pos Search Results


voltage controlled oscillators vcos  (Mini-Circuits)
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Mini-Circuits voltage controlled oscillators vcos
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pos  (Quest Diagnostics)
86
Quest Diagnostics pos
Pos, supplied by Quest Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n a h3histone pos cntrl human f 5  (Azenta)
86
Azenta n a h3histone pos cntrl human f 5
N A H3histone Pos Cntrl Human F 5, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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am00143pu n  (OriGene)
90
OriGene am00143pu n
Western blot primary and secondary antibodies, dilutions and company/catalog information.
Am00143pu N, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lc3b  (OriGene)
90
OriGene lc3b
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Lc3b, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5-μg pos paper disk  (Becton Dickinson)
90
Becton Dickinson 5-μg pos paper disk
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
5 μg Pos Paper Disk, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pos(icrc2017)  (MediaLab Inc)
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MediaLab Inc pos(icrc2017)
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Pos(icrc2017), supplied by MediaLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive displacement pipets rainin microman  (METTLER TOLEDO)
90
METTLER TOLEDO positive displacement pipets rainin microman
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Positive Displacement Pipets Rainin Microman, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive displacement pipette tips  (METTLER TOLEDO)
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METTLER TOLEDO positive displacement pipette tips
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Positive Displacement Pipette Tips, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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equifax's pos  (Equifax Inc)
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Equifax Inc equifax's pos
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Equifax's Pos, supplied by Equifax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pos av610 system  (Applanix Corporation)
90
Applanix Corporation pos av610 system
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Pos Av610 System, supplied by Applanix Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ganges s19202 sts-192 pos/atm sonet/sdh mapper  (Applied Micro Circuits Corporation)
90
Applied Micro Circuits Corporation ganges s19202 sts-192 pos/atm sonet/sdh mapper
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing <t>EGFP-LC3B</t> were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD
Ganges S19202 Sts 192 Pos/Atm Sonet/Sdh Mapper, supplied by Applied Micro Circuits Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Western blot primary and secondary antibodies, dilutions and company/catalog information.

Journal: Reproduction (Cambridge, England)

Article Title: Perinatal protein restriction with postnatal catch-up growth leads to elevated p66Shc and mitochondrial dysfunction in the adult rat liver

doi: 10.1530/REP-19-0188

Figure Lengend Snippet: Western blot primary and secondary antibodies, dilutions and company/catalog information.

Article Snippet: SHC1 , Mouse monoclonal , 1:1000 , Acris Antibodies, Rockville, MD, USA (AM00143PU-N).

Techniques: Western Blot, Marker

a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing EGFP-LC3B were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD

Journal: Cell Death & Disease

Article Title: BAG3 promotes autophagy and glutaminolysis via stabilizing glutaminase

doi: 10.1038/s41419-019-1504-6

Figure Lengend Snippet: a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing EGFP-LC3B were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. * P < 0.05. Error bars indicate means ± SD

Article Snippet: Antibodies were used against the following: GLS (Sigma-Aldrich, WH0002744M1), GLS (Abcam, ab156876), GAPDH (Merck-Millipore, AB2302), LC3B (Origene, am20213pu), P62 (BD Biosciences, 610833), Beclin1 (Cell Signaling Technology, 3495), ATG3 (Cell Signaling Technology, 3415), ATG5 (Cell Signaling Technology, 12994), ATG7 (Cell Signaling Technology, 8558), ATG12 (Cell Signaling Technology, 4180), SIRT5 (Cell Signaling Technology, 8779), c-Myc (Invitrogen, R950-25), DYKDDDDK Tag (Cell Signaling Technology, 14793), HA-Tag (Cell Signaling Technology, 3724), pan-succinylation (PTM Biolabs, Hangzhou).

Techniques: Infection, Construct, Expressing, Western Blot, Stable Transfection, Microscopy, Transduction, Transmission Assay, Electron Microscopy

a Control or BAG3-overexpressing HepG2 and MCF7 cells were transfected with scramble shRNA or shRNA specific against Beclin1 (shBeclin1), and Western blot analysis was performed using the indicated antibodies. b HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct were treated with vehicle, 3-MA or WM respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing EGFP-LC3B were infected with lentivirus containing empty or BAG3 construct. Then cells were treated with vehicle, 3-MA or WM, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. * P < 0.05. N.S. not significant. Error bars indicate means ± SD

Journal: Cell Death & Disease

Article Title: BAG3 promotes autophagy and glutaminolysis via stabilizing glutaminase

doi: 10.1038/s41419-019-1504-6

Figure Lengend Snippet: a Control or BAG3-overexpressing HepG2 and MCF7 cells were transfected with scramble shRNA or shRNA specific against Beclin1 (shBeclin1), and Western blot analysis was performed using the indicated antibodies. b HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct were treated with vehicle, 3-MA or WM respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing EGFP-LC3B were infected with lentivirus containing empty or BAG3 construct. Then cells were treated with vehicle, 3-MA or WM, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of ( c ). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. * P < 0.05. N.S. not significant. Error bars indicate means ± SD

Article Snippet: Antibodies were used against the following: GLS (Sigma-Aldrich, WH0002744M1), GLS (Abcam, ab156876), GAPDH (Merck-Millipore, AB2302), LC3B (Origene, am20213pu), P62 (BD Biosciences, 610833), Beclin1 (Cell Signaling Technology, 3495), ATG3 (Cell Signaling Technology, 3415), ATG5 (Cell Signaling Technology, 12994), ATG7 (Cell Signaling Technology, 8558), ATG12 (Cell Signaling Technology, 4180), SIRT5 (Cell Signaling Technology, 8779), c-Myc (Invitrogen, R950-25), DYKDDDDK Tag (Cell Signaling Technology, 14793), HA-Tag (Cell Signaling Technology, 3724), pan-succinylation (PTM Biolabs, Hangzhou).

Techniques: Transfection, shRNA, Western Blot, Transduction, Construct, Expressing, Stable Transfection, Infection, Microscopy