a simple, but powerful, biomimetic microfluidic device capable of generating hydrostatic pressure and cyclic strain to provide a platform mimicking the lung physiological physical microenvironment for in vitro cell studies.

Techniques: Cell Culture

Journal: Micromachines

Article Title: Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device

doi: 10.3390/mi10060400

Figure Lengend Snippet: The microscopic images of the A549 cells ( a ) right after seeding into the microfluidic device, and ( b ) after culturing in the microfluidic device overnight. After the overnight culture, the A549 cells attach well onto the cell culture chamber substrate. ( c ) Bright field phase and live (green)/dead (red)/nuclei (blue) fluorescence images of the A549 cells inside the culture chamber after 24-h cell experiments under the 4 different conditions. Device A: control experiment in the growth medium; Device B: cell culture in the growth medium containing 1 μM dexamethasone; Device C: cell culture in the growth medium with the physical stimulations; and Device D: cell culture under the combination of dexamethasone treatment and physical stimulations. The scale bars are 200 μm.

Article Snippet: This paper reports a simple, but powerful, biomimetic microfluidic device capable of generating hydrostatic pressure and cyclic strain to provide a platform mimicking the lung physiological physical microenvironment for in vitro cell studies.

Techniques: Cell Culture, Fluorescence, Control

Journal: Micromachines

Article Title: Study Effects of Drug Treatment and Physiological Physical Stimulation on Surfactant Protein Expression of Lung Epithelial Cells Using a Biomimetic Microfluidic Cell Culture Device

doi: 10.3390/mi10060400

Figure Lengend Snippet: ( a ) Bright field phase and fluorescence images of the A549 cells cultured within the microfluidic devices with different combinations of steroid treatment and physical stimulation. Device A: neither steroid treatment nor physical stimulation (control); Device B: only steroid treatment; Device C: only physical stimulation; Device D: steroid treatment and physical stimulation. ( b , c ) Normalized average fluorescence intensity showing SPC expression of the A549 cells after 12- and 24-h experiments under the four different conditions, respectively. The fluorescence intensities showing SPC expression levels with statistically significantly different are designated with different letters (a, b, c, d = p < 0.05).

Article Snippet: This paper reports a simple, but powerful, biomimetic microfluidic device capable of generating hydrostatic pressure and cyclic strain to provide a platform mimicking the lung physiological physical microenvironment for in vitro cell studies.

Techniques: Fluorescence, Cell Culture, Control, Expressing